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Mol Cell Biol
1994 Feb 01;142:1402-9. doi: 10.1128/mcb.14.2.1402-1409.1994.
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Purification of an oligo(dG).oligo(dC)-binding sea urchin nuclear protein, suGF1: a family of G-string factors involved in gene regulation during development.
Hapgood J
,
Patterton D
.
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Contiguous deoxyguanosine residues (G strings) have been implicated in regulation of gene expression in several organisms via the binding of G-string factors. Regulation of expression of the chicken adult beta-globin gene may involve the interplay between binding of an erythrocyte-specific G-string factor, BGP1, and the stability of a positioned nucleosome (C. D. Lewis, S. P. Clark, G. Felsenfeld, and H. Gould, Genes Dev. 2:863-873, 1988). We have purified a 59.5-kDa nuclear protein (suGF1) from sea urchin embryos by DNA affinity chromatography. suGF1 has high binding affinity and specificity for oligo(dG).oligo(dC). The identity of the purified protein was confirmed by renaturation of sequence-specific DNA-binding activity from a sodium dodecyl sulfate-polyacrylamide gel slice and by Southwestern (DNA-protein) blotting. suGF1 binds in vitro to a G11 string present in the H1-H4 intergenic region of a sea urchin early histone gene battery. This suGF1 DNA recognition site occurs within a homopurine-homopyrimidine stretch previously shown to be incorporated into a positioned nucleosome core in vitro. DNase I footprinting shows that suGF1 protects the same base pairs on the promoter of the chicken beta A-globin gene as does BGP1. We show that a G-string cis-regulatory element of a sea urchin cell lineage-specific gene LpS1 (M. Xiang, S.-Y. Lu, M. Musso, G. Karsenty, and W. H. Klein, Development 113:1345-1355, 1991) also represents a high-affinity recognition site for suGF1. suGF1 may be a member of a family of G-string factors involved in the regulation of expression of unrelated genes during development of a number of different organisms.
Barberis,
Mutually exclusive interaction of the CCAAT-binding factor and of a displacement protein with overlapping sequences of a histone gene promoter.
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Barberis,
Mutually exclusive interaction of the CCAAT-binding factor and of a displacement protein with overlapping sequences of a histone gene promoter.
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,
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Barton,
The erythroid protein cGATA-1 functions with a stage-specific factor to activate transcription of chromatin-assembled beta-globin genes.
1993,
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Calzone,
Gene regulatory factors of the sea urchin embryo. I. Purification by affinity chromatography and cloning of P3A2, a novel DNA-binding protein.
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,
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Clark,
Properties of BGP1, a poly(dG)-binding protein from chicken erythrocytes.
1990,
Pubmed
Dailey,
Purification of the human histone H4 gene-specific transcription factors H4TF-1 and H4TF-2.
1988,
Pubmed
Davis,
Ribonucleoprotein and protein factors bind to an H-DNA-forming c-myc DNA element: possible regulators of the c-myc gene.
1989,
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Emerson,
Interaction of specific nuclear factors with the nuclease-hypersensitive region of the chicken adult beta-globin gene: nature of the binding domain.
1985,
Pubmed
Emerson,
Erythroid-specific activation and derepression of the chick beta-globin promoter in vitro.
1989,
Pubmed
Hall,
Regulation of c-myc transcription in vitro: dependence on the guanine-rich promoter element ME1a1.
1990,
Pubmed
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Hentschel,
Homocopolymer sequences in the spacer of a sea urchin histone gene repeat are sensitive to S1 nuclease.
1982,
Pubmed
,
Echinobase
Hoffman,
An S1 nuclease-sensitive homopurine/homopyrimidine domain in the c-Ki-ras promoter interacts with a nuclear factor.
1990,
Pubmed
Jackson,
A method for mapping intranuclear protein-DNA interactions and its application to a nuclease hypersensitive site.
1985,
Pubmed
Johnson,
Modulation of epidermal growth factor receptor proto-oncogene transcription by a promoter site sensitive to S1 nuclease.
1988,
Pubmed
Kadonaga,
Purification of sequence-specific binding proteins by DNA affinity chromatography.
1991,
Pubmed
Kadonaga,
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
1987,
Pubmed
Karsenty,
Conservation of binding sites for regulatory factors in the coordinately expressed alpha 1 (I) and alpha 2 (I) collagen promoters.
1991,
Pubmed
Kefalas,
Precise nucleosome positioning in the promoter of the chicken beta A globin gene.
1988,
Pubmed
Kohwi,
Intramolecular dG.dG.dC triplex detected in Escherichia coli cells.
1992,
Pubmed
Kohwi,
Altered gene expression correlates with DNA structure.
1991,
Pubmed
Kohwi-Shigematsu,
Poly(dG)-poly(dC) sequences, under torsional stress, induce an altered DNA conformation upon neighboring DNA sequences.
1985,
Pubmed
Lewis,
An erythrocyte-specific protein that binds to the poly(dG) region of the chicken beta-globin gene promoter.
1988,
Pubmed
Lobanenkov,
A novel sequence-specific DNA binding protein which interacts with three regularly spaced direct repeats of the CCCTC-motif in the 5'-flanking sequence of the chicken c-myc gene.
1990,
Pubmed
McKeon,
A sequence conserved in both the chicken and mouse alpha 2(I) collagen promoter contains sites sensitive to S1 nuclease.
1984,
Pubmed
Patterton,
Negative supercoiling and nucleosome cores. I. The effect of negative supercoiling on the efficiency of nucleosome core formation in vitro.
1993,
Pubmed
Patterton,
Negative supercoiling and nucleosome cores. II. The effect of negative supercoiling on the positioning of nucleosome cores in vitro.
1993,
Pubmed
,
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Patterton,
suGF1 binds in the major groove of its oligo(dG).oligo(dC) recognition sequence and is excluded by a positioned nucleosome core.
1994,
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,
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Postel,
A nuclease-hypersensitive element of the human c-myc promoter interacts with a transcription initiation factor.
1989,
Pubmed
Schon,
Conformation of promoter DNA: fine mapping of S1-hypersensitive sites.
1983,
Pubmed
Simpson,
Nucleosome positioning: occurrence, mechanisms, and functional consequences.
1991,
Pubmed
Tewari,
Characterization of the promoter region and 3' end of the human insulin receptor gene.
1989,
Pubmed
Wells,
The chemistry and biology of unusual DNA structures adopted by oligopurine.oligopyrimidine sequences.
1988,
Pubmed
Wu,
Transient alterations of the chromatin structure of sea urchin early histone genes during embryogenesis.
1985,
Pubmed
,
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Xiang,
A G-string positive cis-regulatory element in the LpS1 promoter binds two distinct nuclear factors distributed non-uniformly in Lytechinus pictus embryos.
1991,
Pubmed
,
Echinobase
von Holt,
Isolation and characterization of histones.
1989,
Pubmed
,
Echinobase
