Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Cell Biol
1989 Jun 01;96:2315-21. doi: 10.1128/mcb.9.6.2315-2321.1989.
Show Gene links
Show Anatomy links
An embryonic enhancer determines the temporal activation of a sea urchin late H1 gene.
Lai ZC
,
DeAngelo DJ
,
DiLiberto M
,
Childs G
.
???displayArticle.abstract???
Normal development requires that individual genes be expressed in their correct temporal patterns, but the mechanisms regulating this process during early embryogenesis are poorly understood. We have studied the early and late sea urchin histone genes during embryogenesis to address the molecular mechanisms controlling temporal gene expression. By measuring the changes in expression of cloned H1-beta DNA constructs after microinjection into fertilized one-cell zygotes, we demonstrated that a highly conserved 30-base-pair segment of DNA between positions -288 and -317 (USE IV) is responsible for the transcriptional activation of this late histone gene at the late blastula stage. In this report, we demonstrate that an oligonucleotide corresponding to USE IV acts as an embryonic enhancer element capable of activating the simian virus 40 early promoter in a stage-specific manner. Using an in vivo competition assay and in vitro DNase I footprinting and mobility shift assays, we also identified a protein(s) that interacts with this enhancer. Results of the competition assay suggested that this factor acts to stimulate transcription of the H1-beta gene. The factor was found to be stored in mature eggs as well as in all embryonic stages examined. The mobility of the factor found in eggs, however, differed from that of the embryonic form, which suggested that posttranslational modification occurs after fertilization.
Barberis,
Mutually exclusive interaction of the CCAAT-binding factor and of a displacement protein with overlapping sequences of a histone gene promoter.
1987, Pubmed,
Echinobase
Barberis,
Mutually exclusive interaction of the CCAAT-binding factor and of a displacement protein with overlapping sequences of a histone gene promoter.
1987,
Pubmed
,
Echinobase
Biggin,
Transcription factors that activate the Ultrabithorax promoter in developmentally staged extracts.
1988,
Pubmed
Calzone,
Developmental appearance of factors that bind specifically to cis-regulatory sequences of a gene expressed in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Carey,
Analysis of RNA polymerase III transcription complexes by gel filtration.
1986,
Pubmed
Childs,
Histone gene expression during sea urchin embryogenesis: isolation and characterization of early and late messenger RNAs of Strongylocentrotus purpuratus by gene-specific hybridization and template activity.
1979,
Pubmed
,
Echinobase
Childs,
Sea urchin (lytechinus pictus) late-stage histone H3 and H4 genes: characterization and mapping of a clustered but nontandemly linked multigene family.
1982,
Pubmed
,
Echinobase
Colin,
Closely linked early and late histone H2B genes are differentially expressed after microinjection into sea urchin zygotes.
1988,
Pubmed
,
Echinobase
Colin,
Rapid repetitive microinjection.
1986,
Pubmed
,
Echinobase
Dale,
A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
1985,
Pubmed
Davidson,
Lineage-specific gene expression in the sea urchin embryo.
1985,
Pubmed
,
Echinobase
Flytzanis,
Ontogenic activation of a fusion gene introduced into sea urchin eggs.
1987,
Pubmed
,
Echinobase
Flytzanis,
Persistence and integration of cloned DNA in postembryonic sea urchins.
1985,
Pubmed
,
Echinobase
Flytzanis,
Developmental patterns of cytoplasmic transcript prevalence in sea urchin embryos.
1982,
Pubmed
,
Echinobase
Fried,
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
1981,
Pubmed
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Garner,
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
1981,
Pubmed
Gorman,
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
1982,
Pubmed
Heberlein,
Temporal pattern of alcohol dehydrogenase gene transcription reproduced by Drosophila stage-specific embryonic extracts.
1988,
Pubmed
Hieter,
Histone gene switch in the sea urchin embryo. Identification of late embryonic histone messenger ribonucleic acids and the control of their synthesis.
1979,
Pubmed
,
Echinobase
Hough-Evans,
Correct cell-type-specific expression of a fusion gene injected into sea urchin eggs.
1987,
Pubmed
,
Echinobase
Imperiale,
An enhancer-like element in the adenovirus E2 promoter contains sequences essential for uninduced and E1A-induced transcription.
1985,
Pubmed
Ito,
Synthesis and turnover of late H2B histone mRNA in developing embryos of the sea urchin, Strongylocentrotus purpuratus.
1988,
Pubmed
,
Echinobase
Jackson,
O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation.
1988,
Pubmed
Katula,
Ontogenic expression of a CyI actin fusion gene injected into sea urchin eggs.
1987,
Pubmed
,
Echinobase
Kemler,
Characterization of two nonallelic pairs of late histone H2A and H2B genes of the sea urchin: differential regulation in the embryo and tissue-specific expression in the adult.
1986,
Pubmed
,
Echinobase
Khoury,
Enhancer elements.
1983,
Pubmed
Knowles,
Isolation, characterization, and expression of the gene encoding the late histone subtype H1-gamma of the sea urchin Strongylocentrotus purpuratus.
1987,
Pubmed
,
Echinobase
Knowles,
Comparison of the late H1 histone genes of the sea urchins Lytechinus pictus and Strongelocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Knowles,
Temporal expression of late histone messenger RNA in the sea urchin Lytechinus pictus.
1984,
Pubmed
,
Echinobase
Krieg,
An enhancer responsible for activating transcription at the midblastula transition in Xenopus development.
1987,
Pubmed
Lai,
Both basal and ontogenic promoter elements affect the timing and level of expression of a sea urchin H1 gene during early embryogenesis.
1988,
Pubmed
,
Echinobase
Lai,
Characterization of the structure and transcriptional patterns of the gene encoding the late histone subtype H1-beta of the sea urchin Strongylocentrotus purpuratus.
1988,
Pubmed
,
Echinobase
Lieber,
Analysis of histone gene expression in adult tissues of the sea urchins Strongylocentrotus purpuratus and Lytechinus pictus: tissue-specific expression of sperm histone genes.
1986,
Pubmed
,
Echinobase
Livant,
An in vivo titration of regulatory factors required for expression of a fusion gene in transgenic sea urchin embryos.
1988,
Pubmed
,
Echinobase
Maxson,
Distinct organizations and patterns of expression of early and late histone gene sets in the sea urchin.
1983,
Pubmed
,
Echinobase
Maxson,
Expression and organization of histone genes.
1983,
Pubmed
,
Echinobase
McMahon,
Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis.
1985,
Pubmed
,
Echinobase
McMahon,
Inducible expression of a cloned heat shock fusion gene in sea urchin embryos.
1984,
Pubmed
,
Echinobase
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Ricci,
Oscillatory singular integrals and harmonic analysis on nilpotent groups.
1986,
Pubmed
Schaffner,
Redundancy of information in enhancers as a principle of mammalian transcription control.
1988,
Pubmed
Schirm,
The SV40 enhancer can be dissected into multiple segments, each with a different cell type specificity.
1987,
Pubmed
Sorger,
Heat shock factor is regulated differently in yeast and HeLa cells.
1987,
Pubmed
Vitelli,
Developmental regulation of micro-injected histone genes in sea urchin embryos.
1988,
Pubmed
,
Echinobase
Wilson,
Tissue-specific expression of actin genes injected into Xenopus embryos.
1986,
Pubmed
Wirth,
An octamer oligonucleotide upstream of a TATA motif is sufficient for lymphoid-specific promoter activity.
1987,
Pubmed
