Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Biol Cell
2000 Mar 01;113:897-914. doi: 10.1091/mbc.11.3.897.
Show Gene links
Show Anatomy links
Dynamics of the endoplasmic reticulum and golgi apparatus during early sea urchin development.
Terasaki M
.
???displayArticle.abstract???
The endoplasmic reticulum (ER) and Golgi were labeled by green fluorescent protein chimeras and observed by time-lapse confocal microscopy during the rapid cell cycles of sea urchin embryos. The ER undergoes a cyclical microtubule-dependent accumulation at the mitotic poles and by photobleaching experiments remains continuous through the cell cycle. Finger-like indentations of the nuclear envelope near the mitotic poles appear 2-3 min before the permeability barrier of the nuclear envelope begins to change. This permeability change in turn is approximately 30 s before nuclear envelope breakdown. During interphase, there are many scattered, disconnected Golgi stacks throughout the cytoplasm, which appear as 1- to 2-microm fluorescent spots. The number of Golgi spots begins to decline soon after nuclear envelope breakdown, reaches a minimum soon after cytokinesis, and then rapidly increases. At higher magnification, smaller spots are seen, along with increased fluorescence in the ER. Quantitative measurements, along with nocodazole and photobleaching experiments, are consistent with a redistribution of some of the Golgi to the ER during mitosis. The scattered Golgi coalesce into a single large aggregate during the interphase after the ninth embryonic cleavage; this is likely to be preparatory for secretion of the hatching enzyme during the following cleavage cycle.
Acharya,
Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis.
1998, Pubmed
Acharya,
Signaling via mitogen-activated protein kinase kinase (MEK1) is required for Golgi fragmentation during mitosis.
1998,
Pubmed
Aridor,
Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport.
1995,
Pubmed
Bacallao,
The subcellular organization of Madin-Darby canine kidney cells during the formation of a polarized epithelium.
1989,
Pubmed
Berlin,
Surface functions during Mitosis I: phagocytosis, pinocytosis and mobility of surface-bound Con A.
1978,
Pubmed
Burke,
A monoclonal antibody against a 135-K Golgi membrane protein.
1982,
Pubmed
Burkhardt,
Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution.
1997,
Pubmed
Cole,
Diffusional mobility of Golgi proteins in membranes of living cells.
1996,
Pubmed
Cole,
Golgi dispersal during microtubule disruption: regeneration of Golgi stacks at peripheral endoplasmic reticulum exit sites.
1996,
Pubmed
Cole,
Retrograde transport of Golgi-localized proteins to the ER.
1998,
Pubmed
Collins,
Epithelial differentiation in the mouse preimplantation embryo: making adhesive cell contacts for the first time.
1995,
Pubmed
Dailey,
Structure and organization of membrane organelles along distal microtubule segments in growth cones.
1991,
Pubmed
Dan,
CELL CYCLE STUDY UP TO THE TIME OF HATCHING IN THE EMBRYOS OF THE SEA URCHIN, HEMICENTROTUS PULCHERRIMUS.
1980,
Pubmed
,
Echinobase
Ellenberg,
Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis.
1997,
Pubmed
Farmaki,
Forward and retrograde trafficking in mitotic animal cells. ER-Golgi transport arrest restricts protein export from the ER into COPII-coated structures.
1999,
Pubmed
Featherstone,
Newly synthesized G protein of vesicular stomatitis virus is not transported to the Golgi complex in mitotic cells.
1985,
Pubmed
Fuseler,
Repetitive procurement of mature gametes from individual sea stars and sea urchins.
1973,
Pubmed
,
Echinobase
Georgatos,
Nuclear envelope breakdown in mammalian cells involves stepwise lamina disassembly and microtubule-drive deformation of the nuclear membrane.
1997,
Pubmed
Gibbins,
Microtubules in the formation and development of the primary mesenchyme in Arbacia punctulata. I. The distribution of microtubules.
1969,
Pubmed
,
Echinobase
Groigno,
An anaphase calcium signal controls chromosome disjunction in early sea urchin embryos.
1998,
Pubmed
,
Echinobase
HIRAMOTO,
Microinjection of the live spermatozoa into sea urchin eggs.
1962,
Pubmed
,
Echinobase
Harris,
The role of membranes in the ogranization of the mitotic apparatus.
1975,
Pubmed
,
Echinobase
Heim,
Improved green fluorescence.
1995,
Pubmed
Henson,
A calsequestrin-like protein in the endoplasmic reticulum of the sea urchin: localization and dynamics in the egg and first cell cycle embryo.
1989,
Pubmed
,
Echinobase
Hepler,
Membranes in the mitotic apparatus: their structure and function.
1984,
Pubmed
Hiller,
Golgi detection in mitotic and interphase cells by antibodies to secreted galactosyltransferase.
1982,
Pubmed
Hirschberg,
Kinetic analysis of secretory protein traffic and characterization of golgi to plasma membrane transport intermediates in living cells.
1998,
Pubmed
Hsu,
A brefeldin A-like phenotype is induced by the overexpression of a human ERD-2-like protein, ELP-1.
1992,
Pubmed
Ito,
Ultrastructure and 3H-thymidine incorporation by chromosome vesicles in sea urchin embryos.
1981,
Pubmed
,
Echinobase
Jesch,
The Golgi and endoplasmic reticulum remain independent during mitosis in HeLa cells.
1998,
Pubmed
Kiehart,
Microinjection of echinoderm eggs: apparatus and procedures.
1982,
Pubmed
,
Echinobase
Lepage,
Early expression of a collagenase-like hatching enzyme gene in the sea urchin embryo.
1990,
Pubmed
,
Echinobase
Lepage,
Spatial expression of the hatching enzyme gene in the sea urchin embryo.
1992,
Pubmed
,
Echinobase
Lepage,
Purification and characterization of the sea urchin embryo hatching enzyme.
1989,
Pubmed
,
Echinobase
Lippincott-Schwartz,
Building a secretory apparatus: role of ARF1/COPI in Golgi biogenesis and maintenance.
1998,
Pubmed
Lippincott-Schwartz,
Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER.
1989,
Pubmed
Lowe,
Golgi division and membrane traffic.
1998,
Pubmed
Lucero,
ERcalcistorin/protein disulfide isomerase (PDI). Sequence determination and expression of a cDNA clone encoding a calcium storage protein with PDI activity from endoplasmic reticulum of the sea urchin egg.
1994,
Pubmed
,
Echinobase
Masuda,
SPECIES SPECIFIC PATTERN OF CILIOGENESIS IN DEVELOPING SEA URCHIN EMBRYOS.
1979,
Pubmed
,
Echinobase
Misteli,
Mitotic disassembly of the Golgi apparatus in vivo.
1995,
Pubmed
Munro,
A C-terminal signal prevents secretion of luminal ER proteins.
1987,
Pubmed
Presley,
Golgi membrane dynamics.
1998,
Pubmed
Presley,
ER-to-Golgi transport visualized in living cells.
1997,
Pubmed
Preston,
Regulation of Ca2+ influx during mitosis: Ca2+ influx and depletion of intracellular Ca2+ stores are coupled in interphase but not mitosis.
1991,
Pubmed
Regier,
Absolute rates of protein synthesis in sea urchins with specific activity measurements of radioactive leucine and leucyl-tRNA.
1977,
Pubmed
,
Echinobase
Roe,
Biosynthesis and secretion of the hatching enzyme during sea urchin embryogenesis.
1990,
Pubmed
,
Echinobase
Semenza,
ERD2, a yeast gene required for the receptor-mediated retrieval of luminal ER proteins from the secretory pathway.
1990,
Pubmed
Shima,
An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle.
1998,
Pubmed
Shima,
Partitioning of the Golgi apparatus during mitosis in living HeLa cells.
1997,
Pubmed
Sluder,
The role of spindle microtubules in the timing of the cell cycle in echinoderm eggs.
1986,
Pubmed
,
Echinobase
Stanley,
The mechanism of Golgi segregation during mitosis is cell type-specific.
1997,
Pubmed
Storrie,
Recycling of golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering.
1998,
Pubmed
Summers,
A Stereometric Analysis of Karyokinesis, Cytokinesis and Cell Arrangements during and following Fourth Cleavage Period in the Sea Urchin, Lytechinus variegatus: (sea urchin embryo/cell division patterns/stereo imaging/3-D reconstruction).
1993,
Pubmed
,
Echinobase
Swanson,
Nuclear reassembly excludes large macromolecules.
1987,
Pubmed
Terasaki,
Structural change of the endoplasmic reticulum during fertilization: evidence for loss of membrane continuity using the green fluorescent protein.
1996,
Pubmed
,
Echinobase
Terasaki,
Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.
1991,
Pubmed
,
Echinobase
Terasaki,
Redistribution of cytoplasmic components during germinal vesicle breakdown in starfish oocytes.
1994,
Pubmed
,
Echinobase
Terasaki,
Microtubules and the endoplasmic reticulum are highly interdependent structures.
1986,
Pubmed
Thyberg,
Reorganization of the Golgi complex in association with mitosis: redistribution of mannosidase II to the endoplasmic reticulum and effects of brefeldin A.
1992,
Pubmed
Townsley,
Mutational analysis of the human KDEL receptor: distinct structural requirements for Golgi retention, ligand binding and retrograde transport.
1993,
Pubmed
Warren,
Mitotic disassembly of the mammalian Golgi apparatus.
1995,
Pubmed
Warren,
Membrane partitioning during cell division.
1993,
Pubmed
Warren,
Organelle inheritance.
1996,
Pubmed
Wooding,
The dynamics of golgi protein traffic visualized in living yeast cells.
1998,
Pubmed
Yang,
Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis.
1997,
Pubmed
Zaal,
Golgi membranes are absorbed into and reemerge from the ER during mitosis.
1999,
Pubmed
Zeligs,
Mitosis in rat thyroid epithelial cells in vivo. I. Ultrastructural changes in cytoplasmic organelles during the mitotic cycle.
1979,
Pubmed