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J Biomed Res
2019 Apr 30;341:44-53. doi: 10.7555/JBR.33.20190014.
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Participation of EML6 in the regulation of oocyte meiotic progression in mice.
Yin H
,
Hou X
,
Zhang T
,
Shi L
,
Su YQ
.
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The generation of a high-quality egg for reproduction requires faithful segregation of chromosome during oocyte meiosis. Here, we report that echinoderm microtubule-associated protein like 6 (EML6) is highly expressed in oocytes, and responsible for accurate segregation of homologous chromosomes in mice. Quantitative real-time RT-PCR and immunohistochemistry analyses revealed that EML6 was predominantly expressed by oocytes in the ovaries. Whole mount immunofluorescent staining showed that EML6 was colocalized with spindle microtubules in oocytes at various stages after meiotic resumption. This specialized localization was disrupted by nocodazole, the microtubule destabilizer, while enhanced by Taxol, a microtubule stabilizing reagent. In vivo knockdown of Eml6 expression by the specific siRNA resulted in chromosome misalignment and alteration in spindle dimension at both metaphase Ⅰ and Ⅱ stages, as well as the increased aneuploidy in the mature oocytes. Thus, these data suggest that EML family proteins participate in the control of oocyte meiotic division.
Baumann,
Error-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin.
2017, Pubmed
Baumann,
Error-prone meiotic division and subfertility in mice with oocyte-conditional knockdown of pericentrin.
2017,
Pubmed
Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
Bizzotto,
Eml1 loss impairs apical progenitor spindle length and soma shape in the developing cerebral cortex.
2017,
Pubmed
Cao,
Interference with the C-terminal structure of MARF1 causes defective oocyte meiotic division and female infertility in mice.
2018,
Pubmed
Chen,
EML4 promotes the loading of NUDC to the spindle for mitotic progression.
2015,
Pubmed
,
Echinobase
Conti,
Acquisition of oocyte competence to develop as an embryo: integrated nuclear and cytoplasmic events.
2018,
Pubmed
Eichenmuller,
The human EMAP-like protein-70 (ELP70) is a microtubule destabilizer that localizes to the mitotic apparatus.
2002,
Pubmed
,
Echinobase
Hamill,
Purification of a WD repeat protein, EMAP, that promotes microtubule dynamics through an inhibition of rescue.
1998,
Pubmed
,
Echinobase
Heald,
Microtubule dynamics.
2002,
Pubmed
Houtman,
Echinoderm microtubule-associated protein like protein 4, a member of the echinoderm microtubule-associated protein family, stabilizes microtubules.
2007,
Pubmed
,
Echinobase
Huttlin,
A tissue-specific atlas of mouse protein phosphorylation and expression.
2010,
Pubmed
Jin,
Identification of a mouse cytoskeleton-associated protein, CKAP2, with microtubule-stabilizing properties.
2004,
Pubmed
Joly,
Microtubule-severing activity of the AAA+ ATPase Katanin is essential for female meiotic spindle assembly.
2016,
Pubmed
Jones,
Molecular causes of aneuploidy in mammalian eggs.
2013,
Pubmed
Kalcheva,
Molecular and functional characteristics of MAP-2a: ability of MAP-2a versus MAP-2b to induce stable microtubules in COS cells.
1998,
Pubmed
Kielar,
Mutations in Eml1 lead to ectopic progenitors and neuronal heterotopia in mouse and human.
2014,
Pubmed
Kline-Smith,
The microtubule-destabilizing kinesin XKCM1 regulates microtubule dynamic instability in cells.
2002,
Pubmed
Lamb,
Effect of meiotic recombination on the production of aneuploid gametes in humans.
2005,
Pubmed
O'Connor,
Eml5, a novel WD40 domain protein expressed in rat brain.
2004,
Pubmed
,
Echinobase
Petry,
Mechanisms of Mitotic Spindle Assembly.
2016,
Pubmed
Pollmann,
Human EML4, a novel member of the EMAP family, is essential for microtubule formation.
2006,
Pubmed
Stern,
Single-molecule imaging of Tau dynamics on the microtubule surface.
2017,
Pubmed
Suprenant,
EMAP, an echinoderm microtubule-associated protein found in microtubule-ribosome complexes.
1993,
Pubmed
,
Echinobase
Tegha-Dunghu,
EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase.
2008,
Pubmed
Villén,
Large-scale phosphorylation analysis of mouse liver.
2007,
Pubmed