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Development
2014 Dec 01;14123:4598-609. doi: 10.1242/dev.113563.
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Two-pore channels function in calcium regulation in sea star oocytes and embryos.
Ramos I
,
Reich A
,
Wessel GM
.
???displayArticle.abstract??? Egg activation at fertilization is an excellent process for studying calcium regulation. Nicotinic acid adenine dinucleotide-phosphate (NAADP), a potent calcium messenger, is able to trigger calcium release, likely through two-pore channels (TPCs). Concomitantly, a family of ectocellular enzymes, the ADP-ribosyl cyclases (ARCs), has emerged as being able to change their enzymatic mode from one of nucleotide cyclization in formation of cADPR to a base-exchange reaction in the generation of NAADP. Using sea star oocytes we gain insights into the functions of endogenously expressed TPCs and ARCs in the context of the global calcium signals at fertilization. Three TPCs and one ARC were found in the sea star (Patiria miniata) that were localized in the cortex of the oocytes and eggs. PmTPCs were localized in specialized secretory organelles called cortical granules, and PmARCs accumulated in a different, unknown, set of vesicles, closely apposed to the cortical granules in the egg cortex. Using morpholino knockdown of PmTPCs and PmARC in the oocytes, we found that both calcium regulators are essential for early embryo development, and that knockdown of PmTPCs leads to aberrant construction of the fertilization envelope at fertilization and changes in cortical granule pH. The calcium signals at fertilization are not significantly altered when individual PmTPCs are silenced, but the timing and shape of the cortical flash and calcium wave are slightly changed when the expression of all three PmTPCs is perturbed concomitantly, suggesting a cooperative activity among TPC isoforms in eliciting calcium signals that may influence localized physiological activities.
Alliegro,
Immunocytochemical localization of the 35-kDa sea urchin egg trypsin-like protease and its effects upon the egg surface.
1988, Pubmed,
Echinobase
Alliegro,
Immunocytochemical localization of the 35-kDa sea urchin egg trypsin-like protease and its effects upon the egg surface.
1988,
Pubmed
,
Echinobase
Arndt,
NAADP and the two-pore channel protein 1 participate in the acrosome reaction in mammalian spermatozoa.
2014,
Pubmed
Arredouani,
An emerging role for NAADP-mediated Ca2+ signaling in the pancreatic β-cell.
2010,
Pubmed
Berg,
Nicotinic acid adenine dinucleotide phosphate (NAADP(+)) is an essential regulator of T-lymphocyte Ca(2+)-signaling.
2000,
Pubmed
Brailoiu,
An NAADP-gated two-pore channel targeted to the plasma membrane uncouples triggering from amplifying Ca2+ signals.
2010,
Pubmed
Brailoiu,
Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling.
2009,
Pubmed
Brailoiu,
An ancestral deuterostome family of two-pore channels mediates nicotinic acid adenine dinucleotide phosphate-dependent calcium release from acidic organelles.
2010,
Pubmed
,
Echinobase
Calcraft,
NAADP mobilizes calcium from acidic organelles through two-pore channels.
2009,
Pubmed
Cang,
mTOR regulates lysosomal ATP-sensitive two-pore Na(+) channels to adapt to metabolic state.
2013,
Pubmed
Carafoli,
Generation, control, and processing of cellular calcium signals.
2001,
Pubmed
Churamani,
Molecular characterization of a novel intracellular ADP-ribosyl cyclase.
2007,
Pubmed
,
Echinobase
Churamani,
Molecular characterization of a novel cell surface ADP-ribosyl cyclase from the sea urchin.
2008,
Pubmed
,
Echinobase
Churchill,
NAADP mobilizes Ca(2+) from reserve granules, lysosome-related organelles, in sea urchin eggs.
2002,
Pubmed
,
Echinobase
Cuéllar-Mata,
The GTP-binding protein RhoA localizes to the cortical granules of Strongylocentrotus purpuratas sea urchin egg and is secreted during fertilization.
2000,
Pubmed
,
Echinobase
Davis,
NAADP activates two-pore channels on T cell cytolytic granules to stimulate exocytosis and killing.
2012,
Pubmed
Davis,
Cytolytic granules supply Ca(2+) for their own exocytosis via NAADP and resident two-pore channels.
2013,
Pubmed
Davis,
Ca(2+) signaling occurs via second messenger release from intraorganelle synthesis sites.
2008,
Pubmed
,
Echinobase
Dunn,
Agalma: an automated phylogenomics workflow.
2013,
Pubmed
Guida,
Equilibrative and concentrative nucleoside transporters mediate influx of extracellular cyclic ADP-ribose into 3T3 murine fibroblasts.
2002,
Pubmed
Guse,
Linking NAADP to ion channel activity: a unifying hypothesis.
2012,
Pubmed
Hara-Yokoyama,
Tetrameric interaction of the ectoenzyme CD38 on the cell surface enables its catalytic and raft-association activities.
2012,
Pubmed
Henson,
Differentiation of a calsequestrin-containing endoplasmic reticulum during sea urchin oogenesis.
1990,
Pubmed
,
Echinobase
Holland,
Electron microscopic study of the cortical reaction in eggs of the starfish (Patria miniata).
1980,
Pubmed
,
Echinobase
Hooper,
Membrane topology of NAADP-sensitive two-pore channels and their regulation by N-linked glycosylation.
2011,
Pubmed
Hylander,
An ultrastructural immunocytochemical localization of hyalin in the sea urchin egg.
1982,
Pubmed
,
Echinobase
Kishimoto,
A primer on meiotic resumption in starfish oocytes: the proposed signaling pathway triggered by maturation-inducing hormone.
2011,
Pubmed
,
Echinobase
Lee,
Physiological functions of cyclic ADP-ribose and NAADP as calcium messengers.
2001,
Pubmed
Lee,
Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate (NAADP) as messengers for calcium mobilization.
2012,
Pubmed
Lim,
NAADP+ initiates the Ca2+ response during fertilization of starfish oocytes.
2001,
Pubmed
,
Echinobase
Lu,
NAADP/TPC2/Ca(2+) Signaling Inhibits Autophagy.
2013,
Pubmed
Lu,
Two pore channel 2 (TPC2) inhibits autophagosomal-lysosomal fusion by alkalinizing lysosomal pH.
2013,
Pubmed
Macgregor,
NAADP controls cross-talk between distinct Ca2+ stores in the heart.
2007,
Pubmed
Moccia,
Pharmacological characterization of NAADP-induced Ca2+ signals in starfish oocytes.
2006,
Pubmed
,
Echinobase
Moccia,
NAADP and InsP3 play distinct roles at fertilization in starfish oocytes.
2006,
Pubmed
,
Echinobase
Morgan,
Molecular mechanisms of endolysosomal Ca2+ signalling in health and disease.
2011,
Pubmed
Morgan,
Sea urchin eggs in the acid reign.
2011,
Pubmed
,
Echinobase
Morgan,
Two-pore channels (TPCs): current controversies.
2014,
Pubmed
Morgan,
NAADP induces pH changes in the lumen of acidic Ca2+ stores.
2007,
Pubmed
,
Echinobase
Morgan,
Bidirectional Ca²⁺ signaling occurs between the endoplasmic reticulum and acidic organelles.
2013,
Pubmed
,
Echinobase
Morgan,
Fertilization and nicotinic acid adenine dinucleotide phosphate induce pH changes in acidic Ca(2+) stores in sea urchin eggs.
2007,
Pubmed
,
Echinobase
Oulhen,
Dysferlin is essential for endocytosis in the sea star oocyte.
2014,
Pubmed
,
Echinobase
Oulhen,
Diversity in the fertilization envelopes of echinoderms.
2013,
Pubmed
,
Echinobase
Parrington,
Ca(2+) signals, NAADP and two-pore channels: role in cellular differentiation.
2014,
Pubmed
Patel,
Acidic calcium stores open for business: expanding the potential for intracellular Ca2+ signaling.
2010,
Pubmed
Patel,
Acidic Ca(2+) stores come to the fore.
2011,
Pubmed
Patel,
The endo-lysosomal system as an NAADP-sensitive acidic Ca(2+) store: role for the two-pore channels.
2011,
Pubmed
Peiter,
The vacuolar Ca2+-activated channel TPC1 regulates germination and stomatal movement.
2005,
Pubmed
Pitt,
TPC2 is a novel NAADP-sensitive Ca2+ release channel, operating as a dual sensor of luminal pH and Ca2+.
2010,
Pubmed
Ploegh,
A lipid-based model for the creation of an escape hatch from the endoplasmic reticulum.
2007,
Pubmed
Ramakrishnan,
A single residue in a novel ADP-ribosyl cyclase controls production of the calcium-mobilizing messengers cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate.
2010,
Pubmed
,
Echinobase
Ramos,
Calcium- and polyphosphate-containing acidic granules of sea urchin eggs are similar to acidocalcisomes, but are not the targets for NAADP.
2010,
Pubmed
,
Echinobase
Reimer,
Identification and partial characterization of yolk and cortical granule proteins in eggs and embryos of the starfish, Pisaster ochraceus.
1995,
Pubmed
,
Echinobase
Ruas,
Purified TPC isoforms form NAADP receptors with distinct roles for Ca(2+) signaling and endolysosomal trafficking.
2010,
Pubmed
,
Echinobase
Rybalchenko,
Membrane potential regulates nicotinic acid adenine dinucleotide phosphate (NAADP) dependence of the pH- and Ca2+-sensitive organellar two-pore channel TPC1.
2012,
Pubmed
Santella,
Nicotinic acid adenine dinucleotide phosphate-induced Ca(2+) release. Interactions among distinct Ca(2+) mobilizing mechanisms in starfish oocytes.
2000,
Pubmed
,
Echinobase
Schieder,
Characterization of two-pore channel 2 (TPCN2)-mediated Ca2+ currents in isolated lysosomes.
2010,
Pubmed
Sánchez-Tusie,
Characterization of NAADP-mediated calcium signaling in human spermatozoa.
2014,
Pubmed
,
Echinobase
Terasaki,
Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.
1991,
Pubmed
,
Echinobase
Wang,
TPC proteins are phosphoinositide- activated sodium-selective ion channels in endosomes and lysosomes.
2012,
Pubmed
Wessel,
Use of sea stars to study basic reproductive processes.
2010,
Pubmed
,
Echinobase
Wessel,
Cortical granule-specific components are present within oocytes and accessory cells during sea urchin oogenesis.
1989,
Pubmed
,
Echinobase
Wessel,
The biology of cortical granules.
2001,
Pubmed
Whitaker,
Calcium at fertilization and in early development.
2006,
Pubmed
Zong,
The two-pore channel TPCN2 mediates NAADP-dependent Ca(2+)-release from lysosomal stores.
2009,
Pubmed
