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Mar Drugs
2021 Jan 06;191:. doi: 10.3390/md19010021.
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Spinochrome Identification and Quantification in Pacific Sea Urchin Shells, Coelomic Fluid and Eggs Using HPLC-DAD-MS.
Vasileva EA
,
Mishchenko NP
,
Tran VTT
,
Vo HMN
,
Fedoreyev SA
.
Abstract
The high-performance liquid chromatography method coupled with diode array and mass spectrometric detector (HPLC-DAD-MS) method for quinonoid pigment identification and quantification in sea urchin samples was developed and validated. The composition and quantitative ratio of the quinonoid pigments of the shells of 16 species of sea urchins, collected in the temperate (Sea of Japan) and tropical (South-China Sea) climatic zones of the Pacific Ocean over several years, were studied. The compositions of the quinonoid pigments of sea urchins Maretia planulata, Scaphechinus griseus, Laganum decagonale and Phyllacanthus imperialis were studied for the first time. A study of the composition of the quinonoid pigments of the coelomic fluid of ten species of sea urchins was conducted. The composition of quinonoid pigments of Echinarachnius parma jelly-like egg membrane, of Scaphechinus mirabilis developing embryos and pluteus, was reported for the first time. In the case of Scaphechinus mirabilis, we have shown that the compositions of pigment granules of the shell epidermis, coelomic fluid, egg membrane, developing embryos and pluteus are different, which should enable a fuller understanding of the functions of pigments at different stages of life.
Figure 1. Structures of quinonoid pigments of sea urchins.
Figure 2. (A) HPLC chromatogram of one of the fractions obtained after chromatographic separation of the S. mirabilis ethyl acetate extract containing spinochrome D, together with the chromatogram obtained from the mass spectrometric (MS) detector in selected ion monitoring (SIM) mode with the targeted mass m/z [M − H]– of 237. (B) Absorption spectrum of spinochrome D. (C) Absorption spectrum of the compound detected by SIM, with retention time 6.67 min.
Figure 3. Typical HPLC profiles of extracts of the CF of Pacific sea urchins.
Ageenko,
Pigment cell differentiation in sea urchin blastula-derived primary cell cultures.
2014,
Pubmed
,
Echinobase
Aminin,
1,4-Naphthoquinones: Some Biological Properties and Application.
2020,
Pubmed
Anderson,
Distribution of spinochrome pigments in echinoids.
1969,
Pubmed
,
Echinobase
Brasseur,
The Roles of Spinochromes in Four Shallow Water Tropical Sea Urchins and Their Potential as Bioactive Pharmacological Agents.
2017,
Pubmed
,
Echinobase
Brasseur,
Identification and quantification of spinochromes in body compartments of Echinometra mathaei's coloured types.
2018,
Pubmed
,
Echinobase
Brasseur,
Echinometra mathaei and its ectocommensal shrimps: the role of sea urchin spinochrome pigments in the symbiotic association.
2018,
Pubmed
,
Echinobase
Coates,
Echinochrome A Release by Red Spherule Cells Is an Iron-Withholding Strategy of Sea Urchin Innate Immunity.
2018,
Pubmed
,
Echinobase
Drozdov,
[Morphology of gametes in sea urchins from Peter the Great Bay, Sea of Japan].
2010,
Pubmed
,
Echinobase
Egea,
Morphological and genetic analyses reveal a cryptic species complex in the echinoid Echinocardium cordatum and rule out a stabilizing selection explanation.
2016,
Pubmed
,
Echinobase
Fedoreyev,
Antiviral and Antioxidant Properties of Echinochrome A.
2018,
Pubmed
,
Echinobase
Hibino,
The immune gene repertoire encoded in the purple sea urchin genome.
2006,
Pubmed
,
Echinobase
Hira,
Autofluorescence mediated red spherulocyte sorting provides insights into the source of spinochromes in sea urchins.
2020,
Pubmed
,
Echinobase
Hou,
Naphthoquinones of the spinochrome class: occurrence, isolation, biosynthesis and biomedical applications.
2018,
Pubmed
,
Echinobase
Jeong,
Echinochrome A protects mitochondrial function in cardiomyocytes against cardiotoxic drugs.
2014,
Pubmed
,
Echinobase
Kominami,
Process of pigment cell specification in the sand dollar, Scaphechinus mirabilis.
2002,
Pubmed
,
Echinobase
Lebedev,
Interaction of natural polyhydroxy-1,4-naphthoquinones with superoxide anion-radical.
1999,
Pubmed
Lebedev,
Iron chelators and free radical scavengers in naturally occurring polyhydroxylated 1,4-naphthoquinones.
2008,
Pubmed
Li,
Strongylocins, novel antimicrobial peptides from the green sea urchin, Strongylocentrotus droebachiensis.
2008,
Pubmed
,
Echinobase
Matranga,
Monitoring chemical and physical stress using sea urchin immune cells.
2005,
Pubmed
,
Echinobase
Matranga,
Cellular and biochemical responses to environmental and experimentally induced stress in sea urchin coelomocytes.
2000,
Pubmed
,
Echinobase
Mischenko,
Echinamines A and B, first aminated hydroxynaphthazarins from the sea urchin Scaphechinus mirabilis.
2005,
Pubmed
,
Echinobase
Mishchenko,
Antiviral Potential of Sea Urchin Aminated Spinochromes against Herpes Simplex Virus Type 1.
2020,
Pubmed
,
Echinobase
Mishchenko,
Isolation and Structure Determination of Echinochrome A Oxidative Degradation Products.
2020,
Pubmed
,
Echinobase
NISHIBORI,
Isolation of echinochrome A from the spines of the sea urchin, Stomopneustes variolaris (Lamarck).
1961,
Pubmed
,
Echinobase
Shikov,
The offline combination of thin-layer chromatography and high-performance liquid chromatography with diode array detection and micrOTOF-Q mass spectrometry for the separation and identification of spinochromes from sea urchin (Strongylocentrotus droebachiensis) shells.
2011,
Pubmed
,
Echinobase
Smith,
Sensitization and histamine release by cells of the sand dollar, Mellita quinquiesperforata.
1985,
Pubmed
,
Echinobase
Takata,
Behavior of pigment cells closely correlates the manner of gastrulation in sea urchin embryos.
2004,
Pubmed
,
Echinobase
Vasileva,
New Aminonaphthoquinone from the Sea Urchins Strongylocentrotus pallidus and Mesocentrotus nudus.
2016,
Pubmed
,
Echinobase
Vasileva,
Diversity of Polyhydroxynaphthoquinone Pigments in North Pacific Sea Urchins.
2017,
Pubmed
,
Echinobase
Ziegenhorn,
Best Dressed Test: A Study of the Covering Behavior of the Collector Urchin Tripneustes gratilla.
2016,
Pubmed
,
Echinobase
