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Methods Mol Biol
2015 Jan 01;1189:283-97. doi: 10.1007/978-1-4939-1164-6_19.
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Primary cell cultures of regenerating holothurian tissues.
Bello SA
,
Abreu-Irizarry RJ
,
García-Arrarás JE
.
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The ability to culture different cell types is essential for answering many questions in developmental and regenerative biology. Studies in marine organisms, in particular echinoderms, have been limited by the lack of well-described cellular culture systems. Here we describe a cell culture system, for normal or regenerating holothurian cells, that allows cell characterization by immunohistochemistry and scanning electron microscopy. These cell cultures can now be used to perform multiple types of experiments in order to explore the cellular, biochemical, and genomic aspects of echinoderm regenerative properties.
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