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ECB-ART-32564
Biophys J 1988 Jun 01;536:1015-9. doi: 10.1016/S0006-3495(88)83181-3.
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Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope.

Kinosita K , Ashikawa I , Saita N , Yoshimura H , Itoh H , Nagayama K , Ikegami A .


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Controlled permeability can be conferred to cell membranes by exposing cells to a microsecond electric pulse of sufficient intensity (electroporation). By constructing a fluorescence microimaging system with a submicrosecond time resolution we have been able to resolve temporally and spatially the events in a single cell under a microsecond electric pulse. An enormous membrane conductance, corresponding to a loss of 0.01-0.1% of the membrane area, was observed in those membrane regions where the transmembrane potential induced by the electric pulse exceeded a critical value. The conductance decreased to a low level in a submillisecond after the pulse, leaving a moderately electroporated cell.

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Genes referenced: LOC115919910

References [+] :
Ehrenberg, Membrane potential induced by external electric field pulses can be followed with a potentiometric dye. 1987, Pubmed