Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
EMBO J
2005 Aug 03;2415:2741-52. doi: 10.1038/sj.emboj.7600744.
Show Gene links
Show Anatomy links
Ca2+ spikes in the flagellum control chemotactic behavior of sperm.
Böhmer M
,
Van Q
,
Weyand I
,
Hagen V
,
Beyermann M
,
Matsumoto M
,
Hoshi M
,
Hildebrand E
,
Kaupp UB
.
???displayArticle.abstract???
The events that occur during chemotaxis of sperm are only partly known. As an essential step toward determining the underlying mechanism, we have recorded Ca2+ dynamics in swimming sperm of marine invertebrates. Stimulation of the sea urchin Arbacia punctulata by the chemoattractant or by intracellular cGMP evokes Ca2+ spikes in the flagellum. A Ca2+ spike elicits a turn in the trajectory followed by a period of straight swimming (''turn-and-run''). The train of Ca2+ spikes gives rise to repetitive loop-like movements. When sperm swim in a concentration gradient of the attractant, the Ca2+ spikes and the stimulus function are synchronized, suggesting that precise timing of Ca2+ spikes controls navigation. We identified the peptide asterosap as a chemotactic factor of the starfish Asterias amurensis. The Ca2+ spikes and swimming behavior of sperm from starfish and sea urchin are similar, implying that the signaling pathway of chemotaxis has been conserved for almost 500 million years.
Brokaw,
Calcium ion regulation of flagellar beat symmetry in reactivated sea urchin spermatozoa.
1974, Pubmed,
Echinobase
Brokaw,
Calcium ion regulation of flagellar beat symmetry in reactivated sea urchin spermatozoa.
1974,
Pubmed
,
Echinobase
Brokaw,
Calcium-induced asymmetrical beating of triton-demembranated sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Cook,
Activation of Ca2+ permeability by cAMP is coordinated through the pHi increase induced by speract.
1993,
Pubmed
,
Echinobase
Cook,
Sperm chemotaxis: egg peptides control cytosolic calcium to regulate flagellar responses.
1994,
Pubmed
,
Echinobase
Cook,
Selective modulation by cGMP of the K+ channel activated by speract.
1993,
Pubmed
,
Echinobase
Crenshaw,
Kinematics of helical motion of microorganisms capable of motion with four degrees of freedom.
1989,
Pubmed
Darszon,
Ion transport in sperm signaling.
2001,
Pubmed
Darszon,
Ion channels in sperm physiology.
1999,
Pubmed
Eisenbach,
Towards understanding the molecular mechanism of sperm chemotaxis.
2004,
Pubmed
Harper,
Stimulation of human spermatozoa with progesterone gradients to simulate approach to the oocyte. Induction of [Ca(2+)](i) oscillations and cyclical transitions in flagellar beating.
2004,
Pubmed
Ishikawa,
Strategies for sperm chemotaxis in the siphonophores and ascidians: a numerical simulation study.
2004,
Pubmed
Kaupp,
The signal flow and motor response controling chemotaxis of sea urchin sperm.
2003,
Pubmed
,
Echinobase
Kirkman-Brown,
How to attract a sperm.
2003,
Pubmed
Matsui,
Induction of the Acrosome Reaction in Starfish: (acrosome reaction/starfish sperm/egg jelly/Ca-channel/ionophore).
1986,
Pubmed
,
Echinobase
Matsumoto,
A sperm-activating peptide controls a cGMP-signaling pathway in starfish sperm.
2003,
Pubmed
,
Echinobase
Miller,
An N-terminal partial sequence of the 13 kDa Pycnopodia helianthoides sperm chemoattractant 'startrak' possesses sperm-attracting activity.
1996,
Pubmed
,
Echinobase
Nishigaki,
Real-time measurements of the interactions between fluorescent speract and its sperm receptor.
2000,
Pubmed
,
Echinobase
Nishigaki,
Structure and function of asterosaps, sperm-activating peptides from the jelly coat of starfish eggs.
1996,
Pubmed
,
Echinobase
Nishigaki,
A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase.
2004,
Pubmed
,
Echinobase
Schackmann,
Alteration of intracellular [Ca2+] in sea urchin sperm by the egg peptide speract. Evidence that increased intracellular Ca2+ is coupled to Na+ entry and increased intracellular pH.
1986,
Pubmed
,
Echinobase
Shiba,
Sperm-activating peptide induces asymmetric flagellar bending in sea urchin sperm.
2005,
Pubmed
,
Echinobase
Shimizu,
Identification and Characterization of Putative Receptors for Sperm-Activating Peptide I (SAP-I) in Spermatozoa of the Sea Urchin Hemicentrotus pulcherrimus1.
1994,
Pubmed
,
Echinobase
Shimomura,
Differential effects of resact analogues on sperm respiration rates and cyclic nucleotide concentrations.
1986,
Pubmed
,
Echinobase
Solzin,
Revisiting the role of H+ in chemotactic signaling of sperm.
2004,
Pubmed
,
Echinobase
Spehr,
Identification of a testicular odorant receptor mediating human sperm chemotaxis.
2003,
Pubmed
Steinier,
Smoothing and differentiation of data by simplified least square procedure.
1972,
Pubmed
Su,
A flagellar K(+)-dependent Na(+)/Ca(2+) exchanger keeps Ca(2+) low in sea urchin spermatozoa.
2002,
Pubmed
,
Echinobase
Suzuki,
Structure, function and biosynthesis of sperm-activating peptides and fucose sulfate glycoconjugate in the extracellular coat of sea urchin eggs.
1995,
Pubmed
,
Echinobase
Wada,
Phylogenetic relationships among extant classes of echinoderms, as inferred from sequences of 18S rDNA, coincide with relationships deduced from the fossil record.
1994,
Pubmed
,
Echinobase
Ward,
Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer.
1985,
Pubmed
,
Echinobase
Wood,
Speract induces calcium oscillations in the sperm tail.
2003,
Pubmed
,
Echinobase
Yoshida,
Store-operated calcium channel regulates the chemotactic behavior of ascidian sperm.
2003,
Pubmed
