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Nucleic Acids Res
1993 Jun 25;2112:2801-8. doi: 10.1093/nar/21.12.2801.
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Two distinct, sequence-specific DNA-binding proteins interact independently with the major replication pause region of sea urchin mtDNA.
Qureshi SA
,
Jacobs HT
.
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We have identified a second DNA-binding protein in sea urchin embryo mitochondria, which interacts with a binding site in the major replication pause region, at the junction of the genes for ATP synthase subunit 6 and cytochrome c oxidase subunit III (COIII). We provisionally designate this protein mtPBP2, to distinguish it from the previously characterized mitochondrial pause-region binding protein mtPBP1, whose properties and binding site are quite distinct. The high-affinity binding site for mtPBP2 lies at the 5'' end of the COIII gene, and exhibits partial dyad symmetry, although modification interference analysis indicates that recognition is complex. Binding of mtPBP2 to this site induces a bend of approximately 45 degrees in the DNA. Southwestern blots show that mtPBP1 and 2 are both single polypeptides, of apparent molecular weights 25 kD and 18 kD respectively. In vitro, mtPBP1 and mtPBP2 bind independently to their high-affinity sites, which are separated by about 50 bp.
Cantatore,
A novel gene order in the Paracentrotus lividus mitochondrial genome.
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Cantatore,
A novel gene order in the Paracentrotus lividus mitochondrial genome.
1987,
Pubmed
,
Echinobase
Cantatore,
The complete nucleotide sequence, gene organization, and genetic code of the mitochondrial genome of Paracentrotus lividus.
1989,
Pubmed
,
Echinobase
Clayton,
Replication of animal mitochondrial DNA.
1982,
Pubmed
Fisher,
Purification and characterization of human mitochondrial transcription factor 1.
1988,
Pubmed
Fisher,
A rapid, efficient method for purifying DNA-binding proteins. Denaturation-renaturation chromatography of human and yeast mitochondrial extracts.
1991,
Pubmed
Hager,
Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.
1980,
Pubmed
Harrison,
DNA recognition by proteins with the helix-turn-helix motif.
1990,
Pubmed
Jacobs,
Nucleotide sequence and gene organization of sea urchin mitochondrial DNA.
1988,
Pubmed
,
Echinobase
Jacobs,
Sea urchin egg mitochondrial DNA contains a short displacement loop (D-loop) in the replication origin region.
1989,
Pubmed
,
Echinobase
Khatri,
The replication terminator protein of E. coli is a DNA sequence-specific contra-helicase.
1989,
Pubmed
Kim,
Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector.
1989,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lane,
Use of gel retardation to analyze protein-nucleic acid interactions.
1992,
Pubmed
Maxam,
Sequencing end-labeled DNA with base-specific chemical cleavages.
1980,
Pubmed
Mayhook,
Replication origins and pause sites in sea urchin mitochondrial DNA.
1992,
Pubmed
,
Echinobase
Parisi,
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
1991,
Pubmed
Qureshi,
Characterization of a high-affinity binding site for a DNA-binding protein from sea urchin embryo mitochondria.
1993,
Pubmed
,
Echinobase
Roberti,
Identification of two homologous mitochondrial DNA sequences, which bind strongly and specifically to a mitochondrial protein of Paracentrotus lividus.
1991,
Pubmed
,
Echinobase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Shuey,
Characterization of an RNA polymerase activity from HeLa cell mitochondria, which initiates transcription at the heavy strand rRNA promoter and the light strand promoter in human mitochondrial DNA.
1985,
Pubmed
Siebenlist,
Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.
1980,
Pubmed
Truss,
Contacts between steroid hormone receptors and thymines in DNA: an interference method.
1990,
Pubmed
Vilette,
DNA transcription and repressor binding affect deletion formation in Escherichia coli plasmids.
1992,
Pubmed
Vinson,
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
1988,
Pubmed
Welter,
DNA curvature in front of the human mitochondrial L-strand replication origin with specific protein binding.
1989,
Pubmed
Wong,
In vitro replication of human mitochondrial DNA: accurate initiation at the origin of light-strand synthesis.
1985,
Pubmed