Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Protein Sci
2017 Aug 01;268:1574-1583. doi: 10.1002/pro.3185.
Show Gene links
Show Anatomy links
Carbohydrate recognition by the rhamnose-binding lectin SUL-I with a novel three-domain structure isolated from the venom of globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus.
Hatakeyama T
,
Ichise A
,
Unno H
,
Goda S
,
Oda T
,
Tateno H
,
Hirabayashi J
,
Sakai H
,
Nakagawa H
.
???displayArticle.abstract???
The globiferous pedicellariae of the venomous sea urchin Toxopneustes pileolus contains several biologically active proteins. We have cloned the cDNA of one of the toxin components, SUL-I, which is a rhamnose-binding lectin (RBL) that acts as a mitogen through binding to carbohydrate chains on target cells. Recombinant SUL-I (rSUL-I) was produced in Escherichia coli cells, and its carbohydrate-binding specificity was examined with the glycoconjugate microarray analysis, which suggested that potential target carbohydrate structures are galactose-terminated N-glycans. rSUL-I exhibited mitogenic activity for murine splenocyte cells and toxicity against Vero cells. The three-dimensional structure of the rSUL-I/l-rhamnose complex was determined by X-ray crystallographic analysis at a 1.8 Å resolution. The overall structure of rSUL-I is composed of three distinctive domains with a folding structure similar to those of CSL3, a RBL from chum salmon (Oncorhynchus keta) eggs. The bound l-rhamnose molecules are mainly recognized by rSUL-I through hydrogen bonds between its 2-, 3-, and 4-hydroxy groups and Asp, Asn, and Glu residues in the binding sites, while Tyr and Ser residues participate in the recognition mechanism. It was also inferred that SUL-I may form a dimer in solution based on the molecular size estimated via dynamic light scattering as well as possible contact regions in its crystal structure.
Collaborative Computational Project, Number 4,
The CCP4 suite: programs for protein crystallography.
1994, Pubmed
Collaborative Computational Project, Number 4,
The CCP4 suite: programs for protein crystallography.
1994,
Pubmed
Emsley,
Features and development of Coot.
2010,
Pubmed
Evans,
Scaling and assessment of data quality.
2006,
Pubmed
Hatakeyama,
An assay for carbohydrate-binding activity of lectins using polyamidoamine dendrimer conjugated with carbohydrates.
2012,
Pubmed
,
Echinobase
Hatakeyama,
cDNA cloning and characterization of a rhamnose-binding lectin SUL-I from the toxopneustid sea urchin Toxopneustes pileolus venom.
2015,
Pubmed
,
Echinobase
Hirabayashi,
Lectin-based structural glycomics: glycoproteomics and glycan profiling.
2004,
Pubmed
Hu,
Tailoring GalNAcα1-3Galβ-specific lectins from a multi-specific fungal galectin: dramatic change of carbohydrate specificity by a single amino-acid substitution.
2013,
Pubmed
Krissinel,
Enhanced fold recognition using efficient short fragment clustering.
2012,
Pubmed
Krissinel,
Inference of macromolecular assemblies from crystalline state.
2007,
Pubmed
Leslie,
The integration of macromolecular diffraction data.
2006,
Pubmed
McCoy,
Likelihood-enhanced fast translation functions.
2005,
Pubmed
Murshudov,
Refinement of macromolecular structures by the maximum-likelihood method.
1997,
Pubmed
Nakagawa,
Biochemical and physiological properties of pedicellarial lectins from the toxopneustid sea urchins.
1999,
Pubmed
,
Echinobase
Nakamura,
Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin.
2005,
Pubmed
Oda,
Temperature- and pH-dependent cytotoxic effect of the hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata on various cell lines.
1997,
Pubmed
,
Echinobase
Ogawa,
Diversified carbohydrate-binding lectins from marine resources.
2011,
Pubmed
Shirai,
Structure of rhamnose-binding lectin CSL3: unique pseudo-tetrameric architecture of a pattern recognition protein.
2009,
Pubmed
Takei,
A sea urchin lectin, SUL-1, from the Toxopneustid sea urchin induces DC maturation from human monocyte and drives Th1 polarization in vitro.
2006,
Pubmed
,
Echinobase
Tateno,
Evaluation of glycan-binding specificity by glycoconjugate microarray with an evanescent-field fluorescence detection system.
2014,
Pubmed
Tateno,
Glycoconjugate microarray based on an evanescent-field fluorescence-assisted detection principle for investigation of glycan-binding proteins.
2008,
Pubmed
Tateno,
SUEL-related lectins, a lectin family widely distributed throughout organisms.
2010,
Pubmed
,
Echinobase
Watanabe,
The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3.
2009,
Pubmed
