Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Am Soc Mass Spectrom
2018 May 01;295:923-934. doi: 10.1007/s13361-018-1898-x.
Show Gene links
Show Anatomy links
Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry.
Monroe EB
,
Annangudi SP
,
Wadhams AA
,
Richmond TA
,
Yang N
,
Southey BR
,
Romanova EV
,
Schoofs L
,
Baggerman G
,
Sweedler JV
.
???displayArticle.abstract???
Neuropeptides are essential cell-to-cell signaling messengers and serve important regulatory roles in animals. Although remarkable progress has been made in peptide identification across the Metazoa, for some phyla such as Echinodermata, limited neuropeptides are known and even fewer have been verified on the protein level. We employed peptidomic approaches using bioinformatics and mass spectrometry (MS) to experimentally confirm 23 prohormones and to characterize a new prohormone in nervous systemtissue from Strongylocentrotus purpuratus, the purple sea urchin. Ninety-three distinct peptides from known and novel prohormones were detected with MS from extracts of the radial nerves, many of which are reported or experimentally confirmed here for the first time, representing a large-scale study of neuropeptides from the phylum Echinodermata. Many of the identified peptides and their precursor proteins have low homology to known prohormones from other species/phyla and are unique to the sea urchin. By pairing bioinformatics with MS, the capacity to characterize novel peptides and annotate prohormone genes is enhanced. Graphical Abstract.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Amare,
Neuropeptide precursors in Tribolium castaneum.
2007,
Pubmed
Amare,
Bridging neuropeptidomics and genomics with bioinformatics: Prediction of mammalian neuropeptide prohormone processing.
2006,
Pubmed
Bendtsen,
Improved prediction of signal peptides: SignalP 3.0.
2004,
Pubmed
Boonen,
Peptidomics: the integrated approach of MS, hyphenated techniques and bioinformatics for neuropeptide analysis.
2008,
Pubmed
Burke,
A genomic view of the sea urchin nervous system.
2006,
Pubmed
,
Echinobase
Collins,
Genome-wide analyses reveal a role for peptide hormones in planarian germline development.
2010,
Pubmed
Elphick,
NG peptides: a novel family of neurophysin-associated neuropeptides.
2010,
Pubmed
,
Echinobase
Elphick,
Molecular characterisation of SALMFamide neuropeptides in sea urchins.
2005,
Pubmed
,
Echinobase
Elphick,
The evolution and diversity of SALMFamide neuropeptides.
2013,
Pubmed
,
Echinobase
Elphick,
From gonadotropin-inhibitory hormone to SIFamides: are echinoderm SALMFamides the "missing link" in a bilaterian family of neuropeptides that regulate reproductive processes?
2013,
Pubmed
,
Echinobase
Elphick,
The SALMFamides: a new family of neuropeptides isolated from an echinoderm.
1991,
Pubmed
,
Echinobase
Elphick,
Neural control of muscle relaxation in echinoderms.
2001,
Pubmed
,
Echinobase
Hall,
Involvement of pedal peptide in locomotion in Aplysia: modulation of foot muscle contractions.
1990,
Pubmed
Hatcher,
Mass spectrometry-based discovery of circadian peptides.
2008,
Pubmed
Hewes,
Neuropeptides and neuropeptide receptors in the Drosophila melanogaster genome.
2001,
Pubmed
Hu,
Identification of prohormones and pituitary neuropeptides in the African cichlid, Astatotilapia burtoni.
2016,
Pubmed
Huerta-Cepas,
ETE 3: Reconstruction, Analysis, and Visualization of Phylogenomic Data.
2016,
Pubmed
Hummon,
From precursor to final peptides: a statistical sequence-based approach to predicting prohormone processing.
2003,
Pubmed
Hummon,
From the genome to the proteome: uncovering peptides in the Apis brain.
2006,
Pubmed
Huttner,
Regulated secretion. Helper proteins for neuroendocrine secretion.
1995,
Pubmed
Jiménez,
Peptidomics of a single identified neuron reveals diversity of multiple neuropeptides with convergent actions on cellular excitability.
2006,
Pubmed
Jing,
From hunger to satiety: reconfiguration of a feeding network by Aplysia neuropeptide Y.
2007,
Pubmed
Jékely,
Global view of the evolution and diversity of metazoan neuropeptide signaling.
2013,
Pubmed
Lee,
Endogenous peptide discovery of the rat circadian clock: a focused study of the suprachiasmatic nucleus by ultrahigh performance tandem mass spectrometry.
2010,
Pubmed
Lin,
Pedal peptide/orcokinin-type neuropeptide signaling in a deuterostome: The anatomy and pharmacology of starfish myorelaxant peptide in Asterias rubens.
2017,
Pubmed
,
Echinobase
Liu,
The construction of a bioactive peptide database in Metazoa.
2008,
Pubmed
Lloyd,
Sequence of pedal peptide: a novel neuropeptide from the central nervous system of Aplysia.
1989,
Pubmed
Ma,
Expanding the Crustacean neuropeptidome using a multifaceted mass spectrometric approach.
2009,
Pubmed
Mbikay,
Neuroendocrine secretory protein 7B2: structure, expression and functions.
2001,
Pubmed
Menschaert,
Peptidomics coming of age: a review of contributions from a bioinformatics angle.
2010,
Pubmed
Menschaert,
A hybrid, de novo based, genome-wide database search approach applied to the sea urchin neuropeptidome.
2010,
Pubmed
,
Echinobase
Merkler,
C-terminal amidated peptides: production by the in vitro enzymatic amidation of glycine-extended peptides and the importance of the amide to bioactivity.
1994,
Pubmed
Motokawa,
Coordination between catch connective tissue and muscles through nerves in the spine joint of the sea urchin Diadema setosum.
2015,
Pubmed
,
Echinobase
NCBI Resource Coordinators,
Database Resources of the National Center for Biotechnology Information.
2017,
Pubmed
Orekhova,
Multiple presynaptic and postsynaptic sites of inhibitory modulation by myomodulin at ARC neuromuscular junctions of Aplysia.
2003,
Pubmed
Perkins,
Probability-based protein identification by searching sequence databases using mass spectrometry data.
1999,
Pubmed
Prigge,
New insights into copper monooxygenases and peptide amidation: structure, mechanism and function.
2000,
Pubmed
Riehle,
Neuropeptides and peptide hormones in Anopheles gambiae.
2002,
Pubmed
Romanova,
Autonomic control network active in Aplysia during locomotion includes neurons that express splice variants of R15-neuropeptides.
2007,
Pubmed
Romanova,
Peptidomics for the discovery and characterization of neuropeptides and hormones.
2015,
Pubmed
Romanova,
Differential peptidomics assessment of strain and age differences in mice in response to acute cocaine administration.
2015,
Pubmed
Rowe,
The neuropeptide transcriptome of a model echinoderm, the sea urchin Strongylocentrotus purpuratus.
2012,
Pubmed
,
Echinobase
Rowe,
Discovery of a second SALMFamide gene in the sea urchin Strongylocentrotus purpuratus reveals that L-type and F-type SALMFamide neuropeptides coexist in an echinoderm species.
2010,
Pubmed
,
Echinobase
Satoh,
Chordate evolution and the three-phylum system.
2014,
Pubmed
,
Echinobase
Schalch,
Abnormalities in the release of TSH in response to thyrotropin-releasing hormone (TRH) in patients with disorders of the pituitary, hypothalamus and basal ganglia.
1972,
Pubmed
Schulz-Knappe,
The peptidomics concept.
2005,
Pubmed
Semmens,
Transcriptomic identification of starfish neuropeptide precursors yields new insights into neuropeptide evolution.
2016,
Pubmed
,
Echinobase
Semmens,
The evolution of neuropeptide signalling: insights from echinoderms.
2017,
Pubmed
,
Echinobase
Sigafoos,
Novel peptides from adrenomedullary chromaffin vesicles.
1993,
Pubmed
Smith,
The neuropeptidome of the Crown-of-Thorns Starfish, Acanthaster planci.
2017,
Pubmed
,
Echinobase
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Southey,
NeuroPred: a tool to predict cleavage sites in neuropeptide precursors and provide the masses of the resulting peptides.
2006,
Pubmed
Southey,
Characterization of the prohormone complement in cattle using genomic libraries and cleavage prediction approaches.
2009,
Pubmed
Sweedler,
Identification and characterization of the feeding circuit-activating peptides, a novel neuropeptide family of aplysia.
2002,
Pubmed
Tashjian,
Thyrotropin releasing hormone: direct evidence for stimulation of prolactin production by pituitary cells in culture.
1971,
Pubmed
The UniProt Consortium,
UniProt: the universal protein knowledgebase.
2017,
Pubmed
Ullrich-Lüter,
Unique system of photoreceptors in sea urchin tube feet.
2011,
Pubmed
,
Echinobase
Vanden Broeck,
Neuropeptides and their precursors in the fruitfly, Drosophila melanogaster.
2001,
Pubmed
Westphal,
The neuroendocrine protein 7B2 is required for peptide hormone processing in vivo and provides a novel mechanism for pituitary Cushing's disease.
1999,
Pubmed
Xie,
The zebra finch neuropeptidome: prediction, detection and expression.
2010,
Pubmed
Ye,
Defining the Neuropeptidome of the Spiny Lobster Panulirus interruptus Brain Using a Multidimensional Mass Spectrometry-Based Platform.
2015,
Pubmed
Yin,
Neuropeptidomics: mass spectrometry-based qualitative and quantitative analysis.
2011,
Pubmed
Yun,
Identification of novel SALMFamide neuropeptides in the starfish Marthasterias glacialis.
2007,
Pubmed
,
Echinobase
Zhang,
PEAKS DB: de novo sequencing assisted database search for sensitive and accurate peptide identification.
2012,
Pubmed
