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Int J Mol Sci
2016 Feb 04;172:150. doi: 10.3390/ijms17020150.
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Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics.
Xu D
,
Sun L
,
Liu S
,
Zhang L
,
Yang H
.
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The sea cucumber Apostichopus japonicus is exploited as a commercial species owing to their high nutritive and medicinal value. Recent high summer temperatures have caused high mortality rates in A. japonicus. In this study, we applied the isobaric tag for relative and absolute quantitation (iTRAQ) technique to investigate the global protein expression profile under an acute short-term (48 h) heat stress. In total, 3432 proteins were identified, and 127 proteins showed significant heat stress responses, with 61 upregulated proteins and 66 downregulated proteins. Our results suggest that heat stress influenced the expression of proteins involved in various biological processes, such as tissue protection and detoxification, lipid and amino acid metabolism, energy production and usage, transcription and translation, cell apoptosis, and cell proliferation. These findings provide a better understanding about the response and thermo-tolerance mechanisms of A. japonicus under heat stress.
Figure 2. Enriched Gene Ontology (GO) analysis of differentially expressed proteins in the intestine under heat stress. The top 10 enriched GO-terms by cluster frequency in “Cellular component”, “Molecular function” and “Biological process” were shown, respectively.
Adams,
Proteomic responses of sea urchin embryos to stressful ultraviolet radiation.
2012, Pubmed,
Echinobase
Adams,
Proteomic responses of sea urchin embryos to stressful ultraviolet radiation.
2012,
Pubmed
,
Echinobase
Alp,
Activities of citrate synthase and NAD+-linked and NADP+-linked isocitrate dehydrogenase in muscle from vertebrates and invertebrates.
1976,
Pubmed
Bahl,
In vitro and in vivo suppression of gluconeogenesis by inhibition of pyruvate carboxylase.
1997,
Pubmed
Bahnson,
Structural mechanism of enoyl-CoA hydratase: three atoms from a single water are added in either an E1cb stepwise or concerted fashion.
2002,
Pubmed
Bhadula,
Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line.
2001,
Pubmed
Briolant,
Plasmodium falciparum proteome changes in response to doxycycline treatment.
2010,
Pubmed
Brock,
A secreted factor represses cell proliferation in Dictyostelium.
2005,
Pubmed
Buckley,
The cellular response to heat stress in the goby Gillichthys mirabilis: a cDNA microarray and protein-level analysis.
2006,
Pubmed
Calderwood,
Heat shock proteins in cancer: chaperones of tumorigenesis.
2006,
Pubmed
Campbell,
Three muscular dystrophies: loss of cytoskeleton-extracellular matrix linkage.
1995,
Pubmed
Clerico,
How hsp70 molecular machines interact with their substrates to mediate diverse physiological functions.
2015,
Pubmed
Cordeiro,
Changes in liver proteome expression of Senegalese sole (Solea senegalensis) in response to repeated handling stress.
2012,
Pubmed
Daugaard,
The heat shock protein 70 family: Highly homologous proteins with overlapping and distinct functions.
2007,
Pubmed
Dickinson,
Glutathione in defense and signaling: lessons from a small thiol.
2002,
Pubmed
Dingledine,
The glutamate receptor ion channels.
1999,
Pubmed
Eaton,
Concise review of the glutathione S-transferases and their significance to toxicology.
1999,
Pubmed
Fields,
Proteomic responses of blue mussel (Mytilus) congeners to temperature acclimation.
2012,
Pubmed
Gunst,
Actin cytoskeletal dynamics in smooth muscle: a new paradigm for the regulation of smooth muscle contraction.
2008,
Pubmed
Hasanuzzaman,
Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants.
2013,
Pubmed
Heunis,
Proteomic profiling of the acid stress response in Lactobacillus plantarum 423.
2014,
Pubmed
Holcik,
Translational control in stress and apoptosis.
2005,
Pubmed
Höhfeld,
Role of the chaperonin cofactor Hsp10 in protein folding and sorting in yeast mitochondria.
1994,
Pubmed
Ikonen,
Prohibitin, an antiproliferative protein, is localized to mitochondria.
1995,
Pubmed
Ji,
iTRAQ-based quantitative proteomic analyses on the gender-specific responses in mussel Mytilus galloprovincialis to tetrabromobisphenol A.
2014,
Pubmed
Jo,
Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase.
2001,
Pubmed
Karp,
Addressing accuracy and precision issues in iTRAQ quantitation.
2010,
Pubmed
Liu,
Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery.
2014,
Pubmed
Maher,
The effects of stress and aging on glutathione metabolism.
2005,
Pubmed
Marushige,
Alterations in focal adhesion and cytoskeletal proteins during apoptosis.
1998,
Pubmed
Miller,
An overview of the potassium channel family.
2000,
Pubmed
Mogk,
Cooperation of Hsp70 and Hsp100 chaperone machines in protein disaggregation.
2015,
Pubmed
Pajares,
Betaine homocysteine S-methyltransferase: just a regulator of homocysteine metabolism?
2006,
Pubmed
Parsell,
The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.
1993,
Pubmed
Queitsch,
Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis.
2000,
Pubmed
Richter,
Hsp90: chaperoning signal transduction.
2001,
Pubmed
Rudra,
What better measure than ribosome synthesis?
2004,
Pubmed
Skiba,
Human hepatic methionine biosynthesis. Purification and characterization of betaine:homocysteine S-methyltransferase.
1982,
Pubmed
Soffer,
Angiotensin-converting enzyme and the regulation of vasoactive peptides.
1976,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Sun,
Understanding the regulation of estivation in a freshwater snail through iTRAQ-based comparative proteomics.
2013,
Pubmed
Susin,
Molecular characterization of mitochondrial apoptosis-inducing factor.
1999,
Pubmed
Suzuki,
Chaperone properties of mammalian mitochondrial translation elongation factor Tu.
2007,
Pubmed
Teng,
Deletion of betaine-homocysteine S-methyltransferase in mice perturbs choline and 1-carbon metabolism, resulting in fatty liver and hepatocellular carcinomas.
2011,
Pubmed
Thorne,
Transcription profiling of acute temperature stress in the Antarctic plunderfish Harpagifer antarcticus.
2010,
Pubmed
Thorpe,
Structure and mechanism of action of the acyl-CoA dehydrogenases.
1995,
Pubmed
Tomanek,
Proteomics to study adaptations in marine organisms to environmental stress.
2014,
Pubmed
Vizcaíno,
ProteomeXchange provides globally coordinated proteomics data submission and dissemination.
2014,
Pubmed
Wang,
Parental Effect of Long Acclimatization on Thermal Tolerance of Juvenile Sea Cucumber Apostichopus japonicus.
2015,
Pubmed
,
Echinobase
Warner,
The economics of ribosome biosynthesis in yeast.
1999,
Pubmed
Wei,
Comparative proteomic analysis of Bactrocera dorsalis (Hendel) in response to thermal stress.
2015,
Pubmed
Weidenhaupt,
Bradyrhizobium japonicum possesses two discrete sets of electron transfer flavoprotein genes: fixA, fixB and etfS, etfL.
1996,
Pubmed
Wu,
Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF.
2006,
Pubmed
Xu,
Molecular cloning of heat shock protein 10 (Hsp10) and 60 (Hsp60) cDNAs and their expression analysis under thermal stress in the sea cucumber Apostichopus japonicus.
2014,
Pubmed
,
Echinobase
Xu,
Histological, ultrastructural and heat shock protein 70 (HSP70) responses to heat stress in the sea cucumber Apostichopus japonicus.
2015,
Pubmed
,
Echinobase
Young,
Hsp90: a specialized but essential protein-folding tool.
2001,
Pubmed
Zhang,
Identification of differential expressed proteins and characterization their mRNA expression in thermally stressed Apostichopus japonicus.
2013,
Pubmed
,
Echinobase
Zhao,
The molecular characterization and expression of heat shock protein 90 (Hsp90) and 26 (Hsp26) cDNAs in sea cucumber (Apostichopus japonicus).
2011,
Pubmed
,
Echinobase
Zieske,
A perspective on the use of iTRAQ reagent technology for protein complex and profiling studies.
2006,
Pubmed
