Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Biochem J
2004 Jun 01;380Pt 2:449-54. doi: 10.1042/BJ20031754.
Show Gene links
Show Anatomy links
Determination of cellular nicotinic acid-adenine dinucleotide phosphate (NAADP) levels.
Churamani D
,
Carrey EA
,
Dickinson GD
,
Patel S
.
???displayArticle.abstract??? Nicotinic acid-adenine dinucleotide phosphate (NAADP) is fast emerging as a new intracellular Ca2+-mobilizing messenger. In sea urchin egg homogenates, binding of NAADP to its receptor is not readily reversible; hence, prior incubation with low concentrations of NAADP is more effective in inhibiting subsequent binding of radiolabelled NAADP than incubating the preparation with the two ligands simultaneously [Patel, Churchill and Galione (2000) Biochem. J. 352, 725-729]. We extend this finding to show that NAADP is more effective still in inhibiting the subsequent radioligand binding at lower homogenate concentrations, an effect again quite probably due to the non-reversible nature of the receptor-ligand interaction. Enhanced sensitivity of the preparation to NAADP afforded by simple manipulation of the experimental conditions has been applied to determine low levels of NAADP in acid extracts from human red blood cells, rat hepatocytes and Escherichia coli without interference from NADP breakdown. Our improved method for the quantification of NAADP should prove useful in the further assessment of its signalling role within cells.
Aarhus,
Activation and inactivation of Ca2+ release by NAADP+.
1996, Pubmed,
Echinobase
Aarhus,
Activation and inactivation of Ca2+ release by NAADP+.
1996,
Pubmed
,
Echinobase
Aarhus,
ADP-ribosyl cyclase and CD38 catalyze the synthesis of a calcium-mobilizing metabolite from NADP.
1995,
Pubmed
Berridge,
Calcium--a life and death signal.
1998,
Pubmed
Berridge,
The versatility and universality of calcium signalling.
2000,
Pubmed
Berridge,
Metabolism of the novel Ca2+-mobilizing messenger nicotinic acid-adenine dinucleotide phosphate via a 2'-specific Ca2+-dependent phosphatase.
2002,
Pubmed
Berridge,
Solubilization of receptors for the novel Ca2+-mobilizing messenger, nicotinic acid adenine dinucleotide phosphate.
2002,
Pubmed
,
Echinobase
Billington,
Characterization of NAADP(+) binding in sea urchin eggs.
2000,
Pubmed
,
Echinobase
Billington,
Nicotinic acid adenine dinucleotide phosphate (NAADP) is present at micromolar concentrations in sea urchin spermatozoa.
2002,
Pubmed
,
Echinobase
Boittin,
Nicotinic acid adenine dinucleotide phosphate mediates Ca2+ signals and contraction in arterial smooth muscle via a two-pool mechanism.
2002,
Pubmed
Brailoiu,
Modulation of spontaneous transmitter release from the frog neuromuscular junction by interacting intracellular Ca(2+) stores: critical role for nicotinic acid-adenine dinucleotide phosphate (NAADP).
2003,
Pubmed
Cancela,
Specific Ca2+ signaling evoked by cholecystokinin and acetylcholine: the roles of NAADP, cADPR, and IP3.
2001,
Pubmed
Carrey,
Origin and characteristics of an unusual pyridine nucleotide accumulating in erythrocytes: positive correlation with degree of renal failure.
2003,
Pubmed
Chini,
Nicotinate adenine dinucleotide phosphate (NAADP) triggers a specific calcium release system in sea urchin eggs.
1995,
Pubmed
,
Echinobase
Chini,
Nicotinate-adenine dinucleotide phosphate-induced Ca(2+)-release does not behave as a Ca(2+)-induced Ca(2+)-release system.
1996,
Pubmed
,
Echinobase
Chini,
CD38 is the major enzyme responsible for synthesis of nicotinic acid-adenine dinucleotide phosphate in mammalian tissues.
2002,
Pubmed
Chini,
Nicotinic acid adenine dinucleotide phosphate: a new intracellular second messenger?
2002,
Pubmed
,
Echinobase
Churchill,
NAADP mobilizes Ca(2+) from reserve granules, lysosome-related organelles, in sea urchin eggs.
2002,
Pubmed
,
Echinobase
Churchill,
Sperm deliver a new second messenger: NAADP.
2003,
Pubmed
,
Echinobase
Clapper,
Pyridine nucleotide metabolites stimulate calcium release from sea urchin egg microsomes desensitized to inositol trisphosphate.
1987,
Pubmed
,
Echinobase
Dickinson,
Modulation of NAADP (nicotinic acid-adenine dinucleotide phosphate) receptors by K+ ions: evidence for multiple NAADP receptor conformations.
2003,
Pubmed
,
Echinobase
Genazzani,
Nicotinic acid-adenine dinucleotide phosphate mobilizes Ca2+ from a thapsigargin-insensitive pool.
1996,
Pubmed
,
Echinobase
Genazzani,
Pharmacological properties of the Ca2+-release mechanism sensitive to NAADP in the sea urchin egg.
1997,
Pubmed
,
Echinobase
Genazzani,
NAADP: an atypical Ca2+-release messenger?
2002,
Pubmed
Genazzani,
Unique inactivation properties of NAADP-sensitive Ca2+ release.
1996,
Pubmed
,
Echinobase
Graeff,
A novel cycling assay for nicotinic acid-adenine dinucleotide phosphate with nanomolar sensitivity.
2002,
Pubmed
Guse,
Cyclic ADP-ribose (cADPR) and nicotinic acid adenine dinucleotide phosphate (NAADP): novel regulators of Ca2+-signaling and cell function.
2002,
Pubmed
Lee,
A derivative of NADP mobilizes calcium stores insensitive to inositol trisphosphate and cyclic ADP-ribose.
1995,
Pubmed
,
Echinobase
Lee,
Enzymatic functions and structures of CD38 and homologs.
2000,
Pubmed
Lee,
Physiological functions of cyclic ADP-ribose and NAADP as calcium messengers.
2001,
Pubmed
Masgrau,
NAADP: a new second messenger for glucose-induced Ca2+ responses in clonal pancreatic beta cells.
2003,
Pubmed
Meredith,
Rat hepatocytes prepared without collagenase: prolonged retention of differentiated characteristics in culture.
1988,
Pubmed
Mitchell,
Ryanodine receptor type I and nicotinic acid adenine dinucleotide phosphate receptors mediate Ca2+ release from insulin-containing vesicles in living pancreatic beta-cells (MIN6).
2003,
Pubmed
Miyakawa,
Ca(2+)-sensor region of IP(3) receptor controls intracellular Ca(2+) signaling.
2001,
Pubmed
Patel,
Molecular properties of inositol 1,4,5-trisphosphate receptors.
1999,
Pubmed
Patel,
Unique kinetics of nicotinic acid-adenine dinucleotide phosphate (NAADP) binding enhance the sensitivity of NAADP receptors for their ligand.
2000,
Pubmed
,
Echinobase
Patel,
Coordination of Ca2+ signalling by NAADP.
2001,
Pubmed
Patel,
NAADP on the up in pancreatic beta cells-a sweet message?
2003,
Pubmed
Taylor,
Inositol trisphosphate receptors: Ca2+-modulated intracellular Ca2+ channels.
1998,
Pubmed
