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PLoS One
2013 Jan 01;81:e53087. doi: 10.1371/journal.pone.0053087.
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Frondoside a suppressive effects on lung cancer survival, tumor growth, angiogenesis, invasion, and metastasis.
Attoub S
,
Arafat K
,
Gélaude A
,
Al Sultan MA
,
Bracke M
,
Collin P
,
Takahashi T
,
Adrian TE
,
De Wever O
.
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A major challenge for oncologists and pharmacologists is to develop less toxic drugs that will improve the survival of lung cancer patients. Frondoside A is a triterpenoid glycoside isolated from the sea cucumber, Cucumaria frondosa and was shown to be a highly safe compound. We investigated the impact of Frondoside A on survival, migration and invasion in vitro, and on tumor growth, metastasis and angiogenesis in vivo alone and in combination with cisplatin. Frondoside A caused concentration-dependent reduction in viability of LNM35, A549, NCI-H460-Luc2, MDA-MB-435, MCF-7, and HepG2 over 24 hours through a caspase 3/7-dependent cell death pathway. The IC50 concentrations (producing half-maximal inhibition) at 24 h were between 1.7 and 2.5 µM of Frondoside A. In addition, Frondoside A induced a time- and concentration-dependent inhibition of cell migration, invasion and angiogenesis in vitro. Frondoside A (0.01 and 1 mg/kg/day i.p. for 25 days) significantly decreased the growth, the angiogenesis and lymph node metastasis of LNM35 tumor xenografts in athymic mice, without obvious toxic side-effects. Frondoside A (0.1-0.5 µM) also significantly prevented basal and bFGF induced angiogenesis in the CAM angiogenesis assay. Moreover, Frondoside A enhanced the inhibition of lung tumor growth induced by the chemotherapeutic agent cisplatin. These findings identify Frondoside A as a promising novel therapeutic agent for lung cancer.
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23308143
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Figure 4. The anti-angiogenic activity of Frondoside A in the xenograft tumor:Immuno-histochemical staining of lung xenograft tumors for CD31 (microvessel density).
Figure 5. The anti-angiogenic activity of Frondoside A in the Chorioallantoic membrane (CAM) assay in vivo:CAM was treated with control (serum-free medium), 100 nM Frondoside A, 500 nM Frondoside A, 2 µg/mL bFGF, 2 µg/mL bFGF+500 nM Frondoside A and the vascularization of test discs was photographed.
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,
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,
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,
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Jin,
Differential effects of triterpene glycosides, frondoside A and cucumarioside A2-2 isolated from sea cucumbers on caspase activation and apoptosis of human leukemia cells.
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,
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Korpanty,
Update on anti-angiogenic therapy in non-small cell lung cancer: Are we making progress?
2011,
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Kozaki,
Establishment and characterization of a human lung cancer cell line NCI-H460-LNM35 with consistent lymphogenous metastasis via both subcutaneous and orthotopic propagation.
2000,
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Li,
Review of the apoptosis pathways in pancreatic cancer and the anti-apoptotic effects of the novel sea cucumber compound, Frondoside A.
2008,
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,
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Ma,
Frondoside A inhibits breast cancer metastasis and antagonizes prostaglandin E receptors EP4 and EP2.
2012,
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,
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Mann,
Natural products in cancer chemotherapy: past, present and future.
2002,
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Molinski,
Drug development from marine natural products.
2009,
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Roginsky,
Anti-pancreatic cancer effects of a polar extract from the edible sea cucumber, Cucumaria frondosa.
2010,
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,
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Spira,
Multidisciplinary management of lung cancer.
2004,
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