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Cellular and biochemical responses to environmental and experimentally induced stress in sea urchin coelomocytes.
Matranga V
,
Toia G
,
Bonaventura R
,
Müller WE
.
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Coelomocytes are considered to be immune effectors of sea urchins. Subpopulations of coelomocytes can be purified from a total cell suspension. The proportion of each cell type can vary not only among species, but also between individuals of the same species, according to their size and physiological conditions. We tested the hypothesis that coelomocytes play a role in defense mechanisms activated by adverse external conditions. Total coelomocytes from control and stressed (temperature, pollution, and injuries) sea urchins were analyzed for their expression of the 70 kDa heat shock protein (hsp70), a well recognized stress marker. Further analysis was performed by separation of coelomocytes into subpopulations by step gradients. We demonstrated that sea urchin coelomocytes respond to temperature shock and to polluted seawater by the upregulation of hsp70. Among coelomocytes certain cells, known as red spherula cells, showed a great increase in number in animals collected from polluted seawaters or subjected to "accidental" injury. The present study confirms the immunological function of sea urchin coelomocytes, as indicated by the upregulation of the hsp70 molecular marker, and suggests that sea urchin coelomocytes can be utilized as sensitive bio-indicators of environmental stress.
Al-Sharif,
Sea urchin coelomocytes specifically express a homologue of the complement component C3.
1998, Pubmed,
Echinobase
Al-Sharif,
Sea urchin coelomocytes specifically express a homologue of the complement component C3.
1998,
Pubmed
,
Echinobase
Beck,
Invertebrate cytokines: the phylogenetic emergence of interleukin-1.
1989,
Pubmed
Beck,
Characterization of an IL-6-like molecule from an echinoderm (Asterias forbesi).
1996,
Pubmed
,
Echinobase
Beck,
Isolation and characterization of a primitive interleukin-1-like protein from an invertebrate, Asterias forbesi.
1986,
Pubmed
,
Echinobase
Cervello,
Detection of vitellogenin in a subpopulation of sea urchin coelomocytes.
1994,
Pubmed
,
Echinobase
Garcia-Pichel,
Evidence for an ultraviolet sunscreen role of the extracellular pigment scytonemin in the terrestrial cyanobacterium Chlorogloeopsis sp.
1992,
Pubmed
Giudice,
Heat Shock Proteins in Sea Urchin Embryos: (heat shock proteins/sea urchin embryos).
1989,
Pubmed
,
Echinobase
Goldschmidt-Clermont,
Mechanism of the interaction of human platelet profilin with actin.
1991,
Pubmed
Johnson,
The coelomic elements of sea urchins (Strongylocentrotus). 3. In vitro reaction to bacteria.
1969,
Pubmed
,
Echinobase
Kurelec,
The multixenobiotic resistance mechanism in aquatic organisms.
1992,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Matranga,
Molecular aspects of immune reactions in Echinodermata.
1996,
Pubmed
,
Echinobase
Morale,
Biological effects of a neurotoxic pesticide at low concentrations on sea urchin early development. A terathogenic assay.
1998,
Pubmed
,
Echinobase
Pagliara,
Isolation of cytolytic granules from sea urchin amoebocytes.
1993,
Pubmed
,
Echinobase
Sconzo,
Effect of doxorubicin and phenytoin on sea urchin development.
1995,
Pubmed
,
Echinobase
Sconzo,
Characterization of a new member of the sea urchin Paracentrotus lividus hsp70 gene family and its expression.
1992,
Pubmed
,
Echinobase
Smith,
Sea urchin genes expressed in activated coelomocytes are identified by expressed sequence tags. Complement homologues and other putative immune response genes suggest immune system homology within the deuterostomes.
1996,
Pubmed
,
Echinobase
Smith,
Coelomocytes express SpBf, a homologue of factor B, the second component in the sea urchin complement system.
1998,
Pubmed
,
Echinobase
Smith,
Sensitization and histamine release by cells of the sand dollar, Mellita quinquiesperforata.
1985,
Pubmed
,
Echinobase
Smith,
The echinoid immune system and the phylogenetic occurrence of immune mechanisms in deuterostomes.
1992,
Pubmed
,
Echinobase
Smith,
SpCoel1: a sea urchin profilin gene expressed specifically in coelomocytes in response to injury.
1992,
Pubmed
,
Echinobase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Zhu,
Endogenous substance P mediates cold water stress-induced increase in interleukin-6 secretion from peritoneal macrophages.
1996,
Pubmed
