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J Zhejiang Univ Sci B
2011 Jul 01;127:534-44. doi: 10.1631/jzus.B1000217.
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Ds-echinoside A, a new triterpene glycoside derived from sea cucumber, exhibits antimetastatic activity via the inhibition of NF-κB-dependent MMP-9 and VEGF expressions.
Zhao Q
,
Liu ZD
,
Xue Y
,
Wang JF
,
Li H
,
Tang QJ
,
Wang YM
,
Dong P
,
Xue CH
.
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Ds-echinoside A (DSEA), a non-sulfated triterpene glycoside, was isolated from the sea cucumber Pearsonothuria graeffei. In vitro and in vivo investigations were conducted on the effects of DSEA on tumor cell adhesion, migration, invasion, and angiogenesis. In this study, we found that DSEA inhibited the proliferation of human hepatocellular liver carcinoma cells Hep G2, with a half-maximal inhibitory concentration (IC₅₀) of 2.65 μmol/L, and suppressed Hep G2 cell adhesion, migration, and invasion in a dose-dependent manner. DSEA also reduced tube formation of human endothelial cells ECV-304 on matrigel in vitro and attenuated neovascularization in the chick embryo chorioallantoic membrane (CAM) assay in vivo. Immunocytochemical analysis revealed that DSEA significantly decreased the expression of matrix metalloproteinase-9 (MMP-9), which plays an important role in the degradation of basement membrane in tumor metastasis and angiogenesis. DSEA also increased the protein expression level of tissue inhibitor of metalloproteinase-1 (TIMP-1), an important regulator of MMP-9 activation. From the results of Western blotting, the expressions of nuclear factor-kappa B (NF-κB) and vascular endothelial growth factor (VEGF) were found to be remarkably reduced by DSEA. These findings suggest that DSEA exhibits a significant anti-metastatic activity through the specific inhibition of NF-κB-dependent MMP-9 and VEGF expressions.
Alaniz,
Modulation of matrix metalloproteinase-9 activity by hyaluronan is dependent on NF-kappaB activity in lymphoma cell lines with dissimilar invasive behavior.
2004, Pubmed
Alaniz,
Modulation of matrix metalloproteinase-9 activity by hyaluronan is dependent on NF-kappaB activity in lymphoma cell lines with dissimilar invasive behavior.
2004,
Pubmed
Alferez,
Dual inhibition of VEGFR and EGFR signaling reduces the incidence and size of intestinal adenomas in Apc(Min/+) mice.
2008,
Pubmed
Chambers,
Dissemination and growth of cancer cells in metastatic sites.
2002,
Pubmed
Chan,
Intestinal trefoil factor promotes invasion in non-tumorigenic Rat-2 fibroblast cell.
2005,
Pubmed
Chang,
The many faces of metalloproteases: cell growth, invasion, angiogenesis and metastasis.
2001,
Pubmed
Chludil,
Cytotoxic and antifungal triterpene glycosides from the Patagonian sea cucumber Hemoiedema spectabilis.
2002,
Pubmed
,
Echinobase
Choi,
Suppression of PMA-induced tumor cell invasion and metastasis by aqueous extract isolated from Prunella vulgaris via the inhibition of NF-kappaB-dependent MMP-9 expression.
2010,
Pubmed
Dai,
TWEAK promotes ovarian cancer cell metastasis via NF-kappaB pathway activation and VEGF expression.
2009,
Pubmed
Dong,
Determination of triterpene glycosides in sea cucumber (Stichopus japonicus) and its related products by high-performance liquid chromatography.
2008,
Pubmed
,
Echinobase
Farina,
Transcriptional up-regulation of matrix metalloproteinase-9 expression during spontaneous epithelial to neuroblast phenotype conversion by SK-N-SH neuroblastoma cells, involved in enhanced invasivity, depends upon GT-box and nuclear factor kappaB elements.
1999,
Pubmed
Folkman,
Angiogenesis.
2006,
Pubmed
Forsythe,
Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1.
1996,
Pubmed
Hornebeck,
Beneficial and detrimental influences of tissue inhibitor of metalloproteinase-1 (TIMP-1) in tumor progression.
2005,
Pubmed
Imanaka,
Chemoprevention of tumor metastasis by liposomal beta-sitosterol intake.
2008,
Pubmed
Jiang,
Complex roles of tissue inhibitors of metalloproteinases in cancer.
2002,
Pubmed
Jin,
Differential effects of triterpene glycosides, frondoside A and cucumarioside A2-2 isolated from sea cucumbers on caspase activation and apoptosis of human leukemia cells.
2009,
Pubmed
,
Echinobase
Kaomongkolgit,
Iron increases MMP-9 expression through activation of AP-1 via ERK/Akt pathway in human head and neck squamous carcinoma cells.
2008,
Pubmed
Kiriakidis,
VEGF expression in human macrophages is NF-kappaB-dependent: studies using adenoviruses expressing the endogenous NF-kappaB inhibitor IkappaBalpha and a kinase-defective form of the IkappaB kinase 2.
2003,
Pubmed
Kitagawa,
Marine natural products. XIV. Structures of echinosides A and B, antifungal lanostane-oligosides from the sea cucumber Actinopyga echinites (Jaeger).
1985,
Pubmed
,
Echinobase
Kleiner,
Matrix metalloproteinases and metastasis.
1999,
Pubmed
Ko,
Platelet-activating factor-induced NF-kappaB activation enhances VEGF expression through a decrease in p53 activity.
2006,
Pubmed
La Porta,
PKC-dependent modulation of IkB alpha-NFkB pathway in low metastatic B16F1 murine melanoma cells and in highly metastatic BL6 cells.
1998,
Pubmed
Lee,
Saponins derived from the roots of Platycodon grandiflorum inhibit HT-1080 cell invasion and MMPs activities: regulation of NF-kappaB activation via ROS signal pathway.
2008,
Pubmed
Leung,
Vascular endothelial growth factor is a secreted angiogenic mitogen.
1989,
Pubmed
Liaw,
Osteopontin promotes vascular cell adhesion and spreading and is chemotactic for smooth muscle cells in vitro.
1994,
Pubmed
Liotta,
Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation.
1991,
Pubmed
Liu,
Arguside A: a new cytotoxic triterpene glycoside from the sea cucumber Bohadschia argus Jaeger.
2007,
Pubmed
,
Echinobase
Machesky,
Lamellipodia and filopodia in metastasis and invasion.
2008,
Pubmed
McCawley,
Matrix metalloproteinases: multifunctional contributors to tumor progression.
2000,
Pubmed
Nabeshima,
Matrix metalloproteinases in tumor invasion: role for cell migration.
2002,
Pubmed
Nagao,
Enhanced invasion of Tax-expressing fibroblasts into the basement membrane is repressed by phosphorylated ascorbate with simultaneous decreases in intracellular oxidative stress and NF-kappa B activation.
2000,
Pubmed
Pahl,
Activators and target genes of Rel/NF-kappaB transcription factors.
1999,
Pubmed
Pasco,
Control of melanoma cell invasion by type IV collagen.
2005,
Pubmed
Rao,
Molecular mechanisms of glioma invasiveness: the role of proteases.
2003,
Pubmed
Sancéau,
Matrix metalloproteinase-9 silencing by RNA interference triggers the migratory-adhesive switch in Ewing's sarcoma cells.
2003,
Pubmed
Shibata,
Inhibition of NF-kappaB activity decreases the VEGF mRNA expression in MDA-MB-231 breast cancer cells.
2002,
Pubmed
Skehan,
New colorimetric cytotoxicity assay for anticancer-drug screening.
1990,
Pubmed
Steeg,
Tumor metastasis: mechanistic insights and clinical challenges.
2006,
Pubmed
Tan,
A small peptide derived from Flt-1 (VEGFR-1) functions as an angiogenic inhibitor.
2001,
Pubmed
Tian,
PE, a new sulfated saponin from sea cucumber, exhibits anti-angiogenic and anti-tumor activities in vitro and in vivo.
2005,
Pubmed
,
Echinobase
Tong,
Philinopside A, a novel marine-derived compound possessing dual anti-angiogenic and anti-tumor effects.
2005,
Pubmed
,
Echinobase
Wang,
Inhibiting colorectal carcinoma growth and metastasis by blocking the expression of VEGF using RNA interference.
2008,
Pubmed
Watanabe,
Matrix metalloproteinase-9 (92 kDa gelatinase/type IV collagenase) from U937 monoblastoid cells: correlation with cellular invasion.
1993,
Pubmed
Westermarck,
Regulation of matrix metalloproteinase expression in tumor invasion.
1999,
Pubmed
Yu,
NF-kappaB as a molecular target in adjuvant therapy of gastrointestinal carcinomas.
2005,
Pubmed
Zhang,
Imbalance between expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in invasiveness and metastasis of human gastric carcinoma.
2003,
Pubmed
Zhang,
Cytotoxic triterpene glycosides from the sea cucumber Pseudocolochirus violaceus.
2007,
Pubmed
,
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