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Malays Orthop J
2013 Mar 01;71:41-8. doi: 10.5704/MOJ.1303.015.
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The In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line.
Shahrulazua A
,
Samsudin A
,
Iskandar M
,
Amran A
.
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UNLABELLED: Despite its claimed therapeutic effects, the action of sea cucumber (known as gamat in the Malay language) on human osteoblast cells is still unknown. We performed in vitro studies utilising extract of Stichopus sp1 (gamat) to elucidate its effects on cell viability and functional activity. We found an inverse relationship between gamat concentration and its effect on osteoblast cell viability (p<0.001). Only gamat concentration at 1mg/ml significantly promoted cell viability at day 3 of incubation. There was a trend towards increased osteoblast cell function in the presence of gamat at 5mg/ml and 10mg/ml but this observation was not consistent at different incubation periods.
KEY WORDS: Alkaline phosphatase, Stichopus, Sea Cucumbers, Osteoblasts, Tetrazolium Salts.
Table I. : Mean chemical analysis values for gamat extract (dissolved in distilled water)
Table II. : Mean osmolarity and pH values for various solutions used in the study
Table III. : Multiple comparisons of the effect of various gamat concentrations on osteoblast cell viability after several
incubation periods (post hoc Bonferroni test)
Table IV. : Multiple comparisons of the effect of various gamat concentrations on osteoblast functional activity after several
incubation periods (post hoc Bonferroni test)
Fig. 3. : Microscopic appearance of osteoblast cells after three days of incubation period, viewed under x20 magnification, in their
corresponding growth media (A, negative control;B, gamat at 1.6mg/ml; C,gamat at 25mg/ml; D, gamat at 50mg/ml; E, gamat
at 100mg/ml; F=positive control).
Fig. 4. : Effect on osteoblast cells viability by various gamat
concentrations after different incubation periods.
Fig. 5. : Effect on osteoblast functional activity by various gamat
concentrations after different incubation periods.
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