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Enzyme Res
2011 Jan 01;2011:679624. doi: 10.4061/2011/679624.
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Amylolytic microorganism from são paulo zoo composting: isolation, identification, and amylase production.
Pascon RC
,
Bergamo RF
,
Spinelli RX
,
de Souza ED
,
Assis DM
,
Juliano L
,
Vallim MA
.
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Composting is a way of transforming the organic waste into fertilizer, minimizing the use of inorganic compounds that may contaminate the environment. This transformation is the result of the microorganism action, converting complex carbon sources into energy. Enzymes that are exported by the microorganisms to the surrounding environment mediate this process. The aiming of the present work is to prospect the compost produced by the organic composting unit (OCU) of the Fundação Parque Zoológico de São Paulo (FPZSP) to find novel starch hydrolyzing organisms (SHO) that secrete large amounts of amylases under harsh conditions, such as high temperature. We found five bacterial isolates that have amylolytic activity induced by soluble starch and 39°C temperature of growth. These bacterial strains were identified by MALDI-TOF (Matrix-assisted laser desorption/ionization-Time of Flight) analysis, a rapid and efficient methodology for microbe identification in large scale. Our results present amylolytic strains that belong to diverse taxonomic groups (Solibacillus silvestris, Arthrobacter arilaitensis, Isoptericola variabilis, and Acinetobacter calcoaceticus); some of them have never been associated with this kind of hydrolytic activity before. The information regarding enzyme induction will be important to optimize the production by the bacterial isolates, which may be a great value for biotechnological applications.
Figure 1. Growth pattern of five isolates over 26-hour period. (a) 30°C, (b) 39°C, and (c) 45°C. All growth curves were done in triplicates for all three temperatures, the bars represent standard deviation.
Figure 2. Kinetics of amylase activity in the culture supernatants of five isolates (a) URX291, (b) URX303, (c) URX350, (d) UED 641, and (e) UED644 in starch medium for 26 hours at 30 (diamonds), 39 (squares), 45°C (triangles), and in LB rich medium at 30°C (crosses). n = 9. Vertical lines represent error bars.
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