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J Cell Biol
1982 Mar 01;923:733-41. doi: 10.1083/jcb.92.3.733.
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Mechanochemical coupling in the relaxation of rigor-wave sea urchin sperm flagella.
Penningroth SM
,
Cheung A
,
Olehnik K
,
Koslosky R
.
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The relaxation (straightening) of flagellar rigor waves, which is known to be induced by micromolar ATP concentrations was investigated with respect to its dependence on the binding and hydrolysis of ATP. Flagellar rigor waves were formed by the dilution of demembranated, reactivated sea urchin (Lytechinus pictus) spermatozoa into ATP-free buffer. Relaxation in response to nucleotide was quantitated by measuring theta, the mean flagellar bend angle per sperm; this novel assay permitted determination of the rate of relaxation. It was found that (a) the rate of flagellar relaxation induced by 4 X 10(-6) M ATP was inhibited 80% by vanadate concentrations of 3 X 10(-6) M and above; (b) of 16 hydrolyzable and nonhydrolyzable nucleotide di-, tri-, and tetraphosphates tested, only three, each of which was hydrolyzed by the flagellar axonemal ATPase activity (ATP, dATP, and epsilon-ATP) were also capable of effecting relaxation; (c) several hundred ATP molecules were estimated to be hydrolyzed by each dynein of ATP hydrolysis, which defines the efficiency of ATP utilization, increased 30-fold as the ATP relaxation depends on ATP hydrolysis; (b) because it depends on ATP hydrolysis, flagellar relaxation is an inappropriate model system for investigating the role of ATP binding in the mechanochemical cycle of dynein; and (c) the efficiency of mechanochemical coupling in flagellar motility is an ATP-dependent phenomenon. A general model of relaxation is proposed based on active microtubule sliding.
Bouchard,
erythro-9-[3-(2-Hydroxynonyl)]adenine is an inhibitor of sperm motility that blocks dynein ATPase and protein carboxylmethylase activities.
1981, Pubmed,
Echinobase
Bouchard,
erythro-9-[3-(2-Hydroxynonyl)]adenine is an inhibitor of sperm motility that blocks dynein ATPase and protein carboxylmethylase activities.
1981,
Pubmed
,
Echinobase
Brokaw,
Adenosine triphosphate usage by flagella.
1967,
Pubmed
,
Echinobase
Brokaw,
Localized activation of bending in proximal, medial and distal regions of sea-urchin sperm flagella.
1973,
Pubmed
,
Echinobase
Brokaw,
Mechanochemical coupling in flagella. V. Effects of viscosity on movement and ATP-dephosphorylation of Triton-demembranated sea-urchin spermatozoa.
1977,
Pubmed
,
Echinobase
Brokaw,
Mechanochemical coupling in flagella. I. Movement-dependent dephosphorylation of ATP by glycerinated spermatozoa.
1968,
Pubmed
Eisenberg,
A cross-bridge model of muscle contraction.
1978,
Pubmed
Gibbons,
Flagellar movement and adenosine triphosphatase activity in sea urchin sperm extracted with triton X-100.
1972,
Pubmed
,
Echinobase
Gibbons,
Properties of flagellar "rigor waves" formed by abrupt removal of adenosine triphosphate from actively swimming sea urchin sperm.
1974,
Pubmed
,
Echinobase
Gibbons,
The molecular basis of flagellar motility in sea urchin spermatozoa.
1975,
Pubmed
,
Echinobase
Gibbons,
Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.
1978,
Pubmed
,
Echinobase
Gibbons,
A latent adenosine triphosphatase form of dynein 1 from sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Huxley,
Muscular contraction.
1974,
Pubmed
Kobayashi,
Inhibition of dynein ATPase by vanadate, and its possible use as a probe for the role of dynein in cytoplasmic motility.
1978,
Pubmed
,
Echinobase
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Okuno,
Effects of AMPPNP and vanadate on the mechanochemical crossbridge cycle in flagella.
1981,
Pubmed
,
Echinobase
Okuno,
Inhibition and relaxation of sea urchin sperm flagella by vanadate.
1980,
Pubmed
,
Echinobase
Penningroth,
Effects of adenylyl imidodiphosphate, a nonhydrolyzable adenosine triphosphate analog, on reactivated and rigor wave sea urchin sperm.
1978,
Pubmed
,
Echinobase
Penningroth,
ATP formation from adenyl-5'-yl imidodiphosphate, a nonhydrolyzable ATP analog.
1980,
Pubmed
Penningroth,
Nucleotide binding and phosphorylation in microtubule assembly in vitro.
1977,
Pubmed
Sale,
Study of the mechanism of vanadate inhibition of the dynein cross-bridge cycle in sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Takahashi,
Kinetic properties of dynein ATPase from Tetrahymena pyriformis. The initial phosphate burst of dynein ATPase and its interaction with ATP analogs.
1979,
Pubmed
Watanabe,
Nucleotide-metabolizing enzymes in Chlamydomonas flagella.
1976,
Pubmed
