Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Sci Signal
2014 Nov 18;7352:ra109. doi: 10.1126/scisignal.2005450.
Show Gene links
Show Anatomy links
Two-pore channels provide insight into the evolution of voltage-gated Ca2+ and Na+ channels.
Rahman T
,
Cai X
,
Brailoiu GC
,
Abood ME
,
Brailoiu E
,
Patel S
.
???displayArticle.abstract???
Four-domain voltage-gated Ca(2+) and Na(+) channels (CaV, NaV) underpin nervous system function and likely emerged upon intragenic duplication of a primordial two-domain precursor. To investigate if two-pore channels (TPCs) may represent an intermediate in this evolutionary transition, we performed molecular docking simulations with a homology model of TPC1, which suggested that the pore region could bind antagonists of CaV or NaV. CaV or NaV antagonists blocked NAADP (nicotinic acid adenine dinucleotide phosphate)-evoked Ca(2+) signals in sea urchin egg preparations and in intact cells that overexpressed TPC1. By sequence analysis and inspection of the model, we predicted a noncanonical selectivity filter in animal TPCs in which the carbonyl groups of conserved asparagine residues are positioned to coordinate cations. In contrast, a distinct clade of TPCs [TPCR (for TPC-related)] in several unicellular species had ion selectivity filters with acidic residues more akin to CaV. TPCRs were predicted to interact strongly with CaV antagonists. Our data suggest that acquisition of a "blueprint" pharmacological profile and changes in ion selectivity within four-domain voltage-gated ion channels may have predated intragenic duplication of an ancient two-domain ancestor.
???displayArticle.pubmedLink???
25406377
???displayArticle.pmcLink???PMC4327855 ???displayArticle.link???Sci Signal ???displayArticle.grants???[+]
Bazinet,
A gateway for phylogenetic analysis powered by grid computing featuring GARLI 2.0.
2014, Pubmed
Bazinet,
A gateway for phylogenetic analysis powered by grid computing featuring GARLI 2.0.
2014,
Pubmed
Brailoiu,
An ancestral deuterostome family of two-pore channels mediates nicotinic acid adenine dinucleotide phosphate-dependent calcium release from acidic organelles.
2010,
Pubmed
,
Echinobase
Brailoiu,
Essential requirement for two-pore channel 1 in NAADP-mediated calcium signaling.
2009,
Pubmed
Burnashev,
Control by asparagine residues of calcium permeability and magnesium blockade in the NMDA receptor.
1992,
Pubmed
Cai,
Ancient origin of four-domain voltage-gated Na+ channels predates the divergence of animals and fungi.
2012,
Pubmed
Cain,
Voltage-gated calcium channels and disease.
2011,
Pubmed
Calcraft,
NAADP mobilizes calcium from acidic organelles through two-pore channels.
2009,
Pubmed
Cang,
The voltage-gated sodium channel TPC1 confers endolysosomal excitability.
2014,
Pubmed
Catterall,
Activation of the action potential Na+ ionophore of cultured neuroblastoma cells by veratridine and batrachotoxin.
1975,
Pubmed
Catterall,
Receptor sites for Ca2+ channel antagonists.
1992,
Pubmed
Cavalier-Smith,
Phylogeny and evolution of apusomonadida (protozoa: apusozoa): new genera and species.
2010,
Pubmed
Churamani,
Domain assembly of NAADP-gated two-pore channels.
2012,
Pubmed
Churchill,
NAADP mobilizes Ca(2+) from reserve granules, lysosome-related organelles, in sea urchin eggs.
2002,
Pubmed
,
Echinobase
Clapham,
International Union of Pharmacology. L. Nomenclature and structure-function relationships of CatSper and two-pore channels.
2005,
Pubmed
Darriba,
ProtTest 3: fast selection of best-fit models of protein evolution.
2011,
Pubmed
Dickinson,
Modulation of NAADP (nicotinic acid-adenine dinucleotide phosphate) receptors by K+ ions: evidence for multiple NAADP receptor conformations.
2003,
Pubmed
,
Echinobase
Eswar,
Comparative protein structure modeling using MODELLER.
2007,
Pubmed
Genazzani,
Unique inactivation properties of NAADP-sensitive Ca2+ release.
1996,
Pubmed
,
Echinobase
Gur Barzilai,
Convergent evolution of sodium ion selectivity in metazoan neuronal signaling.
2012,
Pubmed
Heinemann,
Calcium channel characteristics conferred on the sodium channel by single mutations.
1992,
Pubmed
Hockerman,
Molecular determinants of high affinity phenylalkylamine block of L-type calcium channels in transmembrane segment IIIS6 and the pore region of the alpha1 subunit.
1997,
Pubmed
Hockerman,
Molecular determinants of drug binding and action on L-type calcium channels.
1997,
Pubmed
Hooper,
Membrane topology of NAADP-sensitive two-pore channels and their regulation by N-linked glycosylation.
2011,
Pubmed
Hooper,
NAADP on target.
2012,
Pubmed
,
Echinobase
Ishibashi,
Molecular cloning of a novel form (two-repeat) protein related to voltage-gated sodium and calcium channels.
2000,
Pubmed
Jha,
Convergent regulation of the lysosomal two-pore channel-2 by Mg²⁺, NAADP, PI(3,5)P₂ and multiple protein kinases.
2014,
Pubmed
Kim,
Protein structure prediction and analysis using the Robetta server.
2004,
Pubmed
King,
Choanoflagellates.
2005,
Pubmed
Lee,
A derivative of NADP mobilizes calcium stores insensitive to inositol trisphosphate and cyclic ADP-ribose.
1995,
Pubmed
,
Echinobase
Lee,
Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate (NAADP) as messengers for calcium mobilization.
2012,
Pubmed
Liebeskind,
Evolution of sodium channels predates the origin of nervous systems in animals.
2011,
Pubmed
Liebeskind,
Independent acquisition of sodium selectivity in bacterial and animal sodium channels.
2013,
Pubmed
Lin-Moshier,
Photoaffinity labeling of nicotinic acid adenine dinucleotide phosphate (NAADP) targets in mammalian cells.
2012,
Pubmed
,
Echinobase
McCusker,
Structure of a bacterial voltage-gated sodium channel pore reveals mechanisms of opening and closing.
2012,
Pubmed
O'Reilly,
Bisphenol A binds to the local anesthetic receptor site to block the human cardiac sodium channel.
2012,
Pubmed
Patel,
Acidic calcium stores open for business: expanding the potential for intracellular Ca2+ signaling.
2010,
Pubmed
Payandeh,
The crystal structure of a voltage-gated sodium channel.
2011,
Pubmed
Payandeh,
Crystal structure of a voltage-gated sodium channel in two potentially inactivated states.
2012,
Pubmed
Peiter,
The vacuolar Ca2+-activated channel TPC1 regulates germination and stomatal movement.
2005,
Pubmed
Pitt,
Reconstituted human TPC1 is a proton-permeable ion channel and is activated by NAADP or Ca2+.
2014,
Pubmed
Ren,
A prokaryotic voltage-gated sodium channel.
2001,
Pubmed
Rietdorf,
Two-pore channels form homo- and heterodimers.
2011,
Pubmed
Rodrigues,
KoBaMIN: a knowledge-based minimization web server for protein structure refinement.
2012,
Pubmed
Ryan,
Episodic neurological channelopathies.
2010,
Pubmed
Rybalchenko,
Membrane potential regulates nicotinic acid adenine dinucleotide phosphate (NAADP) dependence of the pH- and Ca2+-sensitive organellar two-pore channel TPC1.
2012,
Pubmed
Savio-Galimberti,
Voltage-gated sodium channels: biophysics, pharmacology, and related channelopathies.
2012,
Pubmed
Schieder,
Characterization of two-pore channel 2 (TPCN2)-mediated Ca2+ currents in isolated lysosomes.
2010,
Pubmed
Strong,
Molecular evolution of voltage-sensitive ion channel genes: on the origins of electrical excitability.
1993,
Pubmed
Tikhonov,
Structural model for dihydropyridine binding to L-type calcium channels.
2009,
Pubmed
Tikhonov,
Architecture and pore block of eukaryotic voltage-gated sodium channels in view of NavAb bacterial sodium channel structure.
2012,
Pubmed
Tugba Durlu-Kandilci,
TPC2 proteins mediate nicotinic acid adenine dinucleotide phosphate (NAADP)- and agonist-evoked contractions of smooth muscle.
2010,
Pubmed
Wang,
TPC proteins are phosphoinositide- activated sodium-selective ion channels in endosomes and lysosomes.
2012,
Pubmed
Yarov-Yarovoy,
Molecular determinants of voltage-dependent gating and binding of pore-blocking drugs in transmembrane segment IIIS6 of the Na(+) channel alpha subunit.
2001,
Pubmed
Yu,
Overview of molecular relationships in the voltage-gated ion channel superfamily.
2005,
Pubmed
Zhang,
Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel.
2012,
Pubmed
