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Mol Biol Cell
2003 May 01;145:1808-17. doi: 10.1091/mbc.e02-03-0163.
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Controlled damage in thick specimens by multiphoton excitation.
Galbraith JA
,
Terasaki M
.
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Controlled damage by light energy has been a valuable tool in studies of cell function. Here, we show that the Ti:Sapphire laser in a multiphoton microscope can be used to cause localized damage within unlabeled cells or tissues at greater depths than previously possible. We show that the damage is due to a multiphoton process and made wounds as small as 1 microm in diameter 20 microm from the surface. A characteristic fluorescent scar allows monitoring of the damage and identifies the wound site in later observations. We were able to lesion a single axon within a bundle of nerves, locally interrupt organelle transport within one axon, cut dendrites in a zebrafish embryo, ablate a mitotic pole in a sea urchin egg, and wound the plasma membrane and nuclear envelope in starfish oocytes. The starfish nucleus collapsed approximately 1 h after wounding, indicating that loss of compartmentation barrier makes the structure unstable; surprisingly, the oocyte still completed meiotic divisions when exposed to maturation hormone, indicating that the compartmentalization and translocation of cdk1 and its regulators is not required for this process. Multiphoton excitation provides a new means for producing controlled damage deep within tissues or living organisms.
Bargmann,
Laser killing of cells in Caenorhabditis elegans.
1995, Pubmed
Bargmann,
Laser killing of cells in Caenorhabditis elegans.
1995,
Pubmed
Berns,
Laser scissors and tweezers.
1998,
Pubmed
Bi,
Calcium-regulated exocytosis is required for cell membrane resealing.
1995,
Pubmed
,
Echinobase
Buchstaller,
Micro-scale chromophore-assisted laser inactivation of nerve growth cone proteins.
2000,
Pubmed
Denk,
Two-photon laser scanning fluorescence microscopy.
1990,
Pubmed
Galbraith,
Slow transport of unpolymerized tubulin and polymerized neurofilament in the squid giant axon.
1999,
Pubmed
Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
Heald,
Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase.
1993,
Pubmed
Hopt,
Highly nonlinear photodamage in two-photon fluorescence microscopy.
2001,
Pubmed
Jontes,
Growth cone and dendrite dynamics in zebrafish embryos: early events in synaptogenesis imaged in vivo.
2000,
Pubmed
Kalab,
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
2002,
Pubmed
Khodjakov,
Centrosomes enhance the fidelity of cytokinesis in vertebrates and are required for cell cycle progression.
2001,
Pubmed
Khodjakov,
A synergy of technologies: combining laser microsurgery with green fluorescent protein tagging.
1997,
Pubmed
Kishimoto,
Activation of MPF at meiosis reinitiation in starfish oocytes.
1999,
Pubmed
,
Echinobase
Koester,
Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage.
1999,
Pubmed
Liang,
Wavelength dependence of cell cloning efficiency after optical trapping.
1996,
Pubmed
Liu,
Laser ablations reveal functional relationships of segmental hindbrain neurons in zebrafish.
1999,
Pubmed
McNeil,
Patching plasma membrane disruptions with cytoplasmic membrane.
2000,
Pubmed
,
Echinobase
McNeil,
Loss, restoration, and maintenance of plasma membrane integrity.
1997,
Pubmed
Okano-Uchida,
In vivo regulation of cyclin A/Cdc2 and cyclin B/Cdc2 through meiotic and early cleavage cycles in starfish.
1998,
Pubmed
,
Echinobase
Ookata,
Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes.
1992,
Pubmed
,
Echinobase
Park,
Analysis of upstream elements in the HuC promoter leads to the establishment of transgenic zebrafish with fluorescent neurons.
2000,
Pubmed
Pines,
Cell cycle. Checkpoint on the nuclear frontier.
1999,
Pubmed
Pines,
Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport.
1991,
Pubmed
Reddy,
Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes.
2001,
Pubmed
Sluder,
The reproduction of centrosomes: nuclear versus cytoplasmic controls.
1986,
Pubmed
,
Echinobase
Steinhardt,
Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release.
1994,
Pubmed
,
Echinobase
Takizawa,
Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C.
2000,
Pubmed
Tamm,
Laser microbeam study of a rotary motor in termite flagellates. Evidence that the axostyle complex generates torque.
1978,
Pubmed
Terasaki,
Redistribution of cytoplasmic components during germinal vesicle breakdown in starfish oocytes.
1994,
Pubmed
,
Echinobase
Terasaki,
Large plasma membrane disruptions are rapidly resealed by Ca2+-dependent vesicle-vesicle fusion events.
1997,
Pubmed
,
Echinobase
Toyoshima-Morimoto,
Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase.
2001,
Pubmed
Walker,
Asymmetric behavior of severed microtubule ends after ultraviolet-microbeam irradiation of individual microtubules in vitro.
1989,
Pubmed
,
Echinobase