Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Species-specific inhibition of fertilization by a peptide derived from the sperm protein bindin.
Minor JE
,
Britten RJ
,
Davidson EH
.
???displayArticle.abstract???
The sperm protein bindin is responsible for the species-specific adhesion of the sperm to the egg. The regions of the bindin molecule responsible for forming the contact between the sperm and the egg were investigated by measuring the ability of peptides representing various regions of the bindin sequence to inhibit fertilization. Twenty-four peptides were studied: 7 based on the Strongylocentrotus purpuratus bindin sequence, 11 based on the S. franciscanus bindin sequence, and 6 control peptides. Values for the concentration of peptide required to inhibit 50% of the productive sperm contacts (IC50) were extracted from experimental measurements of the extent of fertilization in the presence of various concentrations. of these peptides. The IC50 value averaged 220 microM for the control peptides. Active peptides representing certain specific subregions of the bindin sequence displayed IC50 values < 10% of the average value for control peptides, and the IC50 for the most potent of the peptides tested was only approximately 1% of the control peptide value (IC50 = 2.2 microM). Furthermore, we found that a peptide representing a particular region of the S. franciscanus bindin sequence that differs from the S. purpuratus bindin sequence inhibits fertilization species specifically. For the reaction of S. purpuratus sperm and eggs, the IC50 of this peptide was approximately 120 microM, whereas for the reaction of S. franciscanus sperm and eggs it was only 8.6 microM. These results demonstrate that a few specific regions of the bindin molecule are involved in the sperm-egg contact and that certain of these regions mediate the species specificity of the interaction in a sequence-specific manner.
Buus,
The relation between major histocompatibility complex (MHC) restriction and the capacity of Ia to bind immunogenic peptides.
1987, Pubmed
Buus,
The relation between major histocompatibility complex (MHC) restriction and the capacity of Ia to bind immunogenic peptides.
1987,
Pubmed
Cameron,
Locale and level of bindin mRNA in maturing testis of the sea urchin, Strongylocentrotus purpuratus.
1990,
Pubmed
,
Echinobase
DeAngelis,
Role of basic amino acids in the interaction of bindin with sulfated fucans.
1988,
Pubmed
,
Echinobase
DeAngelis,
Specific recognition of sulfate esters by bindin, a sperm adhesion protein from sea urchins.
1990,
Pubmed
,
Echinobase
DeAngelis,
Zinc specifically stimulates the selective binding of a peptide analog of bindin to sulfated fucans.
1990,
Pubmed
,
Echinobase
Foltz,
Purification and characterization of an extracellular fragment of the sea urchin egg receptor for sperm.
1990,
Pubmed
,
Echinobase
Gao,
Sequence of mRNA coding for bindin, a species-specific sea urchin sperm protein required for fertilization.
1986,
Pubmed
,
Echinobase
Glabe,
Egg surface glycoprotein receptor for sea urchin sperm bindin.
1978,
Pubmed
,
Echinobase
Glabe,
A supramolecular theory for specificity in intracellular adhesion.
1979,
Pubmed
,
Echinobase
Glabe,
Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles. I. Specific association of bindin with gel-phase phospholipid vesicles.
1985,
Pubmed
,
Echinobase
Glabe,
Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles. II. Bindin induces the fusion of mixed-phase vesicles that contain phosphatidylcholine and phosphatidylserine in vitro.
1985,
Pubmed
,
Echinobase
Glabe,
The sequence of the Arbacia punctulata bindin cDNA and implications for the structural basis of species-specific sperm adhesion and fertilization.
1991,
Pubmed
,
Echinobase
Hamm,
Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit.
1988,
Pubmed
Kennedy,
Analysis of the membrane-interacting domain of the sea urchin sperm adhesive protein bindin.
1989,
Pubmed
,
Echinobase
Kinsey,
Studies on the specificity of sperm binding in echinoderm fertilization.
1980,
Pubmed
,
Echinobase
König,
Three cytoplasmic loops of rhodopsin interact with transducin.
1989,
Pubmed
Lentz,
Synthetic peptides in the study of the interaction of rabies virus and the acetylcholine receptor.
1988,
Pubmed
Matsumura,
Proteasome (multicatalytic proteinase) of sea urchin sperm and its possible participation in the acrosome reaction.
1991,
Pubmed
,
Echinobase
Minor,
Comparison of the bindin proteins of Strongylocentrotus franciscanus, S. purpuratus, and Lytechinus variegatus: sequences involved in the species specificity of fertilization.
1991,
Pubmed
,
Echinobase
Moy,
Immunoperoxidase localization of bindin during the adhesion of sperm to sea urchin eggs.
1979,
Pubmed
,
Echinobase
Nishioka,
Localization of bindin expression during sea urchin spermatogenesis.
1990,
Pubmed
,
Echinobase
Roe,
Evidence for involvement of metalloendoproteases in a step in sea urchin gamete fusion.
1988,
Pubmed
,
Echinobase
Rossignol,
Characterization of the sperm receptor on the surface of eggs of Strongylocentrotus purpuratus.
1984,
Pubmed
,
Echinobase
SeGall,
Chemical characterization of the component of the jelly coat from sea urchin eggs responsible for induction of the acrosome reaction.
1979,
Pubmed
,
Echinobase
Summers,
Species-specificity of acrosome reaction and primary gamete binding in echinoids.
1975,
Pubmed
,
Echinobase
Vacquier,
Methods for quantitating sea urchin sperm-egg binding.
1973,
Pubmed
,
Echinobase
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
Vacquier,
Handling, labeling, and fractionating sea urchin spermatozoa.
1986,
Pubmed
,
Echinobase
Vacquier,
Protease released from sea urchin eggs at fertilization alters the vitelline layer and aids in preventing polyspermy.
1973,
Pubmed
,
Echinobase
Ward,
Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer.
1985,
Pubmed
,
Echinobase
Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed
Yool,
Alteration of ionic selectivity of a K+ channel by mutation of the H5 region.
1991,
Pubmed
