ECB-ART-32969
Nucleic Acids Res
1985 May 24;1310:3439-59. doi: 10.1093/nar/13.10.3439.
Primary organization of nucleosomal core particles is invariable in repressed and active nuclei from animal, plant and yeast cells.
Bavykin SG
,
Usachenko SI
,
Lishanskaya AI
,
Shick VV
,
Belyavsky AV
,
Undritsov IM
,
Strokov AA
,
Zalenskaya IA
,
Mirzabekov AD
.
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A refined map for the linear arrangement of histones along DNA in nucleosomal core particles has been determined by DNA-protein crosslinking. On one strand of 145-bp core DNA, histones are aligned in the following order: (5'') H2B25,35-H455,65-H375,85,95/H488-H2B105,11 5-H2A118-H3135,145/H2A145 (3'') (the subscripts give approximate distance in nucleotides of the main histone contacts from the 5''-end). Hence, the histone tetramer (H3,H4)2 and two dimers (
H2A -H2B) are arranged on double-stranded core DNA in a symmetrical and rather autonomous way:
H2A /H3-(
H2A -H2B)-(H3,H4)2-(H2B-
H2A )-H3/
H2A . The primary organization was found to be very similar in core particles isolated from repressed nuclei of sea urchin
sperm and chicken erythrocytes, from active in replication and transcription nuclei of Drosophila embryos and yeast and from somatic cells of lily. These data show that (i) the core structure is highly conserved in evolution and (ii) the overall inactivation of chromatin does not affect the arrangement of histones along DNA and thus does not seem to be regulated on this level of the core structure.
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4011430
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PMC341251
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Nucleic Acids Res
Genes referenced:
h2a
LOC100887844
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