Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Biol Rep
2014 Jan 01;414:2617-27. doi: 10.1007/s11033-014-3120-y.
Show Gene links
Show Anatomy links
Cloning and expression analysis of the gene encoding fibrinogen-like protein A, a novel regeneration-related protein from Apostichopus japonicus.
Wu Y
,
Yao F
,
Mei Y
,
Chu B
,
Cheng C
,
Liu Y
,
Li X
,
Zou X
,
Hou L
.
???displayArticle.abstract???
Fibrinogen-like protein A (FGLA), a member of the fibrinogen-related protein superfamily, exists in different tissues of vertebrates and invertebrates. FGLA plays crucial roles including innate immune response, blood clotting and regeneration. In this study, the fibrinogen-like protein A (fglA) was cloned from Apostichopus japonicus using rapid amplification of cDNA ends PCR techniques. The cDNA sequence of fglA is 1,524 bp with a 849 bp open reading frame encoding a polypeptide of 282 amino acids, with an N-terminal signal peptide and a conserved C-terminal domain. Bioinformatic analysis revealed that the predicted molecular weight of the whole protein is 31.9 kDa and it has an isoelectric point of 5.64. In-situ hybridization demonstrated that fglA is widely distributed in body wall, intestines, longitudinal muscles and respiratory tree. The expression levels of fglA during different regeneration stages in the body wall, intestine and respiratory trees were analyzed by real-time PCR. The expression of fglA gradually increased within 1 h in body wall, and reached a plateau before decreasing to the basal level. This indicates that fglA is associated with the regeneration of Apostichopus japonicus.
Brockes,
Amphibian limb regeneration: rebuilding a complex structure.
1997, Pubmed
Brockes,
Amphibian limb regeneration: rebuilding a complex structure.
1997,
Pubmed
Chai,
The molecular cloning and characteristics of a fibrinogen-related protein (TfFREP1) gene from roughskin sculpin (Trachidermus fasciatus).
2012,
Pubmed
Dolmatov IYu,
Regeneration of the aquapharyngeal complex in the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota).
1992,
Pubmed
,
Echinobase
El-Karef,
Deficiency of tenascin-C attenuates liver fibrosis in immune-mediated chronic hepatitis in mice.
2007,
Pubmed
Forsthoefel,
Stem cell-based growth, regeneration, and remodeling of the planarian intestine.
2011,
Pubmed
Gokudan,
Horseshoe crab acetyl group-recognizing lectins involved in innate immunity are structurally related to fibrinogen.
1999,
Pubmed
Hara,
Isolation and characterization of a novel liver-specific gene, hepassocin, upregulated during liver regeneration.
2000,
Pubmed
Hara,
Molecular cloning and functional expression analysis of a cDNA for human hepassocin, a liver-specific protein with hepatocyte mitogenic activity.
2001,
Pubmed
Husmann,
Growth factors in skeletal muscle regeneration.
1996,
Pubmed
Kim,
Hepatic expression, synthesis and secretion of a novel fibrinogen/angiopoietin-related protein that prevents endothelial-cell apoptosis.
2000,
Pubmed
Liu,
Fibrinogen-like protein 1, a hepatocyte derived protein is an acute phase reactant.
2008,
Pubmed
Lu,
Ficolins and the fibrinogen-like domain.
1998,
Pubmed
Lu,
Collectins and ficolins: sugar pattern recognition molecules of the mammalian innate immune system.
2002,
Pubmed
Mashanov,
Expression of Wnt9, TCTP, and Bmp1/Tll in sea cucumber visceral regeneration.
2012,
Pubmed
,
Echinobase
Ning,
Role of fibrinogen-like protein 2 prothrombinase/fibroleukin in experimental and human allograft rejection.
2005,
Pubmed
Procopio,
Angiopoietin-1 and -2 coiled coil domains mediate distinct homo-oligomerization patterns, but fibrinogen-like domains mediate ligand activity.
1999,
Pubmed
Rijken,
Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot.
2006,
Pubmed
Sahni,
Binding of basic fibroblast growth factor to fibrinogen and fibrin.
1998,
Pubmed
Sahni,
Vascular endothelial growth factor binds to fibrinogen and fibrin and stimulates endothelial cell proliferation.
2000,
Pubmed
Su,
Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy.
2011,
Pubmed
Sun,
Large scale gene expression profiling during intestine and body wall regeneration in the sea cucumber Apostichopus japonicus.
2011,
Pubmed
,
Echinobase
Sánchez Alvarado,
Regeneration in the metazoans: why does it happen?
2000,
Pubmed
Tsonis,
Regeneration in vertebrates.
2000,
Pubmed
Vickery,
Utilization of a novel deuterostome model for the study of regeneration genetics: molecular cloning of genes that are differentially expressed during early stages of larval sea star regeneration.
2001,
Pubmed
,
Echinobase
Wadman,
Scar prevention: the healing touch.
2005,
Pubmed
Weissman,
Stem cells: units of development, units of regeneration, and units in evolution.
2000,
Pubmed
Yamamoto,
Molecular cloning and initial characterization of a novel fibrinogen-related gene, HFREP-1.
1993,
Pubmed
Yan,
Cloning and characterization of a mouse liver-specific gene mfrep-1, up-regulated in liver regeneration.
2002,
Pubmed
Zaret,
Generation and regeneration of cells of the liver and pancreas.
2008,
Pubmed
Zhang,
Fibrinogen-bearing protein genes in the snail Biomphalaria glabrata: characterization of two novel genes and expression studies during ontogenesis and trematode infection.
2008,
Pubmed
