Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1979 Oct 01;831:241-8. doi: 10.1083/jcb.83.1.241.
Show Gene links
Show Anatomy links
pH regulates the polymerization of actin in the sea urchin egg cortex.
Begg DA
,
Rebhun LI
.
???displayArticle.abstract???
The state of actin in the isolated cortex of the unfertilized sea urchin egg can be controlled by experimentally manipulating the pH of the isolation medium. Cortices isolated at the pH of the unfertilized egg (6.5--6.7) do not contain filamentous actin, while those isolated at the pH of the fertilized egg (7.3--7.5) develop large numbers of microvilli which contain bundles of actin filaments. Cortices that are isolated at pH 6.5 and then transferred to isolation medium buffered at pH 7.5 also develop actin filaments. However, the filaments are not arranged in bundles and microvilli do not form. Although the cortical granules in cortices isolated at pH 6.5 discharge at a free Ca++ concentration of approximately 10 micrometer, actin polymerization is not induced by increasing the Ca++ concentration of the isolation medium. These results suggest that the increase in cytoplasmic pH which occurs following fertilization induces the polymerization of actin in the egg cortex.
Begg,
The visualization of actin filament polarity in thin sections. Evidence for the uniform polarity of membrane-associated filaments.
1978, Pubmed,
Echinobase
Begg,
The visualization of actin filament polarity in thin sections. Evidence for the uniform polarity of membrane-associated filaments.
1978,
Pubmed
,
Echinobase
Behnke,
Electron microscopical observations on actinoid and myosinoid filaments in blood platelets.
1971,
Pubmed
Binder,
The in vitro assembly of flagellar outer doublet tubulin.
1978,
Pubmed
Burgess,
Polarized bundles of actin filaments within microvilli of fertilized sea urchin eggs.
1977,
Pubmed
,
Echinobase
Carlsson,
Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells.
1977,
Pubmed
Eddy,
Changes in the topography of the sea urchin egg after fertilization.
1976,
Pubmed
,
Echinobase
Epel,
Mechanisms of activation of sperm and egg during fertilization of sea urchin gametes.
1978,
Pubmed
,
Echinobase
Epel,
Methods for revoval of the vitelline membrane of sea urchin eggs. I. Use of dithiothreitol (Cleland Reagent).
1970,
Pubmed
,
Echinobase
Grainger,
Intracellular pH controls protein synthesis rate in the sea urchine egg and early embryo.
1979,
Pubmed
,
Echinobase
Johnson,
Intracellular pH and activation of sea urchin eggs after fertilisation.
1976,
Pubmed
,
Echinobase
Kasai,
Behavior of divalent cations and nucleotides bound to F-actin.
1969,
Pubmed
Mazia,
Turning on of activities in unfertilized sea urchin eggs: correlation with changes of the surface.
1975,
Pubmed
,
Echinobase
RAAFLAUB,
Applications of metal buffers and metal indicators in biochemistry.
1956,
Pubmed
Sanger,
Changing patterns of actin localization during cell division.
1975,
Pubmed
Schroeder,
Dynamics of the contractile ring.
1975,
Pubmed
Schroeder,
Microvilli on sea urchin eggs: a second burst of elongation.
1978,
Pubmed
,
Echinobase
Shen,
Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg.
1978,
Pubmed
,
Echinobase
Steinhardt,
Intracellular calcium release at fertilization in the sea urchin egg.
1977,
Pubmed
,
Echinobase
Stossel,
Interactions between actin, myosin, and an actin-binding protein from rabbit alveolar macrophages. Alveolar macrophage myosin Mg-2+-adenosine triphosphatase requires a cofactor for activation by actin.
1975,
Pubmed
Taylor,
The contractile basis of amoeboid movement. I. The chemical control of motility in isolated cytoplasm.
1973,
Pubmed
Taylor,
The contractile basis of ameboid movement. II. Structure and contractility of motile extracts and plasmalemma-ectoplasm ghosts.
1976,
Pubmed
Tilney,
Polymerization of actin. IV. Role of Ca++ and H+ in the assembly of actin and in membrane fusion in the acrosomal reaction of echinoderm sperm.
1978,
Pubmed
,
Echinobase
Tilney,
The polymerization of actin. III. Aggregates of nonfilamentous actin and its associated proteins: a storage form of actin.
1976,
Pubmed
Zucker,
Intracellular calcium release and the mechanisms of parthenogenetic activation of the sea urchin egg.
1978,
Pubmed
,
Echinobase
