Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1987 Aug 01;1052:927-36. doi: 10.1083/jcb.105.2.927.
Show Gene links
Show Anatomy links
Low ionic strength solubility of myosin in sea urchin egg extracts is mediated by a myosin-binding protein.
Yabkowitz R
,
Burgess DR
.
???displayArticle.abstract???
We identify a novel myosin-binding protein, designated 53K, which appears to mediate the low ionic strength solubility of myosin in extracts of unfertilized sea urchin eggs. The protein possesses a subunit molecular mass on SDS-PAGE of 53 kD, an S value of 7, may be organized into disulfide-linked oligomers, and is associated with myosin in egg extracts. Both myosin and 53K co-precipitate from extract upon the addition of nucleoside triphosphates and co-sediment with an S value of 24 by sedimentation velocity centrifugation. Myosin in extracts not associated with 53K has an S value of 10. Further, myosin can be immunoprecipitated from extract with antibody to 53K and the 53K in extracts binds to a myosin affinity column. When extract is depleted of 53K, a majority of the myosin precipitates out of extract in a nucleotide-independent manner. Whereas purified myosin precipitates in the absence of nucleotide when recombined with dialysis buffer or myosin-depleted extract, reconstituting 53K and myosin before addition to buffer or myosin-depleted extract partially restores the low ionic strength solubility demonstrated by myosin in fresh egg extracts. The 53-kD protein may represent a new class of authentic myosin-binding proteins that may regulate the supramolecular organization of myosin in nonmuscle cells.
Berlot,
Chemoattractant-elicited increases in myosin phosphorylation in Dictyostelium.
1985, Pubmed
Berlot,
Chemoattractant-elicited increases in myosin phosphorylation in Dictyostelium.
1985,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Broschat,
Phosphorylation controls brush border motility by regulating myosin structure and association with the cytoskeleton.
1983,
Pubmed
Bähler,
Novel thick filament protein of chicken pectoralis muscle: the 86 kd protein. II. Distribution and localization.
1985,
Pubmed
Clarke,
Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination.
1977,
Pubmed
,
Echinobase
Collins,
Comparison of the actin binding and filament formation properties of phosphorylated and dephosphorylated Acanthamoeba myosin II.
1982,
Pubmed
Collins,
Actin activation of Ca2+-sensitive Mg2+-ATPase activity of Acanthamoeba myosin II is enhanced by dephosphorylation of its heavy chains.
1980,
Pubmed
Condeelis,
The contractile basis of amoeboid movement. V. The control of gelation, solation, and contraction in extracts from Dictyostelium discoideum.
1977,
Pubmed
Craig,
Light-chain phosphorylation controls the conformation of vertebrate non-muscle and smooth muscle myosin molecules.
1983,
Pubmed
Eppenberger,
The Mr 165,000 M-protein myomesin: a specific protein of cross-striated muscle cells.
1981,
Pubmed
Harrington,
Myosin.
1984,
Pubmed
Horowits,
A physiological role for titin and nebulin in skeletal muscle.
1986,
Pubmed
Ishimoda-Takagi,
Localization of tropomyosin in sea urchin eggs.
1979,
Pubmed
,
Echinobase
Jeacocke,
Phosphorylation of a myofibrillar protein of Mr 150 000 in perfused rat heart, and the tentative indentification of this as C-protein.
1980,
Pubmed
Kane,
Preparation and purification of polymerized actin from sea urchin egg extracts.
1975,
Pubmed
,
Echinobase
Kane,
Actin polymerization and interaction with other proteins in temperature-induced gelation of sea urchin egg extracts.
1976,
Pubmed
,
Echinobase
Kane,
Induction of either contractile or structural actin-based gels in sea urchin egg cytoplasmic extract.
1980,
Pubmed
,
Echinobase
Kane,
Interconversion of structural and contractile actin gels by insertion of myosin during assembly.
1983,
Pubmed
,
Echinobase
Kuczmarski,
Regulation of myosin self-assembly: phosphorylation of Dictyostelium heavy chain inhibits formation of thick filaments.
1980,
Pubmed
Kuźnicki,
Phosphorylation of myosin in non-muscle and smooth muscle cells. Possible rules and evolutionary trends.
1986,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Mabuchi,
Alpha-actinin from sea urchin eggs: biochemical properties, interaction with actin, and distribution in the cell during fertilization and cleavage.
1985,
Pubmed
,
Echinobase
Maruta,
Myosin heavy chain kinase inactivated by Ca2+/calmodulin from aggregating cells of Dictyostelium discoideum.
1983,
Pubmed
Maruyama,
Connectin filaments in stretched skinned fibers of frog skeletal muscle.
1984,
Pubmed
Matsudaira,
SDS microslab linear gradient polyacrylamide gel electrophoresis.
1978,
Pubmed
Moos,
Fluorescence microscope study of the binding of added C protein to skeletal muscle myofibrils.
1981,
Pubmed
Moos,
Effect of C-protein on actomyosin ATPase.
1980,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Ogihara,
Requirement of phosphorylation of Physarum myosin heavy chain for thick filament formation, actin activation of Mg2+-ATPase activity, and Ca2+-inhibitory superprecipitation.
1983,
Pubmed
Pollard,
The role of actin in the temperature-dependent gelation and contraction of extracts of Acanthamoeba.
1976,
Pubmed
Pollard,
Actin and actin-binding proteins. A critical evaluation of mechanisms and functions.
1986,
Pubmed
Reinach,
Isoforms of C-protein in adult chicken skeletal muscle: detection with monoclonal antibodies.
1982,
Pubmed
Reisler,
Macromolecular assemblies of myosin.
1986,
Pubmed
Shen,
Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg.
1978,
Pubmed
,
Echinobase
Stossel,
Interactions of actin, myosin, and a new actin-binding protein of rabbit pulmonary macrophages. II. Role in cytoplasmic movement and phagocytosis.
1976,
Pubmed
Trotter,
The heavy chain of macrophage myosin is phosphorylated at the tip of the tail.
1985,
Pubmed
Trybus,
Mechanism of smooth muscle myosin phosphorylation.
1985,
Pubmed
Trybus,
Conformational states of smooth muscle myosin. Effects of light chain phosphorylation and ionic strength.
1984,
Pubmed
Wang,
Sarcomere-associated cytoskeletal lattices in striated muscle. Review and hypothesis.
1985,
Pubmed
Yumura,
Reversible cyclic AMP-dependent change in distribution of myosin thick filaments in Dictyostelium.
1985,
Pubmed
