Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
EMBO J
1989 Oct 01;810:3053-8. doi: 10.1002/j.1460-2075.1989.tb08456.x.
Show Gene links
Show Anatomy links
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
Labbé JC
,
Capony JP
,
Caput D
,
Cavadore JC
,
Derancourt J
,
Kaghad M
,
Lelias JM
,
Picard A
,
Dorée M
.
???displayArticle.abstract???
We have purified to near homogeneity the M-phase-specific protein kinase from starfish oocytes at first meiotic metaphase, using an improved procedure based on affinity chromatography on the immobilized yeast protein suc1. As already reported, this is identical to MPF, the cytoplasmic factor that controls entry of eukaryotic cells into M-phase. MPF is a complex formed by the stoichiometric association of a 34-kd polypeptide previously identified as cdc2 with a polypeptide that migrates with the same mobility as starfish cyclin in SDS-PAGE (apparent mol. wt 47 kd). A cDNA clone encoding starfish cyclin B has been isolated and its sequence determined. It contains a single open reading frame encoding a predicted 43 729-dalton protein. Partial microsequencing of the 47-kd polypeptide component of MPF allowed its identification as the starfish cyclin. Since the apparent mol. wt of native starfish MPF was found to be less than 100 kd, it is a heterodimer comprising one molecule of cdc2 and one molecule of cyclin B.
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987, Pubmed
Aebersold,
Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
1987,
Pubmed
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Echinobase
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Brizuela,
p13suc1 acts in the fission yeast cell division cycle as a component of the p34cdc2 protein kinase.
1987,
Pubmed
Caput,
Identification of a common nucleotide sequence in the 3'-untranslated region of mRNA molecules specifying inflammatory mediators.
1986,
Pubmed
Cathala,
A method for isolation of intact, translationally active ribonucleic acid.
1983,
Pubmed
Draetta,
Identification of p34 and p13, human homologs of the cell cycle regulators of fission yeast encoded by cdc2+ and suc1+.
1987,
Pubmed
Draetta,
Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.
1989,
Pubmed
,
Echinobase
Evans,
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division.
1983,
Pubmed
,
Echinobase
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
Hayles,
The fission yeast cell cycle control gene cdc2: isolation of a sequence suc1 that suppresses cdc2 mutant function.
1986,
Pubmed
Labbe,
Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+.
1988,
Pubmed
,
Echinobase
Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
,
Echinobase
Labbé,
An M-phase-specific protein kinase of Xenopus oocytes: partial purification and possible mechanism of its periodic activation.
1988,
Pubmed
Lehner,
Expression and function of Drosophila cyclin A during embryonic cell cycle progression.
1989,
Pubmed
Lohka,
Purification of maturation-promoting factor, an intracellular regulator of early mitotic events.
1988,
Pubmed
Lohka,
Mitotic control by metaphase-promoting factor and cdc proteins.
1989,
Pubmed
Minshull,
Translation of cyclin mRNA is necessary for extracts of activated xenopus eggs to enter mitosis.
1989,
Pubmed
Picard,
Role of protein synthesis and proteases in production and inactivation of maturation-promoting activity during meiotic maturation of starfish oocytes.
1985,
Pubmed
,
Echinobase
Pines,
Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs.
1987,
Pubmed
,
Echinobase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schollmeyer,
Calpain II involvement in mitosis.
1988,
Pubmed
Shaw,
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
1986,
Pubmed
Shoji-Kasai,
Thiol protease-specific inhibitor E-64 arrests human epidermoid carcinoma A431 cells at mitotic metaphase.
1988,
Pubmed
Standart,
Cyclin synthesis, modification and destruction during meiotic maturation of the starfish oocyte.
1987,
Pubmed
,
Echinobase
Swenson,
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes.
1986,
Pubmed
Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
