Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
The molecular characterization and expression of heat shock protein 90 (Hsp90) and 26 (Hsp26) cDNAs in sea cucumber (Apostichopus japonicus).
Zhao H
,
Yang H
,
Zhao H
,
Chen M
,
Wang T
.
???displayArticle.abstract???
The heat shock proteins (HSPs) are a family of proteins whose expression is enhanced in response to environmental stressors. The Apostichopus japonicus hsp90 and hsp26 genes were cloned using expressed sequence tag and rapid amplification of cDNA ends techniques. The full-length cDNA of Aphsp90 and Aphsp26 contains 3,458 and 1,688 nucleotides encoding 720 and 236 amino acids, respectively. Multiple alignments indicated that the deduced amino acid sequences of ApHsp90 and ApHsp26 shared a high level of identity with Hsp90 and small SHPs (sHSPs) sequences of zebrafish, ant, acorn worms, etc., and shared identical structural features with Hsp90 and sHSPs. The expression profiles of these two genes under heat treatment were investigated by real-time quantitative PCR. It was found that the messenger RNA (mRNA) transcripts of the two A. japonicus genes varied among different tissues under normal conditions and heat shock, and that the mRNA expression of the two genes was higher in the intestine compared to other tissues. Heat shock significantly elevated the expression of Aphsp90 and Aphsp26 mRNA in a temperature- and time-dependent manner. The results indicate that Aphsp90 and Aphsp26 played important roles in mediating the environmental stress in A. japonicus.
Abele,
Temperature-dependence of mitochondrial function and production of reactive oxygen species in the intertidal mud clam Mya arenaria.
2002, Pubmed
Abele,
Temperature-dependence of mitochondrial function and production of reactive oxygen species in the intertidal mud clam Mya arenaria.
2002,
Pubmed
Altschul,
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
1997,
Pubmed
Bruce,
Oxidative injury rapidly activates the heat shock transcription factor but fails to increase levels of heat shock proteins.
1993,
Pubmed
Caplan,
Hsp90's secrets unfold: new insights from structural and functional studies.
1999,
Pubmed
Chang,
Stressed-out lobsters: crustacean hyperglycemic hormone and stress proteins.
2005,
Pubmed
Chen,
Cloning of an orange-spotted grouper Epinephelus coioides heat shock protein 90AB (HSP90AB) and characterization of its expression in response to nodavirus.
2010,
Pubmed
Choresh,
The mitochondrial 60-kDa heat shock protein in marine invertebrates: biochemical purification and molecular characterization.
2004,
Pubmed
Chow,
Increased light intensity induces heat shock protein Hsp60 in coral species.
2009,
Pubmed
Crack,
Functional analysis of a small heat shock/alpha-crystallin protein from Artemia franciscana. Oligomerization and thermotolerance.
2002,
Pubmed
Craig,
The heat shock response.
1985,
Pubmed
Feder,
Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology.
1999,
Pubmed
Georgopoulos,
Role of the major heat shock proteins as molecular chaperones.
1993,
Pubmed
Gupta,
Phylogenetic analysis of the 90 kD heat shock family of protein sequences and an examination of the relationship among animals, plants, and fungi species.
1995,
Pubmed
Heise,
Production of reactive oxygen species by isolated mitochondria of the Antarctic bivalve Laternula elliptica (King and Broderip) under heat stress.
2003,
Pubmed
Hensold,
DNA binding of heat shock factor to the heat shock element is insufficient for transcriptional activation in murine erythroleukemia cells.
1990,
Pubmed
Huang,
Identification of the small heat shock protein, HSP21, of shrimp Penaeus monodon and the gene expression of HSP21 is inactivated after white spot syndrome virus (WSSV) infection.
2008,
Pubmed
Krebs,
Deleterious consequences of Hsp70 overexpression in Drosophila melanogaster larvae.
1997,
Pubmed
Li,
Molecular cloning, mRNA expression, and characterization of HSP90 gene from Chinese mitten crab Eriocheir japonica sinensis.
2009,
Pubmed
Linder,
Molecular characterization of a novel, developmentally regulated small embryonic chaperone from Caenorhabditis elegans.
1996,
Pubmed
MacRae,
Structure and function of small heat shock/alpha-crystallin proteins: established concepts and emerging ideas.
2000,
Pubmed
Parsell,
The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.
1993,
Pubmed
Pearl,
Structure and mechanism of the Hsp90 molecular chaperone machinery.
2006,
Pubmed
Picard,
Heat-shock protein 90, a chaperone for folding and regulation.
2002,
Pubmed
Pinsino,
Coelomocytes and post-traumatic response in the common sea star Asterias rubens.
2007,
Pubmed
,
Echinobase
Reineke,
Identification and expression of a small heat shock protein in two lines of the endoparasitic wasp Venturia canescens.
2005,
Pubmed
Rosic,
Gene expression profiles of cytosolic heat shock proteins Hsp70 and Hsp90 from symbiotic dinoflagellates in response to thermal stress: possible implications for coral bleaching.
2011,
Pubmed
Rungrassamee,
Expression and distribution of three heat shock protein genes under heat shock stress and under exposure to Vibrio harveyi in Penaeus monodon.
2010,
Pubmed
Sanders,
Stress proteins in aquatic organisms: an environmental perspective.
1993,
Pubmed
Shirk,
alpha-Crystallin protein cognates in eggs of the moth, Plodia interpunctella: possible chaperones for the follicular epithelium yolk protein.
1998,
Pubmed
Soetaert,
Molecular impact of propiconazole on Daphnia magna using a reproduction-related cDNA array.
2006,
Pubmed
Tomanek,
The Heat-Shock Response: Its Variation, Regulation and Ecological Importance in Intertidal Gastropods (genus Tegula).
2002,
Pubmed
Wang,
Effects of acute temperature or salinity stress on the immune response in sea cucumber, Apostichopus japonicus.
2008,
Pubmed
,
Echinobase
Welch,
Mammalian stress response: cell physiology, structure/function of stress proteins, and implications for medicine and disease.
1992,
Pubmed
Wu,
Characterization of heat shock protein 90 in the shrimp Metapenaeus ensis: Evidence for its role in the regulation of vitellogenin synthesis.
2008,
Pubmed
Zhang,
Molecular characterization of heat shock protein 90, 70 and 70 cognate cDNAs and their expression patterns during thermal stress and pupal diapause in the corn earworm.
2010,
Pubmed
van Montfort,
Crystal structure and assembly of a eukaryotic small heat shock protein.
2001,
Pubmed
