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Echinobase
ECB-ART-35924
Proc Natl Acad Sci U S A 1994 Dec 06;9125:12322-6. doi: 10.1073/pnas.91.25.12322.
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Modulator factor-binding sequence of the sea urchin early histone H2A promoter acts as an enhancer element.

Palla F , Bonura C , Anello L , Di Gaetano L , Spinelli G .


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The sea urchin early H2A histone gene, like the other four members of the repeating units, is transiently expressed during very early development. To investigate the mechanisms underlying the faithful expression of the early H2A gene, we focused our attention on the modulator element. We showed by DNase I cleavage protection patterns that the modulator includes the upstream sequence element 1 (USE1) and mapped at nucleotides -137 to -108 in the early H2A gene promoter. Functional tests conducted by microinjection into sea urchin embryos then showed that the modulator element binds the transcriptional factor called modulator-binding factor 1 (MBF-1). We found in fact that coinjection of an excess of the MBF-1-binding site, either as the modulator or as the USE1, efficiently impaired the activity of the H2A promoter. An unexpected finding was the expression of the reporter gene from the early H2A promoter at the gastrula stage of embryonic development, when the early histone genes are transcriptionally silent. In addition, we also found that the modulator element was active at the gastrula stage. The potential enhancer activity of the modulator was tested by microinjecting several constructs containing single or multiple copies of the modulator element placed 5'' or 3'' to a thymidine kinase gene (tk) promoter in both sea urchin embryos and Xenopus laevis oocytes and determining the expression of a reporter chloramphenicol acetyltransferase gene under the control of the linked tk promoter. We found that an oligonucleotide bearing the MBF-1-binding site activates the expression of the reporter gene independently of the position and orientation. We conclude that the modulator binds the MBF-1 activator and that it is a transcriptional enhancer of the early H2A histone gene.

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Genes referenced: h2a LOC100887844 LOC115917902

References [+] :
Anello, Different micrococcal nuclease cleavage patterns characterize transcriptionally active and inactive sea-urchin histone genes. 1986, Pubmed, Echinobase