Biochem Biophys Res Commun 2001 Jun 08;2842:531-5. doi: 10.1006/bbrc.2001.5000.

Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry.

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Speract, a decapeptide from sea urchin egg jelly, induces various sperm responses. Stopped-flow fluorometry was used to examine the binding of labeled speract and the intracellular changes in pH (pH(i)) and Ca2+ ([Ca2+]i) it induces in sperm. We observed significant time delays for the increase in pH(i) and [Ca2+]i induced by 200 nM speract (69 and 190 ms, respectively). Also, we found that the receptor undergoes a pH(i)-dependent affinity change at around 129 ms. These time delays probably reflect biochemical processes underlying each sperm response to speract that circumscribe the time sequence of the signaling events.

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Genes referenced: LOC100887844 LOC576642