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Immunogenetics
2004 Oct 01;567:531-43. doi: 10.1007/s00251-004-0711-0.
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Constitutive expression and alternative splicing of the exons encoding SCRs in Sp152, the sea urchin homologue of complement factor B. Implications on the evolution of the Bf/C2 gene family.
Terwilliger DP
,
Clow LA
,
Gross PS
,
Smith LC
.
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The purple sea urchin, Strongylocentrotus purpuratus, possesses a non-adaptive immune system including elements homologous to C3 and factor B (Bf) of the vertebrate complement system. SpBf is composed of motifs typical of the Bf/C2 protein family. Expression of Sp152 (encodes SpBf) was identified in the phagocyte type of coelomocyte in addition to gut, pharynx and esophagus, which may have been due to the presence of these coelomocytes in and on all tissues of the animal. Sp152 expression in coelomocytes was constitutive and non-inducible based on comparisons between pre- and post-injection with lipopolysaccharide or sterile seawater. The pattern of five short consensus repeats (SCRs) in SpBf has been considered ancestral compared to other deuterostome Bf/C2 proteins that contain either three or four SCRs. Three alternatively spliced messages were identified for Sp152 and designated Sp152Delta1, Sp152Delta4, and Sp152Delta1+Delta4, based on which of the five SCRs were deleted. Sp152Delta4 had an in-frame deletion of SCR4, which would encode a putative SpBfDelta4 protein with four SCRs rather than five. On the other hand, both Sp152Delta1 and Sp152Delta1+Delta4 had a frame-shift that introduced a stop codon six amino acids downstream of the splice site for SCR1, and would encode putative proteins composed only of the leader. Comparisons between the full-length SpBf and its several splice variants with other Bf/C2 proteins suggested that the early evolution of this gene family may have involved a combination of gene duplications and deletions of exons encoding SCRs.
Al-Sharif,
Sea urchin coelomocytes specifically express a homologue of the complement component C3.
1998, Pubmed,
Echinobase
Al-Sharif,
Sea urchin coelomocytes specifically express a homologue of the complement component C3.
1998,
Pubmed
,
Echinobase
Azumi,
Genomic analysis of immunity in a Urochordate and the emergence of the vertebrate immune system: "waiting for Godot".
2003,
Pubmed
Baumann,
Hepatic acute phase reaction in vivo and in vitro.
1989,
Pubmed
Baumann,
The acute phase response.
1994,
Pubmed
Botto,
Biosynthesis and secretion of complement component (C3) by activated human polymorphonuclear leukocytes.
1992,
Pubmed
Burset,
Analysis of canonical and non-canonical splice sites in mammalian genomes.
2000,
Pubmed
Cheng,
Alternatively spliced transcripts of the human complement C2 gene.
1994,
Pubmed
Clow,
Expression of SpC3, the sea urchin complement component, in response to lipopolysaccharide.
2000,
Pubmed
,
Echinobase
Clow,
The sea urchin complement homologue, SpC3, functions as an opsonin.
2004,
Pubmed
,
Echinobase
Coffaro,
Immune response in the sea urchin Lytechinus pictus.
1977,
Pubmed
,
Echinobase
Cole,
Tissue-specific pretranslational regulation of complement production in human mononuclear phagocytes.
1985,
Pubmed
Colten,
Tissue-specific regulation of inflammation.
1992,
Pubmed
Colten,
Regulation of complement protein biosynthesis in mononuclear phagocytes.
1986,
Pubmed
Colten,
Expression of the MHC class III genes.
1984,
Pubmed
Fedorova,
Introns in gene evolution.
2003,
Pubmed
Friese,
FHL-1/reconectin and factor H: two human complement regulators which are encoded by the same gene are differently expressed and regulated.
1999,
Pubmed
Ganter,
Characterization of mononuclear-phagocyte terminal maturation by mRNA phenotyping using a set of cloned cDNA probes.
1989,
Pubmed
Garnier,
Constitutive expression of murine complement factor B gene is regulated by the interaction of its upstream promoter with hepatocyte nuclear factor 4.
1996,
Pubmed
Geserick,
Isoelectrofocusing in the study of the Bf system: existence of two common subtypes of the BfF allele.
1983,
Pubmed
Gongora,
Independent duplications of Bf and C3 complement genes in the zebrafish.
1998,
Pubmed
Gross,
SpC3, the complement homologue from the purple sea urchin, Strongylocentrotus purpuratus, is expressed in two subpopulations of the phagocytic coelomocytes.
2000,
Pubmed
,
Echinobase
Gross,
Echinoderm immunity and the evolution of the complement system.
1999,
Pubmed
,
Echinobase
Hildemann,
Transplantation reactions of tropical Australian echinoderms.
1972,
Pubmed
,
Echinobase
Hourcade,
Analysis of the short consensus repeats of human complement factor B by site-directed mutagenesis.
1995,
Pubmed
Huang,
Complement factor B gene regulation: synergistic effects of TNF-alpha and IFN-gamma in macrophages.
2002,
Pubmed
Ishikawa,
Murine complement C2 and factor B genomic and cDNA cloning reveals different mechanisms for multiple transcripts of C2 and B.
1990,
Pubmed
Jiang,
Kinetic modeling and mathematical analysis indicate that acute phase gene expression in Hep 3B cells is regulated by both transcriptional and posttranscriptional mechanisms.
1995,
Pubmed
Johnson,
The coelomic elements of sea urchins (Strongylocentrotus). 3. In vitro reaction to bacteria.
1969,
Pubmed
,
Echinobase
Karp,
Specific allograft reactivity in the sea star Dermasterias imbricata.
1976,
Pubmed
,
Echinobase
Kato,
Duplication of the MHC-linked Xenopus complement factor B gene.
1995,
Pubmed
Katz,
Synergism between gamma interferon and lipopolysaccharide for synthesis of factor B, but not C2, in human fibroblasts.
1988,
Pubmed
Krushkal,
Evolutionary relationships among proteins encoded by the regulator of complement activation gene cluster.
2000,
Pubmed
Lee,
Sea urchin actin gene subtypes. Gene number, linkage and evolution.
1984,
Pubmed
,
Echinobase
Lévi-Strauss,
Primary cultures of murine astrocytes produce C3 and factor B, two components of the alternative pathway of complement activation.
1987,
Pubmed
Matsushita,
Two forms of guinea pig factor B of the alternative complement pathway with different molecular weights.
1989,
Pubmed
Minta,
Synthesis of complement factor B by the human monocyte U-937 cell line. Augmentation by immunostimulatory agents.
1988,
Pubmed
Miyama,
Complement proteins and macrophages. II. The secretion of factor B by lipopolysaccharide-stimulated macrophages.
1980,
Pubmed
Multerer,
Two cDNAs from the purple sea urchin, Strongylocentrotus purpuratus, encoding mosaic proteins with domains found in factor H, factor I, and complement components C6 and C7.
2004,
Pubmed
,
Echinobase
Nakao,
Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio).
1998,
Pubmed
Nakao,
Diversity of complement factor B/C2 in the common carp (Cyprinus carpio): three isotypes of B/C2-A expressed in different tissues.
2002,
Pubmed
Nonaka,
Tissue-specific initiation of murine complement factor B mRNA transcription.
1989,
Pubmed
Pancer,
Dynamic expression of multiple scavenger receptor cysteine-rich genes in coelomocytes of the purple sea urchin.
2000,
Pubmed
,
Echinobase
Pancer,
Origins of immunity: transcription factors and homologues of effector genes of the vertebrate immune system expressed in sea urchin coelomocytes.
1999,
Pubmed
,
Echinobase
Pasch,
Synthesis of complement components C3 and factor B in human keratinocytes is differentially regulated by cytokines.
2000,
Pubmed
Patthy,
Modular assembly of genes and the evolution of new functions.
2003,
Pubmed
Patthy,
Genome evolution and the evolution of exon-shuffling--a review.
1999,
Pubmed
Peelman,
Cloning and sequencing of the porcine complement factor B.
1991,
Pubmed
Shah,
The gene encoding the sea urchin complement protein, SpC3, is expressed in embryos and can be upregulated by bacteria.
2003,
Pubmed
,
Echinobase
Smith,
Alternative splicing in the control of gene expression.
1989,
Pubmed
Smith,
The echinoid immune system and the phylogenetic occurrence of immune mechanisms in deuterostomes.
1992,
Pubmed
,
Echinobase
Smith,
Thioester function is conserved in SpC3, the sea urchin homologue of the complement component C3.
2002,
Pubmed
,
Echinobase
Smith,
Sea urchin genes expressed in activated coelomocytes are identified by expressed sequence tags. Complement homologues and other putative immune response genes suggest immune system homology within the deuterostomes.
1996,
Pubmed
,
Echinobase
Smith,
Coelomocytes express SpBf, a homologue of factor B, the second component in the sea urchin complement system.
1998,
Pubmed
,
Echinobase
Smith,
Complement systems in invertebrates. The ancient alternative and lectin pathways.
1999,
Pubmed
,
Echinobase
Smith,
Lipopolysaccharide activates the sea urchin immune system.
1995,
Pubmed
,
Echinobase
Smith,
Shark complement: an assessment.
1998,
Pubmed
Smith,
The ancestral complement system in sea urchins.
2001,
Pubmed
,
Echinobase
Strunk,
gamma-Interferon increases expression of class III complement genes C2 and factor B in human monocytes and in murine fibroblasts transfected with human C2 and factor B genes.
1985,
Pubmed
Strunk,
Pretranslational regulation of the synthesis of the third component of complement in human mononuclear phagocytes by the lipid A portion of lipopolysaccharide.
1985,
Pubmed
Sumiyoshi,
Biosynthesis and secretion of MHC class III gene products (complement C4 and factor B) in the exocrine pancreas.
1997,
Pubmed
Sundsmo,
Factor B, the complement alternative pathway serine proteinase, is a major constitutive protein synthesized and secreted by resident and elicited mouse macrophages.
1985,
Pubmed
Sunyer,
Cloning, structure, and function of two rainbow trout Bf molecules.
1998,
Pubmed
Sutton,
Regulation of the synthesis of the third component of complement and factor B in cord blood monocytes by lipopolysaccharide.
1986,
Pubmed
Thomas,
Expression of a complete and functional complement system by human neuronal cells in vitro.
2000,
Pubmed
Whitehead,
Molecular biology of the human and mouse MHC class III genes: phylogenetic conservation, genetics and regulation of expression.
1985,
Pubmed
Wu,
Cell-specific expression of the human complement protein factor B gene: evidence for the role of two distinct 5'-flanking elements.
1987,
Pubmed
Zipfel,
FHL-1/reconectin: a human complement and immune regulator with cell-adhesive function.
1999,
Pubmed
