ECB-ART-30183
Cell Differ
1979 Dec 01;86:431-5. doi: 10.1016/0045-6039(79)90039-3.
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Exogastrulation induced by heavy water in sea urchin larvae.
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Replacement of H2O with D2O in seawater causes exogastrulation in larvae of the sea urchins, Hemicentrotus pulcherrimus, Strongylocentrotus intermedius and S. nudus. When larvae at any stages before mesenchymal blastula stage are transferred to 40% D2O-seawater all of them develop gradually to exogastrulae and finally up to plutei with evaginated archenterons. Effects of D2O are partly reversible at limited steps of the way to exogastrulation. Fertilisation and cleavage are not affected appreciably by D2O (50% or less) except for the delay of cleavage.
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Genes referenced: LOC100887844