ECB-ART-52269Dev Growth Differ 1984 Jan 01;266:571-574. doi: 10.1111/j.1440-169X.1984.00571.x.
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Intracellular Localization and Sedimentation Coefficient of DNA Ligase in Oocytes of the Starfish, Asterina pectinifera: (starfish/DNA ligase/germinal vesicle).
When immature oocytes of Asterina pectinifera were separated into karyoplasts (nuclear fragments) and cytoplasts (anuclear fragments) by cytochalasin B treatment and centrifugation in a sucrose gradient, almost all their DNA ligase activity was recovered in the karyoplasts. Thus, DNA ligase seems to be localized in the germinal vesicles or their vicinity in starfish oocytes. No ontogenic change in the activity of DNA ligase per oocyte, egg or embryo, or in its sedimentation coefficient (4.1 S) was observed during oocyte maturation, fertilization, or early development.
PubMed ID: 37280939
Article link: Dev Growth Differ