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Springerplus
2014 Nov 14;3:673. doi: 10.1186/2193-1801-3-673.
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Bioactive compounds from Holothuria atra of Indian ocean.
Dhinakaran DI
,
Lipton AP
.
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The sea cucumber (Holothuria atra) extracts have been evaluated for the presence of bioactive compounds and various biological activities. The methanol extracts showed anti proliferative activities against the Hela and MCF-7 cell lines. Similarly the inhibitory effects of Herpes simplex virus 1 and 2 cells were detected using the plaque reduction assay. The extracts of H. atra were purified using the silica gel column chromatography. The active fractions collected were observed for antimicrobial activity. The GC-MS analysis showed the availability of 59 compounds. The active bioactive compounds found in the H. atra were analyzed and their structure was identified using the (1)HNMR and (13)C NMR experiments.
Figure 1.
Cytotoxic effects of
H.atra
extracts on A) Hela and B) MCF-7 cell lines.
Figure 2.
In vitro
antiviral activity of
Holothuria atra
extracts against A) HSV-1and B) HSV-2 using plaque reduction assay.
Figure 3.
Anti bacterial activity of the column purified fractions of sea cucumber,
Holothuria atra
against A)
Serratia liquefaciens
B)
Escherichia coli
C)
Klebsiella pneumoniae
D)
Staphylococcus aureus.
Figure 4.
GC-MS Analysis of the methanolic extracts of the sea cucumber
Holothuria atra.
Figure 5.
NMR analysis of active fractions obtained from Column chromatography (A)
1
H NMR Spectrum of
Holothuria atra
(B)
1
H NMR Spectrum of
Holothuria atra
(C)
13
C NMR Spectrum of
Holothuria atra.
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