ECB-ART-47961
Stain Technol
1975 Sep 01;505:351-4. doi: 10.3109/10520297509117086.
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A decalification method for ultrastructure of echinoderm tissues.
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Decalcification of echinoderm tissues for electron microscopy can be achieved after glutaraldehyde-osmium fixation by treatment with a 1:1 mixture of 2% ascorbic acid and 0.3 M NaCl for 12-24 hours. Electron photomicrographs of material decalcified by this procedure are superior to those from EDTA-treated tissue and show few of the deleteriour effects produced by EDTA.
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