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A calcium-binding, asparagine-linked oligosaccharide is involved in skeleton formation in the sea urchin embryo.
Farach-Carson MC
,
Carson DD
,
Collier JL
,
Lennarz WJ
,
Park HR
,
Wright GC
.
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We have previously identified a 130-kD cell surface protein that is involved in calcium uptake and skeleton formation by gastrula stage embryos of the sea urchin Strongylocentrotus purpuratus (Carson et al., 1985. Cell. 41:639-648). A monoclonal antibody designated mAb 1223 specifically recognizes the 130-kD protein and inhibits Ca+2 uptake and growth of the CaCO3 spicules produced by embryonic primary mesenchyme cells cultured in vitro. In this report, we demonstrate that the epitope recognized by mAb 1223 is located on an anionic, asparagine-linked oligosaccharide chain on the 130-kD protein. Combined enzymatic and chemical treatments indicate that the 1223 oligosaccharide contains fucose and sialic acid that is likely to be O-acetylated. Moreover, we show that the oligosaccharide chain containing the 1223 epitope specifically binds divalent cations, including Ca+2. We propose that one function of this negatively charged oligosaccharide moiety on the surfaces of primary mesenchyme cells is to facilitate binding and sequestration of Ca+2 ions from the blastocoelic fluid before internalization and subsequent deposition into the growing CaCO3 skeleton.
ACKERS,
DETERMINATION OF STOICHIOMETRY AND EQUILIBRIUM CONSTANTS FOR REVERSIBLY ASSOCIATING SYSTEMS BY MOLECULAR SIEVE CHROMATOGRAPHY.
1965, Pubmed
ACKERS,
DETERMINATION OF STOICHIOMETRY AND EQUILIBRIUM CONSTANTS FOR REVERSIBLY ASSOCIATING SYSTEMS BY MOLECULAR SIEVE CHROMATOGRAPHY.
1965,
Pubmed
Anstrom,
Localization and expression of msp130, a primary mesenchyme lineage-specific cell surface protein in the sea urchin embryo.
1987,
Pubmed
,
Echinobase
Ashwell,
Carbohydrate-specific receptors of the liver.
1982,
Pubmed
Ayotte,
N.m.r. spectroscopic observations related to the function of sulfate groups in heparin. Calcium binding vs. biological activity.
1986,
Pubmed
Caffrey,
A monoclonal antibody specifically modulates dihydropyridine-sensitive calcium current in BC3H1 myocytes.
1988,
Pubmed
Carlsen,
Purification and properties of an alpha-L-fucosidase from rat epididymis.
1972,
Pubmed
Carson,
A monoclonal antibody inhibits calcium accumulation and skeleton formation in cultured embryonic cells of the sea urchin.
1985,
Pubmed
,
Echinobase
Cheresh,
O-acetylation of disialoganglioside GD3 by human melanoma cells creates a unique antigenic determinant.
1984,
Pubmed
Decker,
Characterization of sea urchin primary mesenchyme cells and spicules during biomineralization in vitro.
1987,
Pubmed
,
Echinobase
Farach,
Developmental expression of a cell-surface protein involved in calcium uptake and skeleton formation in sea urchin embryos.
1987,
Pubmed
,
Echinobase
Farach,
Differential effects of p-nitrophenyl-D-xylosides on mouse blastocysts and uterine epithelial cells.
1988,
Pubmed
Feizi,
Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens.
1985,
Pubmed
Fischer,
Phosphomannosyl-enzyme receptors in rat liver. Subcellular distribution and role in intracellular transport of lysosomal enzymes.
1980,
Pubmed
Grant,
Developmental expression of cell-surface (glyco)proteins involved in gastrulation and spicule formation in sea urchin embryos.
1985,
Pubmed
,
Echinobase
HUMMEL,
Measurement of protein-binding phenomena by gel filtration.
1962,
Pubmed
Hart,
Effects of tunicamycin on the biosynthesis of glycosaminoglycans by embryonic chick cornea.
1978,
Pubmed
Heifetz,
Biosynthesis of N-glycosidically linked glycoproteins during gastrulation of sea urchin embryos.
1979,
Pubmed
,
Echinobase
Jaques,
Sialic acid. A calcium-binding carbohydrate.
1977,
Pubmed
Kaplan,
Phosphohexosyl components of a lysosomal enzyme are recognized by pinocytosis receptors on human fibroblasts.
1977,
Pubmed
Kouvonen,
The role of sialic acid in the binding of calcium ions to intrinsic factor and its intestinal receptor.
1984,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Long,
The binding of calcium ions by erythrocytes and 'ghost' -cell membranes.
1971,
Pubmed
Maruyama,
Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis.
1984,
Pubmed
Mintz,
Spicule formation by cultured embryonic cells from the sea urchin.
1981,
Pubmed
,
Echinobase
Ogawa,
Calcium binding to calmodulin: effects of ionic strength, Mg2+, pH and temperature.
1984,
Pubmed
Rutishauser,
Specific alteration of NCAM-mediated cell adhesion by an endoneuraminidase.
1985,
Pubmed
Schauer,
Analysis of sialic acids.
1987,
Pubmed
Schneider,
The effect of tunicamycin, an inhibitor of protein glycosylation, on embryonic development in the sea urchin.
1978,
Pubmed
,
Echinobase
Spiro,
Structure of the O-glycosidically linked carbohydrate units of fetuin.
1974,
Pubmed
Swiedler,
Stable oligosaccharide microheterogeneity at individual glycosylation sites of a murine major histocompatibility antigen derived from a B-cell lymphoma.
1983,
Pubmed
Takasaki,
Hydrazinolysis of asparagine-linked sugar chains to produce free oligosaccharides.
1982,
Pubmed
Ullrich,
Evidence for lysosomal enzyme recognition by human fibroblasts via a phosphorylated carbohydrate moiety.
1978,
Pubmed
Vannucchi,
Surface glycosaminoglycans and calcium distribution in 3T3 cells.
1978,
Pubmed
Varki,
The release and purification of sialic acids from glycoconjugates: methods to minimize the loss and migration of O-acetyl groups.
1984,
Pubmed
Wessel,
Ontogeny of the basal lamina in the sea urchin embryo.
1984,
Pubmed
,
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