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Echinobase
ECB-ART-33109
J Cell Biol 1970 Mar 01;443:635-45. doi: 10.1083/jcb.44.3.635.
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The isolation of a major structural element of the sea urchin fertilization membrane.

Bryan J .


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Procedures for isolating the contents of the cortical granules from the ova of the sea urchin, Strongylocentrotus purpuratus, are reported. Dithiothreitol is used to remove the vitelline coat; the "demembranated" eggs are then subsequently activated with butyric acid. By means of these procedures, the hyaline protein and crystalline or paracrystalline material have been isolated from the cortical granules. The crystalline material consists of sheets of cylinders or tubules 150-200 A in diameter. This material is believed to be a major structural element of the fertilization membrane which, in the absence of the vitelline coat, does not form.

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Genes referenced: LOC100887844

References [+] :
Anderson, Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction. 1968, Pubmed, Echinobase