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Appl Environ Microbiol
1989 Oct 01;5510:2474-9. doi: 10.1128/aem.55.10.2474-2479.1989.
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19F nuclear magnetic resonance analysis of 5-fluorouracil metabolism in wild-type and 5-fluorouracil-resistant Nectria haematococca.
Parisot D
,
Malet-Martino MC
,
Crasnier P
,
Martino R
.
Abstract
A mutant (furA3) was isolated from the S1 wild-type strain of Nectria haematococca on the basis of its resistance to 5-fluorouracil (5FU). This mutant has greatly reduced activity of uracil phosphoribosyltransferase, a pyrimidine salvage enzyme catalyzing the synthesis of UMP from uracil. The metabolism of 5FU was examined in both strains by using 19F nuclear magnetic resonance spectroscopy. In the S1 strain, 5FU appears to be metabolized by two pathways operating simultaneously: (i) conversion to fluoronucleotides and (ii) degradation into alpha-fluoro-beta-alanine. The furA3 mutant shows metabolic changes consistent with a uracil phosphoribosyltransferase lesion, since it takes up 5FU and forms a small amount of alpha-fluoro-beta-alanine but does not synthesize fluoronucleotides. Since pigment synthesis is strongly enhanced by 5FU in the S1 wild-type strain but not in the furA3 mutant, these results support the hypothesis that 5FU stimulation of secondary metabolism in N. haematococca is not mediated by the drug itself but involves a phosphorylated anabolite.
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976, Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Di Vito,
A 19F nuclear magnetic resonance study of uptake and metabolism of 5-fluorocytosine in susceptible and resistant strains of Candida albicans.
1986,
Pubmed
Dreyer,
Use of periodate and methylamine for the quantitation of intracellular 5-fluoro-2'-deoxyuridine-5'-monophosphate by high-performance liquid chromatography.
1981,
Pubmed
Galun,
Morphogenesis in Trichoderma: suppression of photoinduction by 5-fluorouracil.
1966,
Pubmed
Garrett,
A rapid and sensitive high pressure liquid chromatography assay for deoxyribonucleoside triphosphates in cell extracts.
1979,
Pubmed
Gochin,
In vivo 19F-NMR of 5-fluorouracil incorporation into RNA and metabolites in Escherichia coli cells.
1984,
Pubmed
Heidelberger,
Fluorinated pyrimidines and their nucleosides.
1983,
Pubmed
Horowitz,
Formation of active beta-galactosidase by Escherichia coli treated with 5-fluorouracil.
1967,
Pubmed
Jones,
6-azauracil-resistant variants of cultured plant cells lack uracil phosphoribosyltransferase activity.
1984,
Pubmed
Jund,
Genetic and physiological aspects of resistance to 5-fluoropyrimidines in Saccharomyces cerevisiae.
1970,
Pubmed
Keniry,
Noninvasive spectroscopic analysis of fluoropyrimidine metabolism in cultured tumor cells.
1986,
Pubmed
Lindenmayer,
Selective effects of purine and pyrimidine analogues and of respiratory inhibitors on perithecial development and branching in sordaria.
1967,
Pubmed
Malet-Martino,
Noninvasive fluorine-19 NMR study of fluoropyrimidine metabolism in cell cultures of human pancreatic and colon adenocarcinoma.
1986,
Pubmed
O'Byrne-Ring,
Biochemical and genetic basis of the response to 5-fluorouracil in Drosophila melanogaster.
1980,
Pubmed
Palmer,
Pyrimidine biosynthesis in Aspergillus nidulans. Isolation and characterisation of mutants resistant to fluoropyrimidines.
1975,
Pubmed
Parente,
Effect of DNA inhibitors upon DNA synthesis and development of Drosophila embryos.
1980,
Pubmed
Valeriote,
5-Fluorouracil (FUra).
1984,
Pubmed
Vialaneix,
Noninvasive and quantitative 19F nuclear magnetic resonance study of flucytosine metabolism in Candida strains.
1986,
Pubmed
Wain,
Rates of synthesis of ribosomal protein and total ribonucleic acid through the cell cycle of the fission yeast Schizosaccharomyces pombe.
1973,
Pubmed
Waldorf,
Mechanisms of action of 5-fluorocytosine.
1983,
Pubmed
