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Echinobase
ECB-ART-31884
Cell 1989 Mar 10;565:829-38. doi: 10.1016/0092-8674(89)90687-9.
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Cdc2 protein kinase is complexed with both cyclin A and B: evidence for proteolytic inactivation of MPF.

Draetta G , Luca F , Westendorf J , Brizuela L , Ruderman J , Beach D .


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In the clam, Spisula, two previously described proteins known as cyclin A and B display the unusual property of selective proteolytic degradation at the end of each mitosis. We show here that clam oocytes and embryos contain a cdc2 protein kinase. This protein kinase is a component of the M phase promoting factor (MPF) in frog eggs and the M phase-specific histone H1 kinase in starfish. Clam cdc2 is found in association with both cyclin A and B, probably not as a trimolecular association, but as separate cdc2/cyclin A and cdc2/cyclin B complexes. Clam cdc2 and the associated cyclins bind to p13suc1-Sepharose. The p13-bound complex, and also anti-cyclin A or B immunoprecipitates, each display cell cycle-dependent histone H1 kinase activity. We suggest that in addition to the cdc2 protein kinase, the cyclins are further components of the M phase promoting factor and that cyclin proteolysis provides the mechanism of MPF inactivation and thus exit from mitosis.

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Genes referenced: LOC115919910 LOC586799