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Dev Growth Differ
1986 Feb 01;281:85-94. doi: 10.1111/j.1440-169X.1986.00085.x.
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Purification of Acetylcholinesterase from Sea Urchin (Hemicentrotus pulcherrimus) Embryos by Affinity Chromatography: (acethylcholinesterase/purification/Sea Urchin, Hemicentrotus pulcherrimus).
Akasaka K
,
Sasaki H
,
Shimada H
,
Shiroya T
.
Abstract
Only one form of acetylcholinesterase (AchE) was detected in Hemicentrotus pulcherrimus embryos. In H. pulcherrimus embryos as well as in the other sea urchin embryos, AchE activity begins to increase rapidly after gastrula stage. Purification of AchE from plutei has been carried out by the procedure including affinity chromatography. Purified AchE had the activity 14,600 times higher than that of homogenate, and the final yield of AchE was 8%. The enzyme seems to be electrophoretically homogeneous, and has a molecular weight of 3 × 105 as determined by Sepharose CL-6B column chromatography.
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