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J Cell Biol
1981 Mar 01;883:604-17. doi: 10.1083/jcb.88.3.604.
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Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system.
Kiehart DP
.
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I microinjected calcium ions into echinoderm eggs during mitosis to determine the calcium sensitivity of microtubules (Mts) in vivo. Spindle birefringence (BR), a measure of the number of aligned Mts in the spindle, is locally, rapidly, and reversibly abolished by small volumes of microinjected CaCl2 (1 mM). Rapid return of BR is followed by anaphase, and subsequent divisions are normal. Similar doses of MgCl2, BaCl2, KCl, NaCl, pH buffers, distilled water, or vegetable oil have no effect on spindle BR, whereas large doses of such agents sometimes cause slow, uniform loss in BR over the course of a minute or more. Of the ions tested, only Sr++ causes effects comparable to Ca++. Ca-EGTA buffers, containing greater than micromolar free Ca++, abolishes BR in a manner similar to millimolar concentrations of injected CaCl2. Caffeine, a potent uncoupler of the Ca++-pump/ATPase of sarcoplasmic reticulum, causes a local, transient depression in spindle BR in the injected region. Finally, injection of potassium oxalate results in the formation of small, highly BR crystals, presumably CA-oxalate, in Triton-sensitive compartments in the cytoplasm. Taken together, these findings demonstrate that spindle Mts are sensitive to levels of free Ca++ in the physiological range, provide evidence for the existence of a strong cytoplasmic Ca++-sequestering system, and support the notion that Mt assembly and disassembly in local regions of the spindle may be orchestrated by local changes in the cytoplasmic free Ca++ concentration during mitosis. An appendix offers the design of a new chamber for immobilizing echinoderm eggs for injection, a new method for determining the volume of the injected solution, and a description of the microinjection technique, which was designed, but never fully described, by Hiramoto (Y. Hiramoto, Exp. Cell. Res., 1962, 27:416-426.).
Allen,
Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component.
1977, Pubmed
Allen,
Aequorin luminescence: relation of light emission to calcium concentration--a calcium-independent component.
1977,
Pubmed
Begg,
Micromanipulation studies of chromosome movement. I. Chromosome-spindle attachment and the mechanical properties of chromosomal spindle fibers.
1979,
Pubmed
Bergen,
Head-to-tail polymerization of microtubules in vitro. Electron microscope analysis of seeded assembly.
1980,
Pubmed
Bergen,
Polarity of microtubules nucleated by centrosomes and chromosomes of Chinese hamster ovary cells in vitro.
1980,
Pubmed
Blaustein,
Calcium buffering in presynaptic nerve terminals. I. Evidence for involvement of a nonmitochondrial ATP-dependent sequestration mechanism.
1978,
Pubmed
Brinley,
Mitochondria and other calcium buffers of squid axon studied in situ.
1978,
Pubmed
COSTANTIN,
LOCALIZATION OF CALCIUM-ACCUMULATING STRUCTURES IN STRIATED MUSCLE FIBERS.
1965,
Pubmed
Ettienne,
Subcellular localization of calcium repositories in plasmodia of the acellular slime mold Physarum polycephalum.
1972,
Pubmed
Fuge,
The arrangement of microtubules and the attachment of chromosomes to the spindle during anaphase in tipulid spermatocytes.
1974,
Pubmed
Fuge,
Ultrastructure of the mitotic spindle.
1977,
Pubmed
Fuller,
Structure and control of assembly of cytoplasmic microtubules in normal and transformed cells.
1976,
Pubmed
Fuseler,
Repetitive procurement of mature gametes from individual sea stars and sea urchins.
1973,
Pubmed
,
Echinobase
HIRAMOTO,
Microinjection of the live spermatozoa into sea urchin eggs.
1962,
Pubmed
,
Echinobase
Haga,
Polymerization and depolymerization of microtubules in vitro as studied by flow birefringence.
1974,
Pubmed
Harris,
The role of membranes in the ogranization of the mitotic apparatus.
1975,
Pubmed
,
Echinobase
Head,
Calcium-binding modulator protein from the unfertilized egg of the sea urchin Arbacia punctulata.
1979,
Pubmed
,
Echinobase
Heidemann,
Aster formation in eggs of Xenopus laevis. Induction by isolated basal bodies.
1975,
Pubmed
Henkart,
Endoplasmic reticulum sequesters calcium in the squid giant axon.
1978,
Pubmed
Hepler,
Membranes in the mitotic apparatus of barley cells.
1980,
Pubmed
Inoué,
Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement.
1967,
Pubmed
Inoué,
Growth and lability of Chaetopterus oocyte mitotic spindles isolated in the presence of porcine brain tubulin.
1974,
Pubmed
Inoué,
Functional organization of mitotic microtubules. Physical chemistry of the in vivo equilibrium system.
1975,
Pubmed
,
Echinobase
Inoué,
Mitosis in Barbulanympha. II. Dynamics of a two-stage anaphase, nuclear morphogenesis, and cytokinesis.
1978,
Pubmed
Johnson,
The equilibrium assembly of microtubules in vitro.
1975,
Pubmed
Kinoshita,
The behaviour and localization of intracellular relaxing system during cleavage in the sea urchin egg.
1967,
Pubmed
,
Echinobase
Marcum,
Control of microtubule assembly-disassembly by calcium-dependent regulator protein.
1978,
Pubmed
Margolis,
Opposite end assembly and disassembly of microtubules at steady state in vitro.
1978,
Pubmed
McGill,
Human chromosomes and centrioles as nucleating sites for the in vitro assembly of microtubules from bovine brain tubulin.
1975,
Pubmed
Means,
Calmodulin--an intracellular calcium receptor.
1980,
Pubmed
Nath,
Studies on cyclic AMP levels and phosphodiesterase activity in developing sea urchin eggs. Effects of puromycin, 6-dimethylamino purine and aminophylline.
1973,
Pubmed
,
Echinobase
Nath,
Studies on the uptake and metabolism of adenosine 3':5'-cyclic monophosphate and N6,O2-dibutyryl 3':5'-cyclic adenosine monophosphate in sea urchin eggs.
1973,
Pubmed
,
Echinobase
Olmsted,
Ionic and nucleotide requirements for microtubule polymerization in vitro.
1975,
Pubmed
PODOLSKY,
REGULATION BY CALCIUM OF THE CONTRACTION AND RELAXATION OF MUSCLE FIBERS.
1964,
Pubmed
PORTZEHL,
THE DEPENDENCE OF CONTRACTION AND RELAXATION OF MUSCLE FIBRES FROM THE CRAB MAIA SQUINADO ON THE INTERNAL CONCENTRATION OF FREE CALCIUM IONS.
1964,
Pubmed
Petzelt,
Biochemistry of the mitotic spindle.
1979,
Pubmed
Rebhun,
Reversible restoration of the birefringence of cold-treated, isolated mitotic apparatus of surf clam eggs with chick brain tubulin.
1974,
Pubmed
Rebhun,
Regulation of size and birefringence of the in vivo mitotic apparatus.
1974,
Pubmed
,
Echinobase
Rebhun,
Regulation of the in vivo mitotic apparatus by glycols and metabolic inhibitors.
1975,
Pubmed
,
Echinobase
Rose,
Calcium ion distribution in cytoplasm visualised by aequorin: diffusion in cytosol restricted by energized sequestering.
1975,
Pubmed
Rosenfeld,
Magnesium stimulation of calcium binding to tubulin and calcium induced depolymerization of microtubules.
1976,
Pubmed
SUTHERLAND,
Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles.
1958,
Pubmed
Salmon,
Pressure-induced depolymerization of spindle microtubules. I. Changes in birefringence and spindle length.
1975,
Pubmed
Salmon,
Spindle microtubules: thermodynamics of in vivo assembly and role in chromosome movement.
1975,
Pubmed
,
Echinobase
Salmon,
Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).
1980,
Pubmed
,
Echinobase
Sato,
Microtubular origin of mitotic spindle form birefringence. Demonstration of the applicability of Wiener's equation.
1975,
Pubmed
,
Echinobase
Schliwa,
The role of divalent cations in the regulation of microtubule assembly. In vivo studies on microtubules of the heliozoan axopodium using the ionophore A23187.
1976,
Pubmed
Silver,
Isolation of mitotic apparatus containing vesicles with calcium sequestration activity.
1980,
Pubmed
,
Echinobase
Snyder,
Initiation and growth of microtubules from mitotic centers in lysed mammalian cells.
1975,
Pubmed
Tilney,
How microtubule patterns are generated. The relative importance of nucleation and bridging of microtubules in the formation of the axoneme of Raphidiophrys.
1971,
Pubmed
Timasheff,
In vitro assembly of cytoplasmic microtubules.
1980,
Pubmed
Weber,
The relationship between caffeine contracture of intact muscle and the effect of caffeine on reticulum.
1968,
Pubmed
Weber,
The mechanism of the action of caffeine on sarcoplasmic reticulum.
1968,
Pubmed
Wegner,
Head to tail polymerization of actin.
1976,
Pubmed
Weisenberg,
Microtubule formation in vitro in solutions containing low calcium concentrations.
1972,
Pubmed
Weisenberg,
In vitro polymerization of microtubules into asters and spindles in homogenates of surf clam eggs.
1975,
Pubmed
Weisenberg,
Tubulin-nucleotide interactions during the polymerization and depolymerization of microtubules.
1976,
Pubmed
Weisenberg,
Role of nucleotide hydrolysis in microtubule assembly.
1976,
Pubmed
Welsh,
Tubulin and calmodulin. Effects of microtubule and microfilament inhibitors on localization in the mitotic apparatus.
1979,
Pubmed
Wolniak,
Detection of the membrane-calcium distribution during mitosis in Haemanthus endosperm with chlorotetracycline.
1980,
Pubmed
