Cell 1976 Dec 01;94 Pt 1:597-604. doi: 10.1016/0092-8674(76)90042-8.

Methylated constituents of poly(A)- and poly(A)+ polyribosomal RNA of sea urchin embryos.

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We have examined the poly(A)- and poly(A)+ polyribosomal (Ps) RNAs of developing sea urchin embryos to see whether they contain the capped structures previously observed in the poly(A)+ cytoplasmic RNA (mRNA) of a variety of eucaryotic organisms. We have found that both classes of Ps RNA contain 7-methylguanosine linked to the RNA by a 5''-5'' triphosphate bridge. Capped structures derived from either class of Ps RNA appear to be of the type m7G5''ppp5''NmpNp, with only a single sugar-methylated nucleoside per cap, and we have estimated that both classes of Ps RNA are capped to the same extent. The 5'' terminal 2''-0-methylated nucleosides have been identified and quantitated. The relative amounts are Am = 78%, Gm = 14%, Cm = 5%, Um = 3% for poly(A)- Ps RNA, and Am = 78%, Gm = 15%, Cm = 5%, Um = 2% for poly (A)+ Ps RNA. In addition, both classes of Ps RNA contain 2-4 internal residues of N6-methyladenylic acid per molecule.

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Genes referenced: LOC100887844