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Mar Drugs
2016 Sep 30;1410:. doi: 10.3390/md14100176.
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Angiotensin-I Converting Enzyme (ACE) Inhibitory and Anti-Hypertensive Effect of Protein Hydrolysate from Actinopyga lecanora (Sea Cucumber) in Rats.
Sadegh Vishkaei M
,
Ebrahimpour A
,
Abdul-Hamid A
,
Ismail A
,
Saari N
.
Abstract
Food protein hydrolysates are known to exhibit angiotensin converting enzyme (ACE) inhibitory properties and can be used as a novel functional food for prevention of hypertension. This study evaluated the ACE inhibitory potentials of Actinopyga lecanora proteolysate (ALP) in vivo. The pre-fed rats with ALP at various doses (200, 400, 800 mg/kg body weight) exhibited a significant (p ≤ 0.05) suppression effect after inducing hypertension. To determine the optimum effective dose that will produce maximal reduction in blood pressure, ALP at three doses was fed to the rats after inducing hypertension. The results showed that the 800 mg/kg body weight dose significantly reduced blood pressure without noticeable negative physiological effect. In addition, there were no observable changes in the rats'' heart rate after oral administration of the ALP. It was concluded that Actinopyga lecanora proteolysate could potentially be used for the development of functional foods and nutraceuticals for prevention and treatment of hypertension.
Figure 1. The effect of proteolysate (200, 400, 800 mg/kg body weight) on normal blood pressure. Positive group was given captopril (50 mg/kg body weight). Water control group was given distilled water. ΔP (mm Hg) shows blood pressure changes, which can be increase or decrease; (−) shows decrease and (+) shows increase in blood pressure. A–C significant differences at the confidence level of p ≤ 0.05 (mean ± SD, N = 5), (a) SBP; (b) DBP.
Figure 2. ΔP (mm Hg) showing blood pressure changes before and after inducing HBP via injections of angiotensin I (0.3 μg/kg body weight) and saline. Treated rats were given different doses of Actinopyga lecanora proteolysate (ALP) (200, 400, 800 mg/kg body weight) and positive group was given captopril (50 mg/kg body weight), (mean ± SD, N = 5), (a) SBP; (b) DBP.
Figure 3. The effect of ALP at doses of 200, 400, 800 mg/kg body weight in pre-fed rats, after inducing hypertension by angiotensin I (0.3 μg/kg body weight) injection. The positive group was given captopril (50 mg/kg body weight). ΔP (mm Hg) shows blood pressure changes, which can be increase or decrease; (−) shows decrease and (+) shows increase in blood pressure. A–D significant differences at the confidence level of p ≤ 0.05 (mean ± SD, N = 5), (a) SBP; (b) DBP.
Figure 4. ΔP (mm Hg) showing blood pressure changes before and after inducing HBP via injections of angiotensin I (0.3 μg/kg body weight) and saline. Treated rats were given different doses of ALP (200, 400, 800 mg/kg body weight) and positive group was given captopril (50 mg/kg body weight), (mean ± SD, N = 5), (a) SBP; (b) DBP.
Figure 5. The effect of ALP at doses of 200, 400, 800 mg/kg body weight after inducing hypertension by angiotensin I (0.3 μg/kg body weight) injection. The positive group was given captopril (50 mg/kg body weight). ΔP (mm Hg) shows blood pressure changes, which can be increase or decrease; (−) shows decrease and (+) shows increase in blood pressure. A–D significant differences at the confidence level of p ≤ 0.05 (mean ± SD, N = 5), (a) SBP, (b) DBP.
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