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Molecules
2013 Jun 19;186:7179-93. doi: 10.3390/molecules18067179.
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Immunomodulatory effect of Stichopus japonicus acid mucopolysaccharide on experimental hepatocellular carcinoma in rats.
Song Y
,
Jin SJ
,
Cui LH
,
Ji XJ
,
Yang FG
.
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Stichopus japonicus acid mucopolysaccharide (SJAMP) is an important biologically active compound that can be extracted from the body wall of the sea cucumber. The present study investigated the anti-tumor and immunomodulatory effects of SJAMP in an experimental hepatocellular carcinoma (HCC) model in rats. Three doses of SJAMP (17.5 mg/kg, 35 mg/kg, and 70 mg/kg administered 5 days/week via oral gavage) were given to rats with diethylnitrosamine (DEN)-induced HCC. SJAMP treatment significantly inhibited DEN-induced HCC by reducing both the number and mean volume of nodules, decreasing serum a-fetoprotein (AFP) levels and proliferating cell nuclear antigen (PCNA) expression in liver, and increasing p21 expression. Furthermore, SJAMP decreased the serum levels of ALT, AST, GGT and TNF-α and increased serum IL-2. SJAMP administration also improved indices of spleen and thymus function and improved both macrophage phagocytosis and NK cell-mediated tumoricidal activity. Moreover, CD3+ and CD4+ T lymphocyte levels recovered significantly and the CD4+/CD8+ T cell ratio normalized in a dose-dependent manner. In conclusion, SJAMP effectively inhibited the growth of HCC through the stimulation of immune organs and tissue proliferation, leading to the enhancement of cellular immunity pathways in rats.
Figure 3. Representative photographs for immunohistochemical staining studies of PCNA and p21. (A) PCNA-stained cells (indicated with arrows) in normal control group, tumor control group and high-SJAMP-dose group (magnification factor × 200); (B) p21-stained cells (indicated with arrows) in normal control group, tumor control group and high-SJAMP-dose group (magnification factor × 400).
Figure 4. Examples of scatterplots presenting CD3+, CD4+ and CD8+ lymphocyte subsets. (A) Flow cytometric analysis of staining for CD3+ (x-axis) and CD4+ (y-axis) cells in the tumor control group. The CD3+ cells are represented in lower right quadrant, and the CD4+ cells are represented in the upper left quadrant. The CD3+CD4+ cells are represented in the upper right quadrant; (B) Flow cytometric analysis of staining for CD3+ (x-axis) and CD8+ (y-axis) cells in the tumor control group. The CD3+ cells are represented in the lower right quadrant, and the CD8+ cells are represented in the upper left quadrant. The CD3+CD8+ cells are represented in the upper right quadrant.
Amat,
Immunomodulatory effects of Abnormal Savda Munsiq, a traditional Uighur medicine, on the combined stress mice.
2009, Pubmed
Amat,
Immunomodulatory effects of Abnormal Savda Munsiq, a traditional Uighur medicine, on the combined stress mice.
2009,
Pubmed
Amin,
Saffron: a potential candidate for a novel anticancer drug against hepatocellular carcinoma.
2011,
Pubmed
Anderson,
Tumor necrosis factor-alpha in the pathogenesis and treatment of cancer.
2004,
Pubmed
Bordbar,
High-value components and bioactives from sea cucumbers for functional foods--a review.
2011,
Pubmed
,
Echinobase
Budhu,
Prediction of venous metastases, recurrence, and prognosis in hepatocellular carcinoma based on a unique immune response signature of the liver microenvironment.
2006,
Pubmed
Carl,
Autoreactive T cells escape clonal deletion in the thymus by a CD24-dependent pathway.
2008,
Pubmed
Chakraborty,
Cell proliferation and hepatocarcinogenesis in rat initiated by diethylnitrosamine and promoted by phenobarbital: potential roles of early DNA damage and liver metallothionein expression.
2007,
Pubmed
Chen,
Immunohistochemical analysis of pro-apoptotic Bid level in chronic hepatitis, hepatocellular carcinoma and liver metastases.
2001,
Pubmed
Chen,
Effect of paeonol on antioxidant and immune regulatory activity in hepatocellular carcinoma rats.
2012,
Pubmed
Fan,
[Studies on the purification of water-soluble holothurian glycosides from Apostichopus japonicus and their tumor suppressing activity].
2009,
Pubmed
,
Echinobase
Fan,
[A new method for separation and purification of the acidic mucopolysaccharide from Stichopus japonicus].
1982,
Pubmed
,
Echinobase
Gomaa,
Hepatocellular carcinoma: epidemiology, risk factors and pathogenesis.
2008,
Pubmed
Grivennikov,
Immunity, inflammation, and cancer.
2010,
Pubmed
Grzywacz,
NK cells become Ki-67+ in MLC and expand depending on the lack of ligand for KIR on stimulator cells in IL-2 supplemented MLC.
2002,
Pubmed
Haybaeck,
A lymphotoxin-driven pathway to hepatocellular carcinoma.
2009,
Pubmed
Kariya,
Structure of fucose branches in the glycosaminoglycan from the body wall of the sea cucumber Stichopus japonicus.
1997,
Pubmed
,
Echinobase
Kim,
Anti-cancer activity and mechanistic features of a NK cell activating molecule.
2009,
Pubmed
Kotoh,
Antiangiogenic therapy of human esophageal cancers with thalidomide in nude mice.
1999,
Pubmed
Liu,
The effect of Gd@C82(OH)22 nanoparticles on the release of Th1/Th2 cytokines and induction of TNF-alpha mediated cellular immunity.
2009,
Pubmed
Liu,
Effect of two selenium sources on hepatocarcinogenesis and several angiogenic cytokines in diethylnitrosamine-induced hepatocarcinoma rats.
2012,
Pubmed
Lu,
The research progress of antitumorous effectiveness of Stichopus japonicus acid mucopolysaccharide in north of China.
2009,
Pubmed
,
Echinobase
Lu,
The effects of Stichopus japonicus acid mucopolysaccharide on the apoptosis of the human hepatocellular carcinoma cell line HepG2.
2010,
Pubmed
,
Echinobase
Mebius,
Structure and function of the spleen.
2005,
Pubmed
Mocellin,
Tumor necrosis factor, cancer and anticancer therapy.
2005,
Pubmed
Nishimura,
Distinct role of antigen-specific T helper type 1 (Th1) and Th2 cells in tumor eradication in vivo.
1999,
Pubmed
Oflazoglu,
Macrophages contribute to the antitumor activity of the anti-CD30 antibody SGN-30.
2007,
Pubmed
Oyama,
Reduction of hepatocarcinogenesis by ursodeoxycholic acid in rats.
2002,
Pubmed
Pardee,
Immunotherapy of hepatocellular carcinoma: Unique challenges and clinical opportunities.
2012,
Pubmed
Rajewsky,
Liver carcinogenesis by diethylnitrosamine in the rat.
1966,
Pubmed
Shavit,
Effects of fentanyl on natural killer cell activity and on resistance to tumor metastasis in rats. Dose and timing study.
2004,
Pubmed
Tsai,
Analysis of host versus tumor interaction in cancer patients: opposing role of transforming growth factor-beta1 and interleukin-6 in the development of in situ tumor immunity.
2005,
Pubmed
Waga,
The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA.
1994,
Pubmed
Wang,
Regulatory T cells and Toll-like receptors in tumor immunity.
2006,
Pubmed
Yuan,
Immunomodulation and antitumor activity of kappa-carrageenan oligosaccharides.
2006,
Pubmed
Zerbini,
Radiofrequency thermal ablation for hepatocellular carcinoma stimulates autologous NK-cell response.
2010,
Pubmed
