Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Dev Biol
2007 Jun 15;3062:860-9. doi: 10.1016/j.ydbio.2007.03.033.
Show Gene links
Show Anatomy links
Cis-regulatory control of the nodal gene, initiator of the sea urchin oral ectoderm gene network.
Nam J
,
Su YH
,
Lee PY
,
Robertson AJ
,
Coffman JA
,
Davidson EH
.
???displayArticle.abstract???
Expression of the nodal gene initiates the gene regulatory network which establishes the transcriptional specification of the oral ectoderm in the sea urchin embryo. This gene encodes a TGFbeta ligand, and in Strongylocentrotus purpuratus its transcription is activated in the presumptive oral ectoderm at about the 30-cell stage. Thereafter Nodal signaling occurs among all cells of the oral ectoderm territory, and nodal expression is required for expression of oral ectoderm regulatory genes. The cis-regulatory system of the nodal gene transduces anisotropically distributed cytoplasmic cues that distinguish the future oral and aboral domains of the early embryo. Here we establish the genomic basis for the initiation and maintenance of nodal gene expression in the oral ectoderm. Functional cis-regulatory control modules of the nodal gene were identified by interspecific sequence conservation. A 5'' cis-regulatory module functions both to initiate expression of the nodal gene and to maintain its expression by means of feedback input from the Nodal signal transduction system. These functions are mediated respectively by target sites for bZIP transcription factors, and by SMAD target sites. At least one SMAD site is also needed for the initiation of expression. An intron module also contains SMAD sites which respond to Nodal feedback, and in addition acts to repress vegetal expression. These observations explain the main features of nodal expression in the oral ectoderm: since the activity of bZIP factors is redox sensitive, and the initial polarization of oral vs. aboral fate is manifested in a redox differential, the bZIP sites account for the activation of nodal on the oral side; and since the immediate early signal transduction response factors for Nodal are SMAD factors, the SMAD sites account for the feedback maintenance of nodal gene expression.
Abate,
Redox regulation of fos and jun DNA-binding activity in vitro.
1990, Pubmed
Abate,
Redox regulation of fos and jun DNA-binding activity in vitro.
1990,
Pubmed
Amoutzias,
Reduction/oxidation-phosphorylation control of DNA binding in the bZIP dimerization network.
2006,
Pubmed
Arnone,
Green Fluorescent Protein in the sea urchin: new experimental approaches to transcriptional regulatory analysis in embryos and larvae.
1997,
Pubmed
,
Echinobase
Bradham,
p38 MAPK is essential for secondary axis specification and patterning in sea urchin embryos.
2006,
Pubmed
,
Echinobase
Brown,
Paircomp, FamilyRelationsII and Cartwheel: tools for interspecific sequence comparison.
2005,
Pubmed
Cameron,
Segregation of oral from aboral ectoderm precursors is completed at fifth cleavage in the embryogenesis of Strongylocentrotus purpuratus.
1990,
Pubmed
,
Echinobase
Cartharius,
MatInspector and beyond: promoter analysis based on transcription factor binding sites.
2005,
Pubmed
Coffman,
Oral-aboral axis specification in the sea urchin embryo. I. Axis entrainment by respiratory asymmetry.
2001,
Pubmed
,
Echinobase
Coffman,
Oral-aboral axis specification in the sea urchin embryo II. Mitochondrial distribution and redox state contribute to establishing polarity in Strongylocentrotus purpuratus.
2004,
Pubmed
,
Echinobase
Duboc,
Nodal and BMP2/4 signaling organizes the oral-aboral axis of the sea urchin embryo.
2004,
Pubmed
,
Echinobase
Flowers,
Nodal/activin signaling establishes oral-aboral polarity in the early sea urchin embryo.
2004,
Pubmed
,
Echinobase
Gurdon,
A community effect in animal development.
1989,
Pubmed
Howard-Ashby,
Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Inoue,
The C. elegans p38 MAPK pathway regulates nuclear localization of the transcription factor SKN-1 in oxidative stress response.
2005,
Pubmed
Istrail,
Logic functions of the genomic cis-regulatory code.
2005,
Pubmed
,
Echinobase
Koide,
Xenopus as a model system to study transcriptional regulatory networks.
2005,
Pubmed
,
Echinobase
Lee,
A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.
2001,
Pubmed
Livant,
Differential stability of expression of similarly specified endogenous and exogenous genes in the sea urchin embryo.
1991,
Pubmed
,
Echinobase
Materna,
The C2H2 zinc finger genes of Strongylocentrotus purpuratus and their expression in embryonic development.
2006,
Pubmed
,
Echinobase
Minokawa,
cis-Regulatory inputs of the wnt8 gene in the sea urchin endomesoderm network.
2005,
Pubmed
,
Echinobase
Revilla-i-Domingo,
R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres.
2004,
Pubmed
,
Echinobase
Sakuma,
Inhibition of Nodal signalling by Lefty mediated through interaction with common receptors and efficient diffusion.
2002,
Pubmed
Schier,
Nodal signaling in vertebrate development.
2003,
Pubmed
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Wikramanayake,
beta-Catenin is essential for patterning the maternally specified animal-vegetal axis in the sea urchin embryo.
1998,
Pubmed
,
Echinobase
Wikramanayake,
Autonomous and non-autonomous differentiation of ectoderm in different sea urchin species.
1995,
Pubmed
,
Echinobase
Wikramanayake,
Multiple signaling events specify ectoderm and pattern the oral-aboral axis in the sea urchin embryo.
1997,
Pubmed
,
Echinobase
Wong,
Real-time PCR for mRNA quantitation.
2005,
Pubmed
Yaguchi,
Sp-Smad2/3 mediates patterning of neurogenic ectoderm by nodal in the sea urchin embryo.
2007,
Pubmed
,
Echinobase
Yon,
Precise gene fusion by PCR.
1989,
Pubmed