Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
Procedures for isolating the contents of the cortical granules from the ova of the sea urchin, Strongylocentrotus purpuratus, are reported. Dithiothreitol is used to remove the vitelline coat; the "demembranated" eggs are then subsequently activated with butyric acid. By means of these procedures, the hyaline protein and crystalline or paracrystalline material have been isolated from the cortical granules. The crystalline material consists of sheets of cylinders or tubules 150-200 A in diameter. This material is believed to be a major structural element of the fertilization membrane which, in the absence of the vitelline coat, does not form.
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968, Pubmed,
Echinobase
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968,
Pubmed
,
Echinobase
ENDO,
Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I. Clypeaster japonicus.
1961,
Pubmed
,
Echinobase
Epel,
Beta-1,3-glucanase of sea urchin eggs: release from particles at fertilization.
1969,
Pubmed
,
Echinobase
Humphreys,
The fine structure of cortical granules in eggs and gastrulae of Mytilus edulis.
1967,
Pubmed
IMMERS,
Comparative study of the localization of incorporated 14C-labeled amino acids and 35SO4 in the sea urchin ovary, egg and embryo.
1961,
Pubmed
,
Echinobase
ISHIHARA,
RELEASE OF ACID POLYSACCHARIDES FOLLOWING FERTILIZATION OF SEA URCHIN EGGS. A CHEMICAL STUDY OF CHANGES IN THE CELL SURFACE.
1964,
Pubmed
,
Echinobase
Inoue,
Fertilization membranes structure analysis with the surface replica method.
1967,
Pubmed
Ishihara,
Chemical analysis of glycoproteins in the egg surface of the sea urchin, Arbacia punctulata.
1968,
Pubmed
,
Echinobase
Ishihara,
An analysis of acid polysaccharides produced at fertilization of sea urchin.
1968,
Pubmed
,
Echinobase
KANE,
The isolation and preliminary characterization of a major soluble protein of the sea urchin egg.
1959,
Pubmed
,
Echinobase
Kane,
A comparative study of the isolation of the cortex and the role of the calcium-insoluble protein in several species of sea urchin egg.
1969,
Pubmed
,
Echinobase
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
MEHL,
Acid and base production at fertilization in the sea urchin.
1961,
Pubmed
,
Echinobase
SAKAI,
Studies on sulfhydryl groups during cell division of sea urchin egg. II. Mass isolation of the egg cortex and change in its--SH groups during cell division.
1960,
Pubmed
,
Echinobase
WOLPERT,
An electron microscope study of fertilisation of the sea urchin egg Psammechinus miliaris.
1961,
Pubmed
,
Echinobase
