Biol Bull 1991 Aug 01;1811:95-103. doi: 10.2307/1542492.

Immunological Survey of Planktonic Embryos and Larvae of the Starfish Asterina pectinifera, Obtained from the Sea, Using a Monoclonal Antibody Directed against Egg Polypeptides.

Abstract
A monoclonal antibody, K1, specifically recognizes polypeptides with apparent molecular masses of 56 and 58 kDa, in the egg of the starfish Asterina pectinifera. The K1 antibody reacted with extracts prepared from ovaries, oocytes, morulae, blastulae, gastrulae, and bipinnariae. Brachiolariae, testes, pyloric ceca, body walls, and tubefeet did not contain K1-reactive antigenic molecules. Extracts of eggs of the starfish Asterias amurensis and several sea urchin species did not react with the K1 antibody. Among the members of the genus Asterina, extracts of brachiolariae of A. batheri and A. minor were not reactive, whereas blastulae and brachiolariae of A. pseudoexigua pacifica did contain an antigenic component. Unlike the antigenic peptides in A. pectinifera eggs, the apparent molecular mass of the antigen molecule in embryos and larvae of A. pseudoexigua pacifica was 41 kDa, which represents a remarkable phylogenetic variation of antigen molecules. Using the K1 antibody, we have developed an assay system that detects embryos and larvae of A. pectinifera in complex mixtures of biological specimens obtained from the sea.

PubMed ID: 29303655
Article link: Biol Bull


Genes referenced: LOC100887844