Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Cell Biol
1981 Dec 01;112:1125-37. doi: 10.1128/mcb.1.12.1125-1137.1981.
Show Gene links
Show Anatomy links
Sequence heterogeneity, multiplicity, and genomic organization of alpha- and beta-tubulin genes in sea urchins.
Alexandraki D
,
Ruderman JV
.
???displayArticle.abstract???
We analyzed the multiplicity, heterogeneity, and organization of the genes encoding the alpha and beta tubulins in the sea urchin Lytechinus pictus by using cloned complementary deoxyribonucleic acid (cDNA) and genomic tubulin sequences. cDNA clones were constructed by using immature spermatogenic testis polyadenylic acid-containing ribonucleic acid as a template. alpha- and beta-tubulin clones were identified by hybrid selection and in vitro translation of the corresponding messenger ribonucleic acids, followed by immunoprecipitation and two-dimensional gel electrophoresis of the translation products. The alpha cDNA clone contains a sequence that encodes the 48 C-terminal amino acids of alpha tubulin and 104 base pairs of the 3'' nontranslated portion of the messenger ribonucleic acid. The beta cDNA insertion contains the coding sequence for the 100-C terminal amino acids of beta tubulin and 83 pairs of the 3'' noncoding sequence. Hybrid selections performed at different criteria demonstrated the presence of several heterogeneous, closely related tubulin messenger ribonucleic acids, suggesting the existence of heterogeneous alpha- and beta-tubulin genes. Hybridization analyses indicated that there are at least 9 to 13 sequences for each of the two tubulin gene families per haploid genome. Hybridization of the cDNA probes to both total genomic DNA and cloned germline DNA fragments gave no evidence for close physical linkage of alpha-tubulin genes with beta-tubulin genes at the DNA level. In contrast, these experiments indicated that some genes within the same family are clustered.
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972, Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Bibring,
Heterogeneity of the alpha subunit of tubulin and the variability of tubulin within a single organism.
1976,
Pubmed
,
Echinobase
Bibring,
Tubulin synthesis in sea urchin embryos: almost all tubulin of the first cleavage mitotic apparatus derives from the unfertilized egg.
1977,
Pubmed
,
Echinobase
Blattner,
Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
1977,
Pubmed
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Boyer,
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
1969,
Pubmed
Britten,
Analysis of repeating DNA sequences by reassociation.
1974,
Pubmed
,
Echinobase
Bryan,
Separate mRNAs code for tubulin subunits.
1978,
Pubmed
Cleveland,
Multiple alpha and beta tubulin genes represent unlinked and dispersed gene families.
1981,
Pubmed
Cleveland,
Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
1980,
Pubmed
,
Echinobase
Cleveland,
Isolation of separate mRNAs for alpha- and beta-tubulin and characterization of the corresponding in vitro translation products.
1978,
Pubmed
Clewell,
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
1972,
Pubmed
Efstratiadis,
Enzymatic in vitro synthesis of globin genes.
1976,
Pubmed
Feit,
Comparison of the isoelectric and molecular weight properties of tubulin subunits.
1977,
Pubmed
Fujiwara,
Simultaneous localization of myosin and tubulin in human tissue culture cells by double antibody staining.
1978,
Pubmed
,
Echinobase
Fulton,
Programmed synthesis of flagellar tubulin during cell differentiation in Naegleria.
1975,
Pubmed
Gilbert,
The nucleotide sequence of the lac operator.
1973,
Pubmed
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Kafatos,
Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
1979,
Pubmed
Kemphues,
Mutation in a structural gene for a beta-tubulin specific to testis in Drosophila melanogaster.
1979,
Pubmed
Kemphues,
Mutation in a testis-specific beta-tubulin in Drosophila: analysis of its effects on meiosis and map location of the gene.
1980,
Pubmed
Kessler,
Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent.
1976,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed
Lis,
A novel arrangement of tandemly repeated genes at a major heat shock site in D. melanogaster.
1978,
Pubmed
Maniatis,
The isolation of structural genes from libraries of eucaryotic DNA.
1978,
Pubmed
Maniatis,
Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.
1975,
Pubmed
Marotta,
Biosynthesis of heterogeneous forms of mammalian brain tubulin subunits by multiple messenger RNAs.
1979,
Pubmed
McDonell,
Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
1977,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed
Ricciardi,
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation.
1979,
Pubmed
Roychoudhury,
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
1976,
Pubmed
Ruderman,
A portion of all major classes of histone messenger RNA in amphibian oocytes is polyadenylated.
1978,
Pubmed
,
Echinobase
Sharp,
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
1973,
Pubmed
Sheir-Neiss,
Identification of a gene for beta-tubulin in Aspergillus nidulans.
1978,
Pubmed
Snyder,
Biochemistry and physiology of microtubules.
1976,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Stephens,
Microtubules: structure, chemistry, and function.
1976,
Pubmed
,
Echinobase
Stephens,
Major membrane protein differences in cilia and flagella: evidence for a membrane-associated tubulin.
1977,
Pubmed
Stephens,
Differential protein synthesis and utilization during cilia formation in sea urchin embryos.
1977,
Pubmed
,
Echinobase
Stephens,
Primary structural differences among tubulin subunits from flagella, cilia, and the cytoplasm.
1978,
Pubmed
,
Echinobase
Sánchez,
A dispersed multigene family encoding tubulin in Drosophila melanogaster.
1980,
Pubmed
Thireos,
Identification of chorion protein precursors and the mRNAs that encode them in Drosophila melanogaster.
1979,
Pubmed
Valenzuela,
Nucleotide and corresponding amino acid sequences encoded by alpha and beta tubulin mRNAs.
1981,
Pubmed
Villa-Komaroff,
A bacterial clone synthesizing proinsulin.
1978,
Pubmed
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Wensink,
A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
1974,
Pubmed
Witman,
Chlamydomonas flagella. II. The distribution of tubulins 1 and 2 in the outer doublet microtubules.
1972,
Pubmed
