Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1977 Nov 01;752 Pt 1:410-21. doi: 10.1083/jcb.75.2.410.
Show Gene links
Show Anatomy links
Isolation and characterization of the vitelline layer of sea urchin eggs.
Glabe CG
,
Vacquier VD
.
???displayArticle.abstract???
The vitelline layers (VLs) of unfertilized sea urchin eggs were isolated by homogenization in a hypotonic medium containing Triton X-100 and EDTA. The surface topography of the VL is not changed by isolation. The thickness of the isolated VLs (300-400 A) is greater than that reported for VLs on intact eggs (100-200 A). Sperm adhere to the isolated VLs. When both internal and external VL surfaces are accessible to sperm, the sperm attach only to the external surface, suggesting that the external surface may carry sperm receptor proteins not present on the internal surface. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis shows that isolated VLs are composed of numerous proteins ranging from greater than 213,000 to 25,000 daltons. Lactoperoxidase-catalyzed 125I-iodination of unfertilized eggs labels two high molecular weight bands that stain faintly for carbohydrate. VLs are 90% protein and 3.5% carbohydrate. No predominance of a single amino acid or class of amino acids was found. Carbohydrate analysis yields fucose, mannose, galactose, glucose, xylose, glucosamine, galactosamine, and sialic acid. Controls for purity indicate that isolated VLs contain 2% protein of cytoplasmic origin and no more than 2.5% egg jelly.
Aketa,
Characterization of the sperm-binding protein from sea urchin egg surface.
1968, Pubmed,
Echinobase
Aketa,
Characterization of the sperm-binding protein from sea urchin egg surface.
1968,
Pubmed
,
Echinobase
Bryan,
The isolation of a major structural element of the sea urchin fertilization membrane.
1970,
Pubmed
,
Echinobase
Carroll,
Isolation and biological activity of the proteases released by sea urchin eggs following fertilization.
1975,
Pubmed
,
Echinobase
Epel,
Methods for removal of the vitelline membrane of sea urchin eggs. II. Controlled exposure to trypsin to eliminate post-fertilization clumping of embryos.
1970,
Pubmed
,
Echinobase
Epel,
Methods for revoval of the vitelline membrane of sea urchin eggs. I. Use of dithiothreitol (Cleland Reagent).
1970,
Pubmed
,
Echinobase
Fodor,
Isolation of a protease from sea urchin eggs before and after fertilization.
1975,
Pubmed
,
Echinobase
Glabe,
Species specific agglutination of eggs by bindin isolated from sea urchin sperm.
1977,
Pubmed
,
Echinobase
HUNTER,
Preparation of iodine-131 labelled human growth hormone of high specific activity.
1962,
Pubmed
Haino,
Studies on the egg-membrane lysin of Tegula pfeifferi: isolation and chemical analysis of the egg membrane.
1966,
Pubmed
Heller,
The vitelline envelope of eggs from the giant keyhole limpet Megathura crenulata. I. Chemical composition and structural studies.
1976,
Pubmed
Johnson,
Relationship between release of surface proteins and metabolic activation of sea urchin eggs at fertilization.
1975,
Pubmed
,
Echinobase
Kane,
Hyalin release during normal sea urchin development and its replacement after removal at fertilization.
1973,
Pubmed
,
Echinobase
Kim,
Analysis of neutral sugars by gas-liquid chromatography of alditol acetates: application to thyrotropic hormone and other glycoproteins.
1967,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Longo,
The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata.
1968,
Pubmed
,
Echinobase
SAKAI,
Studies on sulfhydryl groups during cell division of sea urchin egg. II. Mass isolation of the egg cortex and change in its--SH groups during cell division.
1960,
Pubmed
,
Echinobase
Schmell,
Identification of a sperm receptor on the surface of the eggs of the sea urchin Arbacia punctulata.
1977,
Pubmed
,
Echinobase
Shapiro,
Limited proteolysis of some egg surface components is an early event following fertilization of the sea urchin, Strongylocentrotus purpuratus.
1975,
Pubmed
,
Echinobase
Summers,
Species-specificity of acrosome reaction and primary gamete binding in echinoids.
1975,
Pubmed
,
Echinobase
Tegner,
Sea urchin sperm-egg interactions studied with the scanning electron microscope.
1973,
Pubmed
,
Echinobase
Tegner,
Scanning electron microscope studies of sea urchin fertilization. I. Eggs with vitelline layers.
1976,
Pubmed
,
Echinobase
Terho,
Method for determination of the sulfate content of glycosaminoglycans.
1971,
Pubmed
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
Vacquier,
The isolation and preliminary analysis of the hyaline layer of sea urchin eggs.
1969,
Pubmed
,
Echinobase
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Weber,
Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.
1972,
Pubmed
Wolf,
Isolation, physicochemical properties, and the macromolecular composition of the vitelline and fertilization envelopes from Xenopus laevis eggs.
1976,
Pubmed